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TABLE 3. Inhibition of BPV-1 morphologic transformation of C127 cells by SDS treatment of virus

% SDS during treatment^a Final % SDS^b No. of foci/plate^c

Expt 1 (day 12) Expt 2

Day 14 Day 17

0 0 266 255 153

5 5 × 10³ 0 ND ND

5 × 10¹ 5 × 10⁴ 0 ND ND

5 × 10² 5 × 10⁵ 0 0 0

5 × 10³ 5 × 10⁶ 0 271 150

2.5 × 10³ 2.5 × 10⁶ ND 273 162

5 × 10⁴ 5 × 10⁷ ND 229 151

^a Sterile SDS stocks (40 times the treatment concentration) were added to virus aliquots to achieve the treatment concentration.

^b Following virus treatment, treated virus stocks were further diluted 1:1,000 in order to dilute the detergent; the cells in all plates remained viable.

^c In experiment 1, virus and SDS were mixed and incubated at 37°C for 30 min; in experiment 2, virus and SDS were mixed and incubated at 37°C for 10 min. ND, not done. In control plates without BPV-1, no foci appeared.

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