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Antimicrobial Agents and Chemotherapy, August 2007, p. 2997-3000, Vol. 51, No. 8
0066-4804/07/$08.00+0     doi:10.1128/AAC.00111-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Increased Expression of ampC in Pseudomonas aeruginosa Mutants Selected with Ciprofloxacin{triangledown}

Daniel J. Wolter, Amber J. Schmidtke, Nancy D. Hanson, and Philip D. Lister*

Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, Nebraska 68178

Received 15 January 2007/ Returned for modification 12 March 2007/ Accepted 14 May 2007

Two Pseudomonas aeruginosa mutants exhibiting increased expression of ampC were selected during exposure to ciprofloxacin. These mutants also exhibited significant increases in mexCD-oprJ expression, but further studies failed to show a link between the increased expression of mexCD-oprJ and ampC. Increased ampC expression was not related to mutations within ampR, the ampC-ampR intergenic region, ampD, ampDh2, or ampDh3 or to changes in the levels of expression of these amidase genes. However, ampD complementation restored wild-type levels of ampC expression and ceftazidime susceptibility, suggesting alternative mechanisms of ampC regulation.

* Corresponding author. Mailing address: Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178. Phone: (402) 280-1224. Fax: (402) 280-1875. E-mail: pdlister{at}creighton.edu

{triangledown} Published ahead of print on 21 May 2007.

Antimicrobial Agents and Chemotherapy, August 2007, p. 2997-3000, Vol. 51, No. 8
0066-4804/07/$08.00+0     doi:10.1128/AAC.00111-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lister, P. D., Wolter, D. J., Hanson, N. D. (2009). Antibacterial-Resistant Pseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms. Clin. Microbiol. Rev. 22: 582-610 [Abstract] [Full Text]  
  • Schmidtke, A. J., Hanson, N. D. (2008). Role of ampD Homologs in Overproduction of AmpC in Clinical Isolates of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 52: 3922-3927 [Abstract] [Full Text]  


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