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Antimicrobial Agents and Chemotherapy, September 2008, p. 3424-3426, Vol. 52, No. 9
0066-4804/08/$08.00+0     doi:10.1128/AAC.00462-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Antifungal Resistance of Candida glabrata Vaginal Isolates and Development of a Quantitative Reverse Transcription-PCR-Based Azole Susceptibility Assay {triangledown}

Scott E. Gygax,1,{dagger}* John-Paul Vermitsky,1,{dagger} Sean G. Chadwick,1 Matthew J. Self,1 Jessica A. Zimmerman,3 Eli Mordechai,3 Martin E. Adelson,3 and Jason P. Trama2

Antimicrobial Resistance Division,1 Molecular and Cellular Biology Division,2 Medical Diagnostic Laboratories, L.L.C., 2439 Kuser Road, Hamilton, New Jersey 086903

Received 7 April 2008/ Returned for modification 7 June 2008/ Accepted 24 June 2008

A multiplex quantitative reverse transcription-PCR assay was developed to detect azole resistance in Candida glabrata, an important opportunistic pathogen that develops resistance rapidly. Resistance was defined as a ≥3-fold increase in CDR1 expression by this assay, which proved to be 100% sensitive and 95% specific in comparison to the gold standard broth microdilution assay.

* Corresponding author. Mailing address: Antimicrobial Resistance Division, Department of Research and Development, Medical Diagnostic Laboratories, L.L.C., 2439 Kuser Road, Hamilton, NJ 08690. Phone: (609) 570-1015. Fax: (609) 570-1030. E-mail: sgygax{at}mdlab.com

{triangledown} Published ahead of print on 30 June 2008.

{dagger} These authors have contributed equally to this work.

Antimicrobial Agents and Chemotherapy, September 2008, p. 3424-3426, Vol. 52, No. 9
0066-4804/08/$08.00+0     doi:10.1128/AAC.00462-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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