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Antimicrobial Agents and Chemotherapy, October 2009, p. 4333-4338, Vol. 53, No. 10
0066-4804/09/$08.00+0     doi:10.1128/AAC.00260-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Novel Genetic Environment of the Carbapenem-Hydrolyzing β-Lactamase KPC-2 among Enterobacteriaceae in China{triangledown}

Ping Shen,1 Zeqing Wei,1 Yan Jiang,1,2 Xiaoxing Du,1 Shujuan Ji,1 Yunsong Yu,1* and Lanjuan Li1

State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China,1 James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou, Zhejiang 310008, China2

Received 24 February 2009/ Returned for modification 13 May 2009/ Accepted 10 July 2009

Thirty-nine blaKPC-producing isolates of the family Enterobacteriaceae with carbapenem resistance or reduced carbapenem susceptibility were obtained from inpatients from eight hospitals in six cities of three provinces in eastern China. The pulsed-field gel electrophoresis analysis of all 36 Klebsiella pneumoniae isolates revealed six major patterns. The resistant plasmids of most isolates were successfully transferred by conjugation and evaluated experimentally to be 40 to 180 kb in size. A 20.2-kb blaKPC-surrounding nucleotide sequence from plasmid pKP048 has been obtained and contains an integration structure of a Tn3-based transposon and partial Tn4401 segment, with the gene order Tn3-transposase, Tn3-resolvase, ISKpn8, the blaKPC-2 gene, and the ISKpn6-like element. The chimera of several transposon-associated elements indicated a novel genetic environment of the K. pneumoniae carbapenemase β-lactamase gene in isolates from China.

* Corresponding author. Mailing address: State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China. Phone: 86 571 8723 6421. Fax: 86 571 8723 6423. E-mail: yvys119{at}163.com

{triangledown} Published ahead of print on 20 July 2009.

Antimicrobial Agents and Chemotherapy, October 2009, p. 4333-4338, Vol. 53, No. 10
0066-4804/09/$08.00+0     doi:10.1128/AAC.00260-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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