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Antimicrob Agents Chemother. 1976 February; 9(2): 239-246
Copyright © 1976 American Society for Microbiology. All Rights Reserved.

Effect of Bleomycin on Deoxyribonucleic Acid Synthesis in Toluene-Treated Escherichia coli Cells

Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77025

ABSTRACT

The antibiotic bleomycin stimulates deoxyribonucleic acid (DNA) synthesis in toluene-treated Escherichia coli cells. The increase in synthesis is linear with bleomycin concentration. Bleomycin-stimulated DNA synthesis is independent of replication and dependent on DNA polymerase I. Replication is spared as the DNA polymerase I-dependent DNA synthesis increases. Bleomycin does not appear to have any effect on purified E. coli DNA polymerases I or II. Our results suggest that bleomycin causes nicking of the bacterial chromosome with subsequent DNA synthesis catalyzed by DNA polymerase I.