This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Kotsakis, S. D.
Right arrow Articles by Miriagou, V.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kotsakis, S. D.
Right arrow Articles by Miriagou, V.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, August 2009, p. 3520-3523, Vol. 53, No. 8
0066-4804/09/$08.00+0     doi:10.1128/AAC.00219-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Extended-Spectrum Properties of CMY-30, a Val211Gly Mutant of CMY-2 Cephalosporinase{triangledown}

Stathis D. Kotsakis,1 Costas C. Papagiannitsis,1 Eva Tzelepi,1 Leonidas S. Tzouvelekis,2 and Vivi Miriagou1*

Laboratory of Bacteriology, Hellenic Pasteur Institute,1 Department of Microbiology, School of Medicine, University of Athens, Greece2

Received 17 February 2009/ Returned for modification 3 May 2009/ Accepted 17 May 2009

CMY-30, a Val211Gly mutant of CMY-2 cephalosporinase, was derived by mutagenesis. The hydrolytic efficiency of CMY-30 against expanded-spectrum cephalosporins was higher than that of CMY-2 due to increased kcat values. Findings indicate a role of the {Omega} loop residue 211 in determining the substrate specificities of CMYs also corroborated by modeling studies.

* Corresponding author. Mailing address: Laboratory of Bacteriology, Hellenic Pasteur Institute, Vas. Sofias 127, Athens 11521, Greece. Phone: 30-210-6478810. Fax: 30-210-6426323. E-mail: miriagou{at}pasteur.gr

{triangledown} Published ahead of print on 26 May 2009.

Antimicrobial Agents and Chemotherapy, August 2009, p. 3520-3523, Vol. 53, No. 8
0066-4804/09/$08.00+0     doi:10.1128/AAC.00219-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»