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Antimicrobial Agents and Chemotherapy, September 2007, p. 3410-3412, Vol. 51, No. 9
0066-4804/07/$08.00+0     doi:10.1128/AAC.00362-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Determination of the Primary Target of a Quinolone Drug and the Effect of Quinolone Resistance-Conferring Mutations by Measuring Quinolone Sensitivity Based on Its Mode of Action{triangledown}

Emily S. Pfeiffer{dagger} and Hiroshi Hiasa*

Department of Pharmacology, University of Minnesota Medical School—Twin Cities, Minneapolis, Minnesota 55455

Received 18 March 2007/ Returned for modification 10 June 2007/ Accepted 20 June 2007

We used an assay to measure quinolone sensitivity as a shift in the position of the cleavage-religation equilibrium. This assay was found to be useful in identifying the primary target of a quinolone drug and assessing the effect of quinolone resistance-conferring mutations.

* Corresponding author. Mailing address: 6-120 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455. Phone: (612) 626-3101. Fax: (612) 625-8408. E-mail: hiasa001{at}umn.edu

{triangledown} Published ahead of print on 2 July 2007.

{dagger} Present address: MGI Pharma, Bloomington, Minnesota.

Antimicrobial Agents and Chemotherapy, September 2007, p. 3410-3412, Vol. 51, No. 9
0066-4804/07/$08.00+0     doi:10.1128/AAC.00362-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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