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Antimicrobial Agents and Chemotherapy, November 2009, p. 4921-4923, Vol. 53, No. 11
0066-4804/09/$08.00+0     doi:10.1128/AAC.00862-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Comparison of Assessment of Oxygen Consumption, Etest, and CLSI M38-A2 Broth Microdilution Methods for Evaluation of the Susceptibility of Aspergillus fumigatus to Posaconazole{triangledown}

Ricardo Araujo1,2* and Ana Espinel-Ingroff3

IPATIMUP, Institute of Pathology and Molecular Immunology, University of Porto, Porto, Portugal,1 Department of Microbiology, Faculty of Medicine, University of Porto, Porto, Portugal,2 Virginia Commonwealth University Medical Center, Richmond, Virginia3

Received 25 June 2009/ Returned for modification 6 July 2009/ Accepted 17 August 2009

Posaconazole MICs for 50 Aspergillus fumigatus isolates with distinct genotypes were determined by three methods. MICs were ≥0.5 µg/ml for 5, 11, and 15 strains by the CLSI reference M38-A2, Etest (48-h), and oxygen consumption methods, respectively. The levels of categorical agreement between the results obtained by the CLSI method and those obtained by the oxygen consumption and Etest methods were 80 and 84%, respectively.

* Corresponding author. Mailing address: IPATIMUP, Institute of Pathology and Molecular Immunology, University of Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal. Phone: 351 916035076. Fax: 351 225577709. E-mail: ricjparaujo{at}yahoo.com

{triangledown} Published ahead of print on 24 August 2009.

Antimicrobial Agents and Chemotherapy, November 2009, p. 4921-4923, Vol. 53, No. 11
0066-4804/09/$08.00+0     doi:10.1128/AAC.00862-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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