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Antimicrobial Agents and Chemotherapy, November 2008, p. 4145-4148, Vol. 52, No. 11
0066-4804/08/$08.00+0     doi:10.1128/AAC.00959-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Pyrosequencing To Detect Mutations in FKS1 That Confer Reduced Echinocandin Susceptibility in Candida albicans{triangledown}

Nathan P. Wiederhold,1,2* Jodi L. Grabinski,1,2 Guillermo Garcia-Effron,3 David S. Perlin,3 and Samuel A. Lee4

University of Texas at Austin College of Pharmacy, Austin, Texas,1 University of Texas Health Science Center at San Antonio, Pharmacotherapy Education and Research Center, San Antonio, Texas,2 Public Health Research Institute, New Jersey Medical School—UMDNJ, Newark, New Jersey,3 University of New Mexico Health Science Center, Albuquerque, New Mexico4

Received 7 July 2008/ Returned for modification 4 August 2008/ Accepted 4 September 2008

Pyrosequencing was compared to Sanger dideoxy sequencing to detect mutations in FKS1 responsible for reduced echinocandin susceptibility in Candida albicans. These methods were in complete agreement for 10 of 12 clinical isolates with elevated echinocandin MICs, supporting the potential feasibility of pyrosequencing to detect mutations within diploid fungi.

* Corresponding author. Mailing address: UTHSCSA, PERC MSC 6220, 7703 Floyd Curl Drive, San Antonio, TX 78229. Phone: (210) 567-8340. Fax: (210) 567-8328. E-mail: wiederholdn{at}uthscsa.edu

{triangledown} Published ahead of print on 15 September 2008.

Antimicrobial Agents and Chemotherapy, November 2008, p. 4145-4148, Vol. 52, No. 11
0066-4804/08/$08.00+0     doi:10.1128/AAC.00959-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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