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Antimicrobial Agents and Chemotherapy, December 2008, p. 4510-4513, Vol. 52, No. 12
0066-4804/08/$08.00+0 doi:10.1128/AAC.01073-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.
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Department of Pathology, Hershey Medical Center, P.O. Box 850, Hershey, Pennsylvania 17033
Received 8 August 2008/ Returned for modification 7 September 2008/ Accepted 29 September 2008
All 982 methicillin-resistant Staphylococcus aureus strains collected from August 2006 to December 2007 were tested for vancomycin susceptibility by using 3-µg/ml vancomycin brain heart infusion screening plates, a vancomycin Etest, and a vancomycin/teicoplanin macro Etest. Three vancomycin-intermediate Staphylococcus aureus (VISA) (0.3%) and two heterogeneous VISA (0.2%) isolates were identified. The screening method yielded 895 cases of
1 colony and 87 positive results (with growth of >1 colony after 48 h); further Etests showed 82/87 isolates with growth on screening plates to be false positive. Repeat testing showed a false-positivity rate of only 15 of the original 87 isolates by plate screening.
Published ahead of print on 6 October 2008.
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