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Antimicrobial Agents and Chemotherapy, August 2009, p. 3501-3504, Vol. 53, No. 8
0066-4804/09/$08.00+0     doi:10.1128/AAC.01620-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Rapid and Reliable Detection of Antimicrobial Peptide Penetration into Gram-Negative Bacteria Based on Fluorescence Quenching{triangledown}

Monica Benincasa, Sabrina Pacor, Renato Gennaro, and Marco Scocchi*

Department of Life Sciences, Via Giorgieri 1, 34127 Trieste, Italy

Received 9 December 2008/ Returned for modification 13 March 2009/ Accepted 14 May 2009

In this paper, we describe a rapid flow cytometry method to identify antimicrobial peptides that are internalized into bacterial cells and differentiate them from those that are membrane active. The method was applied to fluorescently labeled Bac71-35 and polymyxin B, whose mechanisms of action are, respectively, based on cell penetration and on membrane binding and permeabilization. Identification of peptides with the former mechanism is of considerable interest for the intracellular delivery of membrane-impermeant drugs.

* Corresponding author. Mailing address: Department of Life Sciences, University of Trieste, 34127 Trieste, Italy. Phone: 39 040 558 3990. Fax: 39 040 558 3691. E-mail: mscocchi{at}units.it

{triangledown} Published ahead of print on 26 May 2009.

Antimicrobial Agents and Chemotherapy, August 2009, p. 3501-3504, Vol. 53, No. 8
0066-4804/09/$08.00+0     doi:10.1128/AAC.01620-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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