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Antimicrobial Agents and Chemotherapy, April 2009, p. 1528-1531, Vol. 53, No. 4
0066-4804/09/$08.00+0     doi:10.1128/AAC.00971-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Use of a Surface Plasmon Resonance Method To Investigate Antibiotic and Plasma Protein Interactions {triangledown}

Françoise Banères-Roquet,1,2,3 Maxime Gualtieri,1,2,3 Philippe Villain-Guillot,1,2,3 Martine Pugnière,1,2,3 and Jean-Paul Leonetti1,2,3*

Université Montpellier 1, Centre d'Études d'Agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France,1 CNRS, UMR 5236, CPBS, 4 Bd Henri IV, CS 69033, F-34965 Montpellier, France,2 Université Montpellier 2, CPBS, F-34095 Montpellier, France3

Received 22 July 2008/ Returned for modification 16 December 2008/ Accepted 31 December 2008

The pharmacologic effect of an antibiotic is directly related to its unbound concentration at the site of infection. Most commercial antibiotics have been selected in part for their low propensity to interact with serum proteins. These nonspecific interactions are classically evaluated by measuring the MIC in the presence of serum. As higher-throughput technologies tend to lose information, surface plasmon resonance (SPR) is emerging as an informative medium-throughput technology for hit validation. Here we show that SPR is a useful automatic tool for quantification of the interaction of model antibiotics with serum proteins and that it delivers precise real-time kinetic data on this critical parameter.

* Corresponding author. Mailing address: CNRS-CPBS/UMR 5236, Faculté de Pharamcie, 15 Av Charles Flahault, 34 195 Montpellier Cedex 5, France. Phone: 33 4 67 61 37 46. Fax: 33 4 67 61 37 87. E-mail: Jean-Paul.Leonetti{at}univ-montpl.fr

{triangledown} Published ahead of print on 21 January 2009.

Antimicrobial Agents and Chemotherapy, April 2009, p. 1528-1531, Vol. 53, No. 4
0066-4804/09/$08.00+0     doi:10.1128/AAC.00971-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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