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Antimicrobial Agents and Chemotherapy, July 2008, p. 2573-2580, Vol. 52, No. 7
0066-4804/08/$08.00+0     doi:10.1128/AAC.00393-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Acquisition of a Plasmid-Borne blaOXA-58 Gene with an Upstream IS1008 Insertion Conferring a High Level of Carbapenem Resistance to Acinetobacter baumannii{triangledown}

Te-Li Chen,1,2 Roy Chen-Chih Wu,2 Men-Fang Shaio,3 Chang-Phone Fung,1,2 and Wen-Long Cho2*

Division of Infectious Diseases,1 Department of Internal Medicine, Taipei Veterans General Hospital, Institute of Tropical Medicine, School of Medicine, National Yang-Ming University, Taipei,2 Division of Clinical Research, Department of Medical Research, Kuang Tien General Hospital, Institute of Clinical Nutrition, Hung Kuang University, Taichung, Taiwan3

Received 23 March 2008/ Returned for modification 7 April 2008/ Accepted 16 April 2008

The oxacillinase gene was reported to confer limited resistance to carbapenem in Acinetobacter baumannii. In this study, we have demonstrated that an A. baumannii clinical isolate harboring a plasmid, pTVICU53, has 11,037 bp encoding 13 open reading frames. A blaOXA-58 gene with an upstream insertion of truncated ISAba3 ({Delta}ISAba3) and IS1008 was found in this plasmid. {Delta}ISAba3and IS1008 provided two independent promoters for the transcription control of the blaOXA-58 gene. The transformation of pTVICU53 or a shuttle vector bearing IS1008-{Delta}ISAba3-blaOXA-58 to different A. baumannii recipients can increase their MICs of carbapenem 64- to 256-fold. The deletion of promoters provided by IS1008 resulted in dramatic decreases in blaOXA-58 transcription and a 32- to 64-fold reduction in the carbapenem MIC. These findings highlight that A. baumannii might develop carbapenem resistance with a single transformation step, taking up a plasmid containing a genetic construct with a potentially high level of transcription of the blaOXA-58 gene.

* Corresponding author. Mailing address: Institute of Tropical Medicine, Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan. Phone: 886-2-28267173. Fax: 886-2-28214670. E-mail: wenlong{at}ym.edu.tw

{triangledown} Published ahead of print on 28 April 2008.

Antimicrobial Agents and Chemotherapy, July 2008, p. 2573-2580, Vol. 52, No. 7
0066-4804/08/$08.00+0     doi:10.1128/AAC.00393-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lu, P.-L., Doumith, M., Livermore, D. M., Chen, T.-P., Woodford, N. (2009). Diversity of carbapenem resistance mechanisms in Acinetobacter baumannii from a Taiwan hospital: spread of plasmid-borne OXA-72 carbapenemase. J Antimicrob Chemother 63: 641-647 [Abstract] [Full Text]  
  • Zarrilli, R., Vitale, D., Di Popolo, A., Bagattini, M., Daoud, Z., Khan, A. U., Afif, C., Triassi, M. (2008). A Plasmid-Borne blaOXA-58 Gene Confers Imipenem Resistance to Acinetobacter baumannii Isolates from a Lebanese Hospital. Antimicrob. Agents Chemother. 52: 4115-4120 [Abstract] [Full Text]  


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