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Antimicrobial Agents and Chemotherapy, February 2008, p. 782-785, Vol. 52, No. 2
0066-4804/08/$08.00+0     doi:10.1128/AAC.01613-06
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Intracellular Efavirenz Levels in Peripheral Blood Mononuclear Cells from Human Immunodeficiency Virus-Infected Individuals{triangledown}

Rie Tanaka,1 Hideji Hanabusa,2 Ei Kinai,2 Naoki Hasegawa,1 Masayoshi Negishi,1 and Shingo Kato1*

Keio University School of Medicine,1 Ogikubo Hospital, Tokyo, Japan2

Received 27 December 2006/ Returned for modification 15 March 2007/ Accepted 28 November 2007

We describe a novel method for isolating plasma-free peripheral blood mononuclear cells retaining intracellular efavirenz. Quantification of efavirenz in 13 human immunodeficiency virus-infected patients by liquid chromatography-tandem mass spectrometry showed a higher correlation of intracellular levels with unbound plasma levels (accumulation ratio, 1,190) than with total plasma levels.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. Phone: 81 3 5363 3769. Fax: 81 3 5360 1508. E-mail: skato{at}sc.itc.keio.ac.jp

{triangledown} Published ahead of print on 10 December 2007.

Antimicrobial Agents and Chemotherapy, February 2008, p. 782-785, Vol. 52, No. 2
0066-4804/08/$08.00+0     doi:10.1128/AAC.01613-06
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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