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Update to the Multiplex PCR Strategy for Assignment of mec Element Types in Staphylococcus aureus

Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal,¹ Laboratory of Microbiology, The Rockefeller University, New York, New York 10021²

Received 23 February 2007/ Returned for modification 7 April 2007/ Accepted 10 June 2007

Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strategy for SCCmec typing that we described in 2002 so that SCCmec types IV and V may be properly identified.

Published ahead of print on 18 June 2007.

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