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Antimicrobial Agents and Chemotherapy, September 2007, p. 3425-3430, Vol. 51, No. 9
0066-4804/07/$08.00+0     doi:10.1128/AAC.00340-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Detection of Azithromycin Resistance in Treponema pallidum by Real-Time PCR{triangledown}

Mark W. Pandori,1* Charmie Gordones,2 Lina Castro,1 Joseph Engelman,1 Mark Siedner,3 Sheila Lukehart,2 and Jeffrey Klausner1

San Francisco Department of Public Health, San Francisco, California,1 Department of Medicine, University of Washington, Seattle, Washington,2 Johns Hopkins School of Public Health, Baltimore, Maryland3

Received 13 March 2007/ Returned for modification 30 April 2007/ Accepted 2 July 2007

We describe a real-time PCR assay for the discrimination of azithromycin-resistant and -susceptible strains of Treponema pallidum. This assay is rapid and allows for as many as 30 clinical specimens to be analyzed simultaneously without the need for DNA sequencing.

* Corresponding author. Mailing address: Public Health Laboratory, San Francisco Department of Public Health, 101 Grove St., Room 419, San Francisco, CA 94102-4592. Phone: (415) 554-2800. Fax: (415) 431-0651. E-mail: Mark_Pandori{at}sfdph.org

{triangledown} Published ahead of print on 9 July 2007.

Antimicrobial Agents and Chemotherapy, September 2007, p. 3425-3430, Vol. 51, No. 9
0066-4804/07/$08.00+0     doi:10.1128/AAC.00340-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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