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Antimicrobial Agents and Chemotherapy, March 2008, p. 1198-1200, Vol. 52, No. 3
0066-4804/08/$08.00+0     doi:10.1128/AAC.00682-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Induction of L1 and L2 β-Lactamases of Stenotrophomonas maltophilia{triangledown}

Rouh-Mei Hu,1 Kuang-Jay Huang,1 Lii-Tzu Wu,2 Ying-Ju Hsiao,3 and Tsuey-Ching Yang3*

Department of Biotechnology and Bioinformatics, Asia University, Taichung 413, Taiwan,1 Department of Microbiology, China Medical University, Taichung 404, Taiwan,2 Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan3

Received 24 May 2007/ Returned for modification 4 August 2007/ Accepted 1 December 2007

Isogenic L1 and L2 gene knockout mutants of Stenotrophomonas maltophilia KJ (KJ{Delta}L1 and KJ{Delta}L2, respectively) were constructed by xylE gene replacement. Induction kinetics of the L1 and L2 genes were evaluated by testing catechol 2,3-dioxygenase activity in the mutants. The results suggested that the induction of the L1 and L2 genes was differentially regulated.

* Corresponding author. Mailing address: China Medical University, 91 Hsueh-Shih Rd., Taichung, Taiwan 40402, ROC. Phone: 886-4-2205-3366, ext. 7203. Fax: 886-4-2205-7414. E-mail: tcyang{at}mail.cmu.edu.tw

{triangledown} Published ahead of print on 17 December 2007.

Antimicrobial Agents and Chemotherapy, March 2008, p. 1198-1200, Vol. 52, No. 3
0066-4804/08/$08.00+0     doi:10.1128/AAC.00682-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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