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Antimicrobial Agents and Chemotherapy, November 2008, p. 4194-4197, Vol. 52, No. 11
0066-4804/08/$08.00+0     doi:10.1128/AAC.01337-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

KHM-1, a Novel Plasmid-Mediated Metallo-β-Lactamase from a Citrobacter freundii Clinical Isolate{triangledown}

Jun-ichiro Sekiguchi,1 Koji Morita,2* Tomoe Kitao,1 Noboru Watanabe,2 Mitsuhiro Okazaki,3 Tohru Miyoshi-Akiyama,1 Masato Kanamori,2 and Teruo Kirikae1

Department of Infectious Diseases, Research Institute, International Medical Center of Japan, Shinjuku, Tokyo 162-8655, Japan,1 Department of Microbiology, Kyorin University School of Health Sciences, Hachioji, Tokyo 192-8508, Japan,2 Department of Clinical Laboratory, Kyorin University Hospital, Mitaka, Tokyo 181-8611, Japan3

Received 17 October 2007/ Returned for modification 2 December 2007/ Accepted 1 August 2008

A novel gene, blaKHM-1, encoding a metallo-β-lactamase, KHM-1, was cloned from a clinical isolate of Citrobacter freundii resistant to most β-lactam antibiotics. Escherichia coli expressing blaKHM-1 was resistant to all broad-spectrum β-lactams except for monobactams and showed reduced susceptibility to carbapenems. Recombinant KHM-1 exhibited EDTA-inhibitable hydrolytic activity against most β-lactams, with an overall preference for cephalosporins.

* Corresponding author. Mailing address: Department of Microbiology, Kyorin University School of Health Sciences, 476 Miyashita-cho, Hachioji, Tokyo 192-8508, Japan. Phone: (81) 42 691 0011, ext. 4125. Fax: (81) 42 691 1094. E-mail: moritako{at}kyorin-u.ac.jp

{triangledown} Published ahead of print on 2 September 2008.

Antimicrobial Agents and Chemotherapy, November 2008, p. 4194-4197, Vol. 52, No. 11
0066-4804/08/$08.00+0     doi:10.1128/AAC.01337-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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