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Antimicrobial Agents and Chemotherapy, February 2008, p. 753-756, Vol. 52, No. 2
0066-4804/08/$08.00+0     doi:10.1128/AAC.00914-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Combined Real-Time PCR and Pyrosequencing Strategy for Objective, Sensitive, Specific, and High-Throughput Identification of Reduced Susceptibility to Penicillins in Neisseria meningitidis{triangledown}

Sara Thulin,* Per Olcén, Hans Fredlund, and Magnus Unemo

National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Örebro University Hospital, Örebro, Sweden

Received 13 July 2007/ Returned for modification 11 October 2007/ Accepted 24 November 2007

A segment of penA in Neisseria meningitidis strains (n = 127), including two nucleotide sites closely associated to reduced susceptibility to penicillins, was amplified and pyrosequenced. All results were in concordance with Sanger sequencing, and a high correlation between alterations in the two Peni-specific sites and reduced susceptibility to penicillins was identified.

* Corresponding author. Mailing address: Department of Clinical Microbiology, Örebro University Hospital, SE-701 85 Örebro, Sweden. Phone: 46 19 602 15 20. Fax: 46 19 12 74 16. E-mail: sara.thulin{at}orebroll.se

{triangledown} Published ahead of print on 10 December 2007.

Antimicrobial Agents and Chemotherapy, February 2008, p. 753-756, Vol. 52, No. 2
0066-4804/08/$08.00+0     doi:10.1128/AAC.00914-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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