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Antimicrobial Agents and Chemotherapy, July 2009, p. 3140-3142, Vol. 53, No. 7
0066-4804/09/$08.00+0     doi:10.1128/AAC.00213-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Simultaneous Determination of Voriconazole and Posaconazole Concentrations in Human Plasma by High-Performance Liquid Chromatography {triangledown}

Kathrin Kahle, Peter Langmann, Diana Schirmer, Ulrike Lenker, Daniela Keller, Annegret Helle, Hartwig Klinker, and Werner J. Heinz*

Department of Internal Medicine II, Division of Infectious Diseases, University of Wuerzburg Medical Center, Josef-Schneider Str. 2, 97080 Wuerzburg, Germany

Received 16 February 2009/ Returned for modification 21 March 2009/ Accepted 14 April 2009

A simple, sensitive, and selective high-performance liquid chromatographic method for the simultaneous determination of voriconazole and posaconazole concentrations in human plasma was developed and validated. Quantitative recovery following liquid-liquid extraction with diethyl ether was achieved. Linearity ranged from 0.10 to 20.0 µg/ml for voriconazole and from 0.05 to 10.0 µg/ml for posaconazole. The intra- and interday coefficients of variation were less than 8.5%, and the lower limits of quantitation were <0.05 µg/ml.

* Corresponding author. Mailing address: Department of Internal Medicine II, Division of Infectious Diseases, University of Wuerzburg Medical Center, Josef-Schneider Str. 2, 97080 Wuerzburg, Germany. Phone: 49 931 201 36179. Fax: 49 931 201 36485. E-mail: Heinz_W{at}klinik.uni-wuerzburg.de

{triangledown} Published ahead of print on 20 April 2009.

Antimicrobial Agents and Chemotherapy, July 2009, p. 3140-3142, Vol. 53, No. 7
0066-4804/09/$08.00+0     doi:10.1128/AAC.00213-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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