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Antimicrobial Agents and Chemotherapy, September 2007, p. 3374-3377, Vol. 51, No. 9
0066-4804/07/$08.00+0     doi:10.1128/AAC.00275-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Update to the Multiplex PCR Strategy for Assignment of mec Element Types in Staphylococcus aureus{triangledown}

Catarina Milheiriço,1 Duarte C. Oliveira,1* and Hermínia de Lencastre1,2

Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal,1 Laboratory of Microbiology, The Rockefeller University, New York, New York 100212

Received 23 February 2007/ Returned for modification 7 April 2007/ Accepted 10 June 2007

Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strategy for SCCmec typing that we described in 2002 so that SCCmec types IV and V may be properly identified.

* Corresponding author. Mailing address: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal. Phone: (351) 21 446 9862. Fax: (351) 21 442 8766. E-mail: dco{at}itqb.unl.pt

{triangledown} Published ahead of print on 18 June 2007.

Antimicrobial Agents and Chemotherapy, September 2007, p. 3374-3377, Vol. 51, No. 9
0066-4804/07/$08.00+0     doi:10.1128/AAC.00275-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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