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Antimicrobial Agents and Chemotherapy, June 2008, p. 2236-2238, Vol. 52, No. 6
0066-4804/08/$08.00+0     doi:10.1128/AAC.01680-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Apoptosis Induced by Aminoglycosides in LLC-PK1 Cells: Comparative Study of Neomycin, Gentamicin, Amikacin, and Isepamicin Using Electroporation{triangledown} ,{dagger}

Sophie Denamur, Françoise Van Bambeke, Marie-Paule Mingeot-Leclercq, and Paul M. Tulkens*

Unité de Pharmacologie Cellulaire et Moléculaire, Université Catholique de Louvain, B-1200 Brussels, Belgium

Received 30 December 2007/ Returned for modification 22 March 2008/ Accepted 26 March 2008

Levels of apoptosis induction (4',6'-diamidino-2-phenylindole staining, activation of caspase 3) for aminoglycosides were compared by using renal LLC-PK1 cells. Amikacin caused less apoptosis than gentamicin in incubated cells. In electroporated cells, neomycin B and gentamicin caused apoptosis in the 0.03 to 0.1 mM range, isepamicin required larger concentrations (0.2 mM), and amikacin was without effect.

* Corresponding author. Mailing address: UCL 7370, Ave. E. Mounier 73, B-1200 Brussels, Belgium. Phone: 32-2-762-2136. Fax: 32-2-764-7373. E-mail: tulkens{at}facm.ucl.ac.be

{triangledown} Published ahead of print on 7 April 2008.

{dagger} Supplemental material for this article may be found at http://aac.asm.org/.

Antimicrobial Agents and Chemotherapy, June 2008, p. 2236-2238, Vol. 52, No. 6
0066-4804/08/$08.00+0     doi:10.1128/AAC.01680-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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