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AAC Accepts, published online ahead of print on 5 May 2008
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AAC.01516-07v1
52/7/2667    most recent
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Antimicrob. Agents Chemother. doi:10.1128/AAC.01516-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Molecular characterization of low level vancomycin resistant Enterococcus faecalis N06-0364 harboring a novel D-Ala-D-Ser gene cluster, vanL

David A. Boyd, Barbara M. Willey, Darlene Fawcett, Nazira Gillani, and Michael R. Mulvey*

National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba; Mount Sinai Hospital and University Health Network, Toronto, Ontario; Rouge Valley Health Centre Centenary Site, Scarborough, Ontario, Canada

* To whom correspondence should be addressed. Email: Michael_mulvey{at}phac-aspc.gc.ca.


  Abstract

Enterococcus faecalis N06-0364 exhibiting a vancomycin MIC of 8 µg/ml was found to harbor a novel D-Ala-D-Ser gene cluster, designated vanL. The vanL gene cluster was similar in organization to the vanC operon but the VanT serine racemase was encoded by two separate genes, vanTmL(membrane-binding) and vanTrL (racemase).




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