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Antimicrob Agents Chemother. 1974 October; 6(4): 498-500
Copyright © 1974 American Society for Microbiology. All Rights Reserved.

Rapid, Specific Microbiological Assay for Amikacin (BB-K8)

Department of Pediatrics and Hastings Infectious Disease Laboratory, University of Southern California School of Medicine, and Los Angeles County/University of Southern California Medical Center, Los Angeles, California 90033

ABSTRACT

The emergence of a strain of Providencia stuartii resistant to multiple antibiotics with the exception of amikacin provided a test organism for a microbiological assay for this new semisynthetic aminoglycosidic aminocyclitol. Results can be read at 4 h and are specific for amikacin. The resistance of P. stuartii to all currently used antibiotics allows the utilization of this technique in the presence of other concurrently administered antibiotics and therefore eliminates the need for their inactivation in the assay procedure. The rapidity, specificity, and simplicity of this microbiological assay may provide a technique for routine clinical monitoring of patients on therapeutic regimens and could be utilized by institutions unable to perform the radioimmunoassay or radioassay techniques.