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Antimicrobial Agents and Chemotherapy, October 2009, p. 4556-4558, Vol. 53, No. 10
0066-4804/09/$08.00+0     doi:10.1128/AAC.00395-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Differential Expression of ccrA in Methicillin-Resistant Staphylococcus aureus Strains Carrying Staphylococcal Cassette Chromosome mec Type II and IVa Elements{triangledown}

Paul G. Higgins,1 Adriana E. Rosato,2 Harald Seifert,1 Gordon L. Archer,2 and Hilmar Wisplinghoff1,2*

Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany,1 Division of Infectious Diseases, Virginia Commonwealth University, Richmond, Virginia2

Received 25 March 2009/ Returned for modification 14 May 2009/ Accepted 2 July 2009

Excision of staphylococcal cassette chromosome mec (SCCmec) is mediated through the ccrA- and -B-encoded recombinases. We investigated the effects of different antimicrobial agents on ccrA expression by using a ccrA::lacZ fusion and reverse transcription-PCR with methicillin (meticillin)-resistant Staphylococcus aureus strains MW2 (SCCmec IVa) and N315 (SCCmec II). Upregulation of ccrA was observed upon exposure to β-lactam antibiotics. Vancomycin increased ccrA expression in MW2 but had no effect on N315. Vancomycin may contribute to the transfer of SCCmec IVa but have no effect in SCCmec II.

* Corresponding author. Mailing address: Institute of Medical Microbiology, Immunology and Hygiene, University of Cologne, Goldenfelsstr. 19-21, 50935 Cologne, Germany. Phone: 49 221 478 32100. Fax: 49 221 478 32134. E-mail: h.wisplinghoff{at}uni-koeln.de

{triangledown} Published ahead of print on 13 July 2009.

Antimicrobial Agents and Chemotherapy, October 2009, p. 4556-4558, Vol. 53, No. 10
0066-4804/09/$08.00+0     doi:10.1128/AAC.00395-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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