This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Enríquez, R.
Right arrow Articles by Vázquez, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Enríquez, R.
Right arrow Articles by Vázquez, J. A.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, February 2009, p. 796-797, Vol. 53, No. 2
0066-4804/09/$08.00+0     doi:10.1128/AAC.00531-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Nalidixic Acid Disk for Laboratory Detection of Ciprofloxacin Resistance in Neisseria meningitidis{triangledown}

Rocío Enríquez, Raquel Abad, Celia Salcedo, and Julio A. Vázquez*

Reference Laboratory for Meningococci-Servicio de Bacteriología, National Centre for Microbiology, National Institute of Health Carlos III, Majadahonda (Madrid), Spain

Received 24 April 2008/ Returned for modification 22 October 2008/ Accepted 8 November 2008

Recently the CLSI recommended a disk diffusion method and breakpoints for meningococci which include breakpoints derived for nalidixic acid which serve as surrogate markers for gyrase A mutations associated with diminished fluoroquinolone susceptibility. This study presents the application of this methodology to a panel of 57 meningococcal strains isolated in Spain that include all levels of susceptibility to ciprofloxacin. In conclusion, the most useful method to predict isolates with gyrA mutations that decrease the activity of fluoroquinolones is the use of 30-µg nalidixic acid disks.

* Corresponding author. Mailing address: Reference Laboratory for Meningococci-Servicio de Bacteriología, National Centre for Microbiology, National Institute of Health Carlos III, Majadahonda (Madrid), Spain. Phone: 34918223617. Fax: 34915097919. E-mail: jvazquez{at}isciii.es

{triangledown} Published ahead of print on 17 November 2008.

Antimicrobial Agents and Chemotherapy, February 2009, p. 796-797, Vol. 53, No. 2
0066-4804/09/$08.00+0     doi:10.1128/AAC.00531-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»