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Antimicrobial Agents and Chemotherapy, August 2008, p. 2750-2754, Vol. 52, No. 8
0066-4804/08/$08.00+0     doi:10.1128/AAC.00044-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Antimicrobial Susceptibilities of Geographically Diverse Clinical Human Isolates of Leptospira{triangledown}

Roseanne A. Ressner,1 Matthew E. Griffith,1 Miriam L. Beckius,1 Guillermo Pimentel,2 R. Scott Miller,3 Katrin Mende,4 Susan L. Fraser,5 Renee L. Galloway,6 Duane R. Hospenthal,1 and Clinton K. Murray1*

Brooke Army Medical Center, Fort Sam Houston, Texas,1 U.S. Naval Medical Research Unit 3, Cairo, Egypt,2 Walter Reed Army Institute of Research, Washington, DC,3 Infectious Disease Clinical Research Program, Fort Sam Houston, Texas,4 Walter Reed Army Medical Center, Washington, DC,5 Centers for Disease Control and Prevention, Atlanta, Georgia6

Received 12 January 2008/ Returned for modification 12 March 2008/ Accepted 7 April 2008

Although antimicrobial therapy of leptospirosis has been studied in a few randomized controlled clinical studies, those studies were limited to specific regions of the world and few have characterized infecting strains. A broth microdilution technique for the assessment of antibiotic susceptibility has been developed at Brooke Army Medical Center. In the present study, we assessed the susceptibilities of 13 Leptospira isolates (including recent clinical isolates) from Egypt, Thailand, Nicaragua, and Hawaii to 13 antimicrobial agents. Ampicillin, cefepime, azithromycin, and clarithromycin were found to have MICs below the lower limit of detection (0.016 µg/ml). Cefotaxime, ceftriaxone, imipenem-cilastatin, penicillin G, moxifloxacin, ciprofloxacin, and levofloxacin had MIC90s between 0.030 and 0.125 µg/ml. Doxycycline and tetracycline had the highest MIC90s: 2 and 4 µg/ml, respectively. Doxycycline and tetracycline were noted to have slightly higher MICs against isolates from Egypt than against strains from Thailand or Hawaii; otherwise, the susceptibility patterns were similar. There appears to be possible variability in susceptibility to some antimicrobial agents among strains, suggesting that more extensive testing to look for geographic variability should be pursued.

* Corresponding author. Mailing address: Infectious Disease Service (MCHE-MDI), Brooke Army Medical Center, 3851 Roger Brooke Drive, Fort Sam Houston, TX 78234. Phone: (210) 916-4355. Fax: (210) 916-0388. E-mail: Clinton.Murray{at}amedd.army.mil

{triangledown} Published ahead of print on 14 April 2008.

Antimicrobial Agents and Chemotherapy, August 2008, p. 2750-2754, Vol. 52, No. 8
0066-4804/08/$08.00+0     doi:10.1128/AAC.00044-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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