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TABLE 2.   Inactivation of HIV-1 by treatment with C31G and SDS


Microbicide and concn (%) during treatment or cell % Cells expressing beta -gal genea No. of cells counted

C31G
   5 × 10-2 (toxic) 0, 0 >5 × 104
   2.5 × 10-2 0, 0 >5 × 104
  1.25 × 10-2 0, 0 >5 × 104
  6.25 × 10-3 19 ± 6.1, 19 ± 6.4 1,080, 805 
   2.5 × 10-3 22 ± 7.4, 29 ± 8.1 1,620, 1,820 
SDS
   5 × 10-2 0, 0 >5 × 104
   2.5 × 10-2 0, 0 >5 × 104
  1.25 × 10-2 24 ± 3.2, 24 ± 10 2,810, 2,190 
  6.25 × 10-3 10 ± 1.7, 15 ± 2.1 2,390, 2,290 
   2.5 × 10-3 9 ± 5.5, 11 ± 3.5 1,940, 1,910 
Mock infected cells 0, 0 >5 × 104
HIV-1-infected cells 17 ± 4.8, 24 ± 5.4 2,680, 1,480

a Data are for duplicate wells. Five random fields of cells were counted in each plate displaying blue cells. Duplicate plates were assayed for each sample; individual numbers are plus or minus the standard deviation within five fields of one plate.




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Clin. Vaccine Immunol. Clin. Microbiol. Rev.
J. Clin. Microbiol. ALL ASM JOURNALS