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Antimicrobial Agents and Chemotherapy, August 2008, p. 2923-2925, Vol. 52, No. 8
0066-4804/08/$08.00+0     doi:10.1128/AAC.00273-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Staphylococcus aureus Cell Wall Stress Stimulon Gene-lacZ Fusion Strains: Potential for Use in Screening for Cell Wall-Active Antimicrobials{triangledown}

Rebecca Steidl, Stacy Pearson, Robert E. Stephenson, Nagender Ledala, Sutthirat Sitthisak, Brian J. Wilkinson, and Radheshyam K. Jayaswal*

Department of Biological Sciences, Illinois State University, Normal, Illinois 61790-4120

Received 27 February 2008/ Returned for modification 9 April 2008/ Accepted 30 May 2008

lacZ fusion strains were constructed using the promoters of five cell wall stress stimulon genes: pbp2, tcaA, vraSR, sgtB, and lytR. All fusion strains were induced only in the presence of cell wall-active antibiotics, suggesting the potential of these strains for use in high-throughput screening for new cell wall-active agents.

* Corresponding author. Mailing address: Department of Biological Sciences, Illinois State University, Normal, IL 61790-4120. Phone: (309) 438-5128. Fax: (309) 438-3722. E-mail: drjay{at}ilstu.edu

{triangledown} Published ahead of print on 9 June 2008.

Antimicrobial Agents and Chemotherapy, August 2008, p. 2923-2925, Vol. 52, No. 8
0066-4804/08/$08.00+0     doi:10.1128/AAC.00273-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Higgins, P. G., Rosato, A. E., Seifert, H., Archer, G. L., Wisplinghoff, H. (2009). Differential Expression of ccrA in Methicillin-Resistant Staphylococcus aureus Strains Carrying Staphylococcal Cassette Chromosome mec Type II and IVa Elements. Antimicrob. Agents Chemother. 53: 4556-4558 [Abstract] [Full Text]  


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