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Pyrazinamide Resistance and pncA Gene Mutations in Mycobacterium tuberculosis

Pontus Juréen,^1,2,* Jim Werngren,^1, Juan-Carlos Toro,¹ and Sven Hoffner¹

Department of Bacteriology, Swedish Institute for Infectious Disease Control, Solna,¹ Department of Microbiology, Tumour and Cell Biology Center, Karolinska Institute, Stockholm, Sweden²

Received 25 January 2008/ Returned for modification 20 February 2008/ Accepted 26 February 2008

Thirty-four pyrazinamide-resistant and 37 pyrazinamide-susceptible Mycobacterium tuberculosis complex strains were analyzed for pncA gene mutations. None of the sensitive strains had any mutations, apart from silent mutations, whereas all but one resistant strain showed pncA mutations. By using sequencing as a means of early resistance detection, the inconsistency of phenotypic pyrazinamide assays can be circumvented.

Published ahead of print on 3 March 2008.

P.J. and J.W. contributed equally to this study.