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FIG. 4.
Repetitive microbicide exposure affects the viability of
human vaginal keratinocytes. Primary human vaginal keratinocytes were
exposed to N-9, C31G, or SDS for 2 h. The cells were then washed
once with PBS and cultured under new medium for 24 h. This cycle
of exposure and recovery was repeated two more times. Cell survival was
assessed using MTT assays following the third exposure/recovery cycle.
Cell viability following microbicide exposure is expressed as the
fraction of viable cells relative to the number of mock-exposed cells.
The results illustrated are averages from two experiments in which
triplicate wells for each concentration were assayed.
