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Antimicrobial Agents and Chemotherapy, March 2009, p. 1231-1234, Vol. 53, No. 3
0066-4804/09/$08.00+0     doi:10.1128/AAC.01173-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Contribution of vraSR and graSR Point Mutations to Vancomycin Resistance in Vancomycin-Intermediate Staphylococcus aureus{triangledown}

Longzhu Cui,1,2* Hui-min Neoh,1,{dagger} Mitsutaka Shoji,2 and Keiichi Hiramatsu1,2

Department of Bacteriology,1 Department of Infection Control Science, Juntendo University, 2-1-1 Hongo, Bunkyo-Ku, Tokyo 113-8421, Japan2

Received 3 September 2008/ Returned for modification 2 November 2008/ Accepted 27 December 2008

We describe here the genetic analysis of a vancomycin-susceptible Staphylococcus aureus (VSSA) strain, Mu50{Omega}, a strain related to vancomycin-intermediate S. aureus (VISA) strain Mu50. Using a combination of Mu50{Omega} whole-genome sequencing and genome engineering, we observed a stepwise evolution of vancomycin resistance from VSSA to VISA after the mutated vraS and graR genes of Mu50 were engineered into Mu50{Omega}.

* Corresponding author. Mailing address: Department of Bacteriology, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-Ku, Tokyo 113-8421, Japan. Phone: (3) 5802-1041. Fax: (3) 5684-7830. E-mail: longzhu{at}juntendo.ac.jp

{triangledown} Published ahead of print on 5 January 2009.

{dagger} Present address: UKM Medical Molecular Biology Institute, University Kebangsaan Malaysia, 56000 Cheras, Kuala Lumpur, Malaysia.

Antimicrobial Agents and Chemotherapy, March 2009, p. 1231-1234, Vol. 53, No. 3
0066-4804/09/$08.00+0     doi:10.1128/AAC.01173-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kato, Y., Suzuki, T., Ida, T., Maebashi, K. (2009). Genetic changes associated with glycopeptide resistance in Staphylococcus aureus: predominance of amino acid substitutions in YvqF/VraSR. J Antimicrob Chemother 0: dkp394v1-dkp394 [Abstract] [Full Text]  
  • Katayama, Y., Murakami-Kuroda, H., Cui, L., Hiramatsu, K. (2009). Selection of Heterogeneous Vancomycin-Intermediate Staphylococcus aureus by Imipenem. Antimicrob. Agents Chemother. 53: 3190-3196 [Abstract] [Full Text]  


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