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Antimicrobial Agents and Chemotherapy, November 2008, p. 4155-4158, Vol. 52, No. 11
0066-4804/08/$08.00+0     doi:10.1128/AAC.00082-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Real-Time PCR Detection of gyrA and parC Mutations in Streptococcus pneumoniae{triangledown}

S. Page,1,2 F. Vernel-Pauillac,2 O. O'Connor,1 S. Bremont,3 F. Charavay,1 P. Courvalin,3 C. Goarant,2 and S. Le Hello1*

Institut Pasteur de Nouvelle-Calédonie, Laboratoire d'Epidémiologie Moléculaire,1 Laboratoire de Recherche en Bactériologie, Nouméa, New Caledonia,2 Institut Pasteur, Unité des Agents Antibactériens, Paris, France3

Received 20 January 2008/ Returned for modification 30 March 2008/ Accepted 12 August 2008

Fluoroquinolone resistance in Streptococcus pneumoniae mainly involves stepwise mutations predominantly in the parC and gyrA genes. We have developed a single-run real-time PCR assay for detection of the four most common mutations in the quinolone resistance-determining regions of these genes. This assay provides a useful tool for both clinical and epidemiological use.

* Corresponding author. Mailing address: Institut Pasteur de Nouvelle-Calédonie, 9-11 avenue Paul Doumer, BP 61, 98845 Nouméa Cedex, New Caledonia. Phone: 687.27.75.32. Fax: 687.27.33.90. E-mail: slehello{at}pasteur.nc

{triangledown} Published ahead of print on 25 August 2008.

Antimicrobial Agents and Chemotherapy, November 2008, p. 4155-4158, Vol. 52, No. 11
0066-4804/08/$08.00+0     doi:10.1128/AAC.00082-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lysnyansky, I., Mikula, I., Gerchman, I., Levisohn, S. (2009). Rapid Detection of a Point Mutation in the parC Gene Associated with Decreased Susceptibility to Fluoroquinolones in Mycoplasma bovis. Antimicrob. Agents Chemother. 53: 4911-4914 [Abstract] [Full Text]  


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