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Antimicrobial Agents and Chemotherapy, December 2008, p. 4486-4491, Vol. 52, No. 12
0066-4804/08/$08.00+0     doi:10.1128/AAC.00222-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Complex Ciprofloxacin Resistome Revealed by Screening a Pseudomonas aeruginosa Mutant Library for Altered Susceptibility{triangledown}

Elena B. M. Breidenstein, Bhavjinder K. Khaira, Irith Wiegand, Joerg Overhage, and Robert E. W. Hancock*

Centre for Microbial Diseases and Immunity Research, University of British Columbia, 2259 Lower Mall, Vancouver, BC, Canada

Received 16 February 2008/ Returned for modification 27 April 2008/ Accepted 16 September 2008

Pseudomonas aeruginosa offers substantial therapeutic challenges due to its high intrinsic resistance to many antibiotics and its propensity to develop mutational and/or adaptive resistance. The PA14 comprehensive mutant library was screened for mutants exhibiting either two- to eightfold increased susceptibilities (revealing genes involved in intrinsic resistance) or decreased susceptibilities (mutational resistance) to the fluoroquinolone ciprofloxacin. Thirty-five and 79 mutants with increased and decreased susceptibilities, respectively, were identified, as confirmed by broth dilution.

* Corresponding author. Mailing address: Centre for Microbial Diseases and Immunity Research, University of British Columbia, Room 232, 2259 Lower Mall, Lower Mall Research Station, Vancouver, British Columbia V6T 1Z4, Canada. Phone: (604) 822-2682. Fax: (604) 827-5566. E-mail: bob{at}cmdr.ubc.ca

{triangledown} Published ahead of print on 29 September 2008.

Antimicrobial Agents and Chemotherapy, December 2008, p. 4486-4491, Vol. 52, No. 12
0066-4804/08/$08.00+0     doi:10.1128/AAC.00222-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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