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Antimicrobial Agents and Chemotherapy, October 2009, p. 4064-4068, Vol. 53, No. 10
0066-4804/09/$08.00+0     doi:10.1128/AAC.00432-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

The Electricidal Effect Is Active in an Experimental Model of Staphylococcus epidermidis Chronic Foreign Body Osteomyelitis {triangledown}

Jose L. Del Pozo,1,2 Mark S. Rouse,1,2 Gorane Euba,1,2 Cheol-In Kang,1,2 Jayawant N. Mandrekar,3 James M. Steckelberg,1,2 and Robin Patel1,2,4*

Division of Infectious Diseases,1 Infectious Diseases Research Laboratory,2 Division of Biomedical Statistics and Informatics,3 Division of Clinical Microbiology, College of Medicine, Mayo Clinic, Rochester, Minnesota4

Received 30 March 2009/ Returned for modification 8 May 2009/ Accepted 27 July 2009

Treatment with low-amperage (200 µA) electrical current was compared to intravenous doxycycline treatment or no treatment in a rabbit model of Staphylococcus epidermidis chronic foreign body osteomyelitis to determine if the electricidal effect is active in vivo. A stainless steel implant and 104 CFU of planktonic S. epidermidis were placed into the medullary cavity of the tibia. Four weeks later, rabbits were assigned to one of three groups with treatment administered for 21 days. The groups included those receiving no treatment (n = 10), intravenous doxycycline (n = 14; 8 mg/kg of body weight three times per day), and electrical current (n = 15; 200 µA continuous delivery). Following treatment, rabbits were sacrificed and the tibias quantitatively cultured. Bacterial load was significantly reduced in the doxycycline (median, 2.55 [range, 0.50 to 6.13] log10 CFU/g of bone) and electrical-current (median, 1.09 [range, 0.50 to 2.99] log10 CFU/g of bone) groups, compared to the level for the control group (median, 4.16 [range, 3.70 to 5.66] log10 CFU/g of bone) (P < 0.0001). Moreover, treatment with electrical current was statistically significantly more efficacious (P = 0.035) than doxycycline treatment. The electricidal effect (the bactericidal activity of low-amperage electrical current against bacterial biofilms) is active in vivo in the treatment of experimental S. epidermidis chronic foreign body osteomyelitis.

* Corresponding author. Mailing address: Divisions of Clinical Microbiology and Infectious Diseases, Mayo Clinic, 200 First St. SW, Rochester, MN 55905. Phone: (507) 538-0579. Fax: (507) 284-4272. E-mail: patel.robin{at}mayo.edu

{triangledown} Published ahead of print on 3 August 2009.

Antimicrobial Agents and Chemotherapy, October 2009, p. 4064-4068, Vol. 53, No. 10
0066-4804/09/$08.00+0     doi:10.1128/AAC.00432-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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