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Antimicrobial Agents and Chemotherapy, September 2009, p. 4010-4012, Vol. 53, No. 9
0066-4804/09/$08.00+0     doi:10.1128/AAC.00433-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Contribution of dfrA and inhA Mutations to the Detection of Isoniazid-Resistant Mycobacterium tuberculosis Isolates{triangledown}

Yu Min Ho,1 Yong-Jiang Sun,2 Sin-Yew Wong,2 and Ann S. G. Lee1,3*

Division of Medical Sciences, National Cancer Centre, Singapore 169610,1 Department of Infectious Disease, Tan Tock Seng Hospital, Singapore 308433,2 Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 1175973

Received 31 March 2009/ Returned for modification 28 April 2009/ Accepted 25 June 2009

Screening of 127 isoniazid (INH)-resistant Mycobacterium tuberculosis isolates from Singapore for mutations within the dfrA and inhA genes revealed mutations in 0 and 5 (3.9%) isolates respectively, implying that mutations in dfrA do not contribute to the detection of INH-resistant M. tuberculosis and that mutations within inhA are rare. Thirty-seven (29%) of the 127 isolates had no mutations in any of the genes implicated in INH resistance (katG, kasA, and ndh; inhA and ahpC promoters), suggesting that there are new INH targets yet to be discovered.

* Corresponding author. Mailing address: Division of Medical Sciences, National Cancer Centre, Singapore, 11 Hospital Drive, Singapore 169610, Republic of Singapore. Phone: 65 6436-8313. Fax: 65 6372-0161. E-mail: dmslsg{at}nccs.com.sg

{triangledown} Published ahead of print on 6 July 2009.

Antimicrobial Agents and Chemotherapy, September 2009, p. 4010-4012, Vol. 53, No. 9
0066-4804/09/$08.00+0     doi:10.1128/AAC.00433-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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