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Antimicrobial Agents and Chemotherapy, December 2008, p. 4510-4513, Vol. 52, No. 12
0066-4804/08/$08.00+0     doi:10.1128/AAC.01073-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Incidence and Characteristics of Vancomycin Nonsusceptible Strains of Methicillin-Resistant Staphylococcus aureus at Hershey Medical Center{triangledown} ,{dagger}

Klaudia Kosowska-Shick, Lois M. Ednie, Pamela McGhee, Kathy Smith, Cynthia D. Todd, Amanda Wehler, and Peter C. Appelbaum*

Department of Pathology, Hershey Medical Center, P.O. Box 850, Hershey, Pennsylvania 17033

Received 8 August 2008/ Returned for modification 7 September 2008/ Accepted 29 September 2008

All 982 methicillin-resistant Staphylococcus aureus strains collected from August 2006 to December 2007 were tested for vancomycin susceptibility by using 3-µg/ml vancomycin brain heart infusion screening plates, a vancomycin Etest, and a vancomycin/teicoplanin macro Etest. Three vancomycin-intermediate Staphylococcus aureus (VISA) (0.3%) and two heterogeneous VISA (0.2%) isolates were identified. The screening method yielded 895 cases of ≤1 colony and 87 positive results (with growth of >1 colony after 48 h); further Etests showed 82/87 isolates with growth on screening plates to be false positive. Repeat testing showed a false-positivity rate of only 15 of the original 87 isolates by plate screening.


* Corresponding author. Mailing address: Department of Pathology H160, Hershey Medical Center, P.O. Box 850, Hershey, PA 17033. Phone: (717) 531-5113. Fax: (717) 531-7953. E-mail: pappelbaum{at}psu.edu

{triangledown} Published ahead of print on 6 October 2008.

{dagger} Supplemental material for this article may be found at http://aac.asm.org/.


Antimicrobial Agents and Chemotherapy, December 2008, p. 4510-4513, Vol. 52, No. 12
0066-4804/08/$08.00+0     doi:10.1128/AAC.01073-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.