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Antimicrobial Agents and Chemotherapy, October 2009, p. 4127-4132, Vol. 53, No. 10
0066-4804/09/$08.00+0 doi:10.1128/AAC.00616-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Evaluation of Vancomycin and Daptomycin Potency Trends (MIC Creep) against Methicillin-Resistant Staphylococcus aureus Isolates Collected in Nine U.S. Medical Centers from 2002 to 2006
Helio S. Sader,1*
Paul D. Fey,2
Douglas N. Fish,3
Ajit P. Limaye,4
George Pankey,5
James Rahal,6
Michael J. Rybak,7
David R. Snydman,8
Lisa L. Steed,9
Ken Waites,10 and
Ronald N. Jones1
JMI Laboratories, North Liberty, Iowa,1 University of Nebraska Medical Center, Omaha, Nebraska,2 University of Colorado Denver, Aurora, Colorado,3 University of Washington, Seattle, Washington,4 Ochsner Clinic Foundation, New Orleans, Louisiana,5 New York Hospital Queens, New York, New York,6 Wayne State University, Detroit, Michigan,7 Tufts Medical Center, Boston, Massachusetts,8 Medical University of South Carolina, Charleston, South Carolina,9 University of Alabama at Birmingham, Birmingham, Alabama,10
Received 6 May 2009/ Returned for modification 8 July 2009/ Accepted 16 July 2009
Vancomycin MIC creep has been reported by some institutions but not confirmed in large surveillance studies. We evaluated the possible occurrence of MIC creep when testing vancomycin and daptomycin against methicillin (oxacillin)-resistant Staphylococcus aureus (MRSA) by using precise incremental reference MIC methods. Nine hospitals (one in each U.S. census region) randomly selected bloodstream MRSA strains (target, 40/year) from 2002 to 2006. MICs were determined by the reference broth microdilution method using incremental dilutions (eight for each log2 dilution step). Isolates for which vancomycin MICs were >1 µg/ml were typed by pulsed-field gel electrophoresis (PFGE). The vancomycin MIC mode was either 0.625 µg/ml (for eight hospitals) or 0.813 µg/ml (for one hospital), and vancomycin MIC results for 72.9% of strains were between 0.563 and 0.688 µg/ml. No yearly variation in the central tendency of vancomycin MICs for the wild-type population in any medical center was observed; however, when data were analyzed by the geometric mean statistic, vancomycin MIC increases (at three sites) and declines (at three sites) were observed. The daptomycin MIC mode varied from 0.156 µg/ml (2003 to 2005) to 0.219 µg/ml (2002 and 2006), and MIC results for 83.5% (80.3 to 89.2% in each of the centers) of isolates fell between these values. Among PFGE-typed strains, 43 of 55 (78%; from seven hospitals) showed a pattern consistent with that of the USA100 clone, which was represented by all strains from two hospitals and 64 to 88% of strains from five other medical centers; only one strain (2%) was USA300. In conclusion, the perception of MIC creep may vary according to the methods used to analyze the data. Geometric mean MIC data revealed a possible, very-low-level MIC creep at three of nine sites over the 5-year period, which was not evident using modal MICs or the data from all nine hospitals (+0.02 µg/ml). The occurrence of isolates for which the vancomycin MIC was >1 µg/ml was very unusual, with no increased trend, but these organisms were usually clonal (USA100).
* Corresponding author. Mailing address: JMI Laboratories, 345 Beaver Kreek Centre, Suite A, North Liberty, IA 52317. Phone: (319) 665-3370. Fax: (319) 665-3371. E-mail: helio-sader{at}jmilabs.com
Published ahead of print on 27 July 2009.
Antimicrobial Agents and Chemotherapy, October 2009, p. 4127-4132, Vol. 53, No. 10
0066-4804/09/$08.00+0 doi:10.1128/AAC.00616-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.
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