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Antimicrobial Agents and Chemotherapy, July 2009, p. 3162-3165, Vol. 53, No. 7
0066-4804/09/$08.00+0     doi:10.1128/AAC.00093-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Nine-Hospital Study Comparing Broth Microdilution and Etest Method Results for Vancomycin and Daptomycin against Methicillin-Resistant Staphylococcus aureus{triangledown}

Helio S. Sader,* Paul R. Rhomberg, and Ronald N. Jones

JMI Laboratories, North Liberty, Iowa

Received 21 January 2009/ Returned for modification 2 April 2009/ Accepted 20 April 2009

Vancomycin and daptomycin MIC results for 1,800 randomly selected oxacillin (methicillin [meticillin])-resistant Staphylococcus aureus (MRSA) bloodstream isolates from nine U.S. hospitals (collected from 2002 to 2006) were determined by a reference broth microdilution (BMD) method using frozen-form panels with precise incremental dilutions and by the Etest technique. The Etest provided vancomycin and daptomycin MIC results that were consistently higher (0.5 to 1.5 log2 dilution steps) than those provided by the reference BMD method. The dominant MRSA population (91.2% of MRSA isolates) would be categorized as vancomycin nonsusceptible by the MIC results from the Etest method if the susceptibility breakpoint was adjusted downward to ≤1 µg/ml, as suggested by clinical outcome studies.

* Corresponding author. Mailing address: JMI Laboratories, 345 Beaver Kreek Centre, Suite A, North Liberty, IA 52317. Phone: (319) 665-3370. Fax: (319) 665-3371. E-mail: helio-sader{at}jmilabs.com

{triangledown} Published ahead of print on 27 April 2009.

Antimicrobial Agents and Chemotherapy, July 2009, p. 3162-3165, Vol. 53, No. 7
0066-4804/09/$08.00+0     doi:10.1128/AAC.00093-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Howden, B. P., Davies, J. K., Johnson, P. D. R., Stinear, T. P., Grayson, M. L. (2010). Reduced Vancomycin Susceptibility in Staphylococcus aureus, Including Vancomycin-Intermediate and Heterogeneous Vancomycin-Intermediate Strains: Resistance Mechanisms, Laboratory Detection, and Clinical Implications. Clin. Microbiol. Rev. 23: 99-139 [Abstract] [Full Text]  


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