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Antimicrobial Agents and Chemotherapy, June 2008, p. 1952-1956, Vol. 52, No. 6
0066-4804/08/$08.00+0     doi:10.1128/AAC.01463-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Characterization of a Naturally Occurring Class D β-Lactamase from Achromobacter xylosoxidans{triangledown}

Yohei Doi,1,2 Laurent Poirel,1 David L. Paterson,2,3 and Patrice Nordmann1*

Service de Bactériologie-Virologie, INSERM U914, Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, 94275 K.-Bicêtre, France,1 Division of Infectious Diseases, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania,2 University of Queensland, Royal Brisbane and Women's Hospital, Brisbane, Australia3

Received 12 November 2007/ Returned for modification 2 February 2008/ Accepted 16 March 2008

A chromosomally encoded class D β-lactamase, OXA-114, was characterized from Achromobacter xylosoxidans strain CIP69598. β-Lactamase OXA-114 shared 56% amino acid identity with the naturally occurring class D β-lactamase of Burkholderia cenocepacia and 42% identity with the acquired oxacillinases OXA-9 and OXA-18. OXA-114 has a narrow-spectrum hydrolysis profile, although it includes imipenem, at a very low level. PCR and sequencing revealed that blaOXA-114-like genes were identified in all A. xylosoxidans strains tested (n = 5), indicating that this β-lactamase is naturally occurring in that species. Induction experiments with imipenem and cefoxitin did not show inducibility of blaOXA-114 expression.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.aphp.fr

{triangledown} Published ahead of print on 24 March 2008.

Antimicrobial Agents and Chemotherapy, June 2008, p. 1952-1956, Vol. 52, No. 6
0066-4804/08/$08.00+0     doi:10.1128/AAC.01463-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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