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Antimicrobial Agents and Chemotherapy, September 2009, p. 3985-3988, Vol. 53, No. 9
0066-4804/09/$08.00+0     doi:10.1128/AAC.00009-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

In Vitro Trafficking and Efficacy of Core-Shell Nanostructures for Treating Intracellular Salmonella Infections{triangledown}

A. Ranjan,1 N. Pothayee,2 M. N. Seleem,3 N. Sriranganathan,4* R. Kasimanickam,1 M. Makris,4 and J. S. Riffle2

Department of Large Animal Clinical Sciences,1 Macromolecules and Interfaces Institute,2 Institute for Critical Technology and Applied Science,3 Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia4

Received 4 January 2009/ Returned for modification 31 May 2009/ Accepted 6 July 2009

Nanostructures encapsulating gentamicin and having either amphiphilic (N1) or hydrophilic (N2) surfaces were designed. Flow cytometry and confocal microscopy studies demonstrated a higher rate of uptake for amphiphilic surfaces. A majority of N1 were localized in the cytoplasm, whereas N2 colocalized with the endosomes/lysosomes. Colocalization was not observed between nanostructures and intracellular Salmonella bacteria. However, significant in vitro reductions in bacterial counts (0.44 log10) were observed after incubation with N1, suggesting that the surface property of the nanostructure influences intracellular bacterial clearance.

* Corresponding author. Mailing address: Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, 1410 Prices Pork Road, Blacksburg, VA 24061-0342. Phone: (540) 231-7171. Fax: (540) 231-3426. E-mail: nathans{at}vt.edu

{triangledown} Published ahead of print on 13 July 2009.

Antimicrobial Agents and Chemotherapy, September 2009, p. 3985-3988, Vol. 53, No. 9
0066-4804/09/$08.00+0     doi:10.1128/AAC.00009-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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