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Antimicrobial Agents and Chemotherapy, March 2009, p. 876-882, Vol. 53, No. 3
0066-4804/09/$08.00+0     doi:10.1128/AAC.00946-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Naturally Occurring Class A ß-Lactamases from the Burkholderia cepacia Complex {triangledown}

Laurent Poirel,1 José-Manuel Rodriguez-Martinez,1 Patrick Plésiat,2 and Patrice Nordmann1*

Service de Bactériologie-Virologie, INSERM U914, Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris-Sud, K.-Bicêtre,1 Laboratoire de Bactériologie, Faculté de Médecine, Besançon, France2

Received 17 July 2008/ Returned for modification 25 September 2008/ Accepted 24 November 2008

Chromosomally encoded ß-lactamases from the Burkholderia cepacia complex species (formerly Pseudomonas cepacia) were characterized. Cloning and sequencing identified an Ambler class A ß-lactamase (PenB) from B. cenocepacia. It shares 82% amino acid identity with the PenA ß-lactamases previously identified from B. multivorans 249. Its expression was dependent upon a LysR-type regulatory protein. Its narrow-spectrum hydrolysis activity mostly included penicillins but also included expanded-spectrum cephalosporins and aztreonam at lower levels. In that study, Pen-like ß-lactamases (PenC, PenD, PenE, PenF) that shared 63 to 92% identity with PenB from B. cenocepacia were identified from other Burkholderia species. The corresponding ß-lactamase genes might be used as genetic tools for accurate Burkholderia species identification.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.aphp.fr

{triangledown} Published ahead of print on 15 December 2008.

Antimicrobial Agents and Chemotherapy, March 2009, p. 876-882, Vol. 53, No. 3
0066-4804/09/$08.00+0     doi:10.1128/AAC.00946-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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