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Antimicrobial Agents and Chemotherapy, January 2001, p. 181-186, Vol. 45, No. 1
Department of Medicine, University of Western
Australia, Fremantle Hospital, Fremantle,1 and
Department of Pharmacology, University of Western Australia,
Nedlands,3 Australia, and Tropical
Diseases Research Center, Cho Ray Hospital, Ho Chi Minh
City,2 and Bao Loc Hospital, Bao Loc,
Lam Dong Province,4 Vietnam
Received 28 February 2000/Returned for modification 26 June
2000/Accepted 23 September 2000
To provide novel data relating to the dispositions, effects, and
toxicities of the artemisinin derivatives in severe malaria, we studied
30 Vietnamese adults with slide-positive falciparum malaria treated
with intravenous artesunate. Twelve patients with complications
(severe; group 1) and 8 patients without complications but requiring
parenteral therapy (moderately severe; group 2) received 120 mg of
artesunate by injection, and 10 patients with moderately severe
complications (group 3) were given 240 mg by infusion. Serial
concentrations of artesunate and its active metabolite dihydroartemisinin in plasma were measured by high-performance liquid
chromatography. The time to 50% parasite clearance (PCT50) was determined from serial parasite densities. Full clinical (including neurological) assessments were performed at least daily. In
noncompartmental pharmacokinetic analyses, group mean artesunate
half-lives (t1/2) were short (range, 2.3 to 4.3 min). The dihydroartemisinin t1/2 (range, 40 to
64 min), clearance (range, 0.73 to 1.01 liters/h/kg), and volume of
distribution (range, 0.77 to 1.01 liters/kg) were also similar both
across the three patient groups (P > 0.1) and to
previously reported values for patients with uncomplicated malaria.
Parasite clearance was prompt (group median PCT50 range 6 to 9 h) and clinical recovery was complete under all three
regimens. These data indicate that the pharmacokinetics of artesunate
and dihydroartemisinin are not influenced by the severity of malaria. Since the pharmacokinetic parameters for both artesunate and
dihydroartemisinin were similar regardless of whether injection or
infusion was used, artesunate can be considered a prodrug that is
converted stoichiometrically to dhydroartemisinin. Conventional doses
of artesunate are safe and effective when given to patients with
complications of falciparum malaria.
Artemisinin and its semisynthetic
derivatives are potent, well-tolerated compounds that are used as
first-line antimalarial therapy in many tropical countries (1,
16, 22, 38). The derivative with the widest potential
application is artesunate (ARTS), a water-soluble prodrug that can be
administered parenterally as well as by mouth and per rectum. A number
of studies have demonstrated the efficacy of ARTS in uncomplicated
(17, 19, 24, 26, 27, 28), severe (20, 21),
and multidrug-resistant (11, 25, 34) falciparum malaria.
Since ARTS clears parasites more rapidly than quinine and may improve
survival (1, 16, 22, 24, 38), intravenous (i.v.) ARTS has
become an alternative to i.v. quinine for the treatment of severe
malaria in many countries.
The pharmacokinetics and pharmacodynamics of ARTS and other artemisinin
derivatives have been assessed in patients with uncomplicated malaria
(3, 6, 7, 9), but there have been no equivalent studies
with patients with severe Plasmodium falciparum infections. The dosing regimens used for patients with complications remain empirical and do not differ significantly from those used for patients
with milder malaria (16, 22, 38). Although neurotoxicity has yet to be demonstrated objectively in humans receiving conventional doses of artemisinin drugs, residual concerns remain (10, 12, 18,
23, 30, 35). Since neurological dysfunction leading to coma in
its most severe form is a serious complication of falciparum malaria
(37), there is an added need for data relating to the disposition and effects of ARTS in severe malaria.
We have studied the pharmacokinetics, pharmacodynamics, and toxicities
of ARTS and its equipotent, longer-acting active metabolite dihydroartemisinin (DHA) in patients with severe malaria given a
conventional dose of ARTS by i.v. injection. Since i.v. ARTS is
converted rapidly to DHA and the half-life
(t1/2) of DHA itself is short compared to the
recommended interval between doses, administration of ARTS by i.v.
infusion has been suggested as a way of increasing the duration of
parasite exposure to therapeutic drug concentrations as well as
limiting the potential toxicity of high peak levels postinjection
(4). In a second study, therefore, we examined the
disposition, antimalarial efficacy, and toxicity of ARTS given by
injection or 4-h infusion to patients with moderately severe falciparum malaria.
Patients.
We studied 30 Vietnamese farmers or rural laborers
with falciparum malaria requiring parenteral therapy who were admitted to Cho Ray Hospital, Ho Chi Minh City, or to Bao Loc Hospital, Bao Loc,
Lam Dong Province. After confirmation of the diagnosis by microscopy, a
complete clinical assessment including drug history was performed.
Patients were classified as having severe malaria (37) if
they were (i) in a coma (Glasgow coma score [GCS], <11), (ii)
jaundiced (serum bilirubin level, >50 µmol/liter; serum aspartate transaminase [AST] level, more than twice the upper limit of the reference range), (iii) in renal failure (serum creatinine level, >250
µmol/liter after rehydration), (iv) anemic (venous hematocrit level,
<15%), or (v) hyperparasitemic (>250,000 asexual forms/µl of whole
blood). Patients were considered as having moderately severe malaria if
they did not have any of the features described above but could not be
given oral therapy because of nausea, vomiting, or confusion.
0066-4804/01/$04.00+0 DOI: 10.1128/AAC.45.1.181-186.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Pharmacokinetics and Pharmacodynamics of
Intravenous Artesunate in Severe Falciparum Malaria
ABSTRACT
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Abstract
Introduction
Materials and Methods
Results
Discussion
References
INTRODUCTION
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Abstract
Introduction
Materials and Methods
Results
Discussion
References
MATERIALS AND METHODS
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Abstract
Introduction
Materials and Methods
Results
Discussion
References
Study design and procedures. The study was performed in two phases. In phase 1, 12 patients with severe falciparum malaria (group 1) were given 120 mg of ARTS (Guilin No. 2 Pharmaceutical Factory, Guangxi, China) diluted in 10 ml of 5% (wt/vol) dextrose and administered as an i.v. bolus over 2 min. Venous blood samples were obtained from the arm opposite that used for drug administration at 0 (predosing), 5, 7, 9, 12, 15, 20, 30, 45, 60, 90, 120, 180, and 240 min. Further 60-mg doses of i.v. ARTS were scheduled at 24 and 48 h, but additional doses could be given at the discretion of the attending physician. Mefloquine (15 mg/kg of body weight) was given at 72 h, or if this was precluded by the clinical state of the patient, daily ARTS administration was continued until oral mefloquine could be tolerated.
In phase 2, patients with moderately severe malaria were randomized to receive ARTS by either i.v. injection or infusion. Because infusions of ARTS had not been evaluated previously, we used a total initial dose of approximately 4 mg/kg/day, which is at the upper end of the range usually recommended for falciparum malaria (1, 16). Eight patients (group 2) were given 120 mg of ARTS i.v., and the same sampling protocol used for group 1 was followed. A second dose of 120 mg of ARTS was given i.v. immediately after the last (240 min) blood sample had been taken, but no other samples were drawn subsequently. Ten other patients (group 3) were given the same total dose of ARTS given to those in group 2 (240 mg), but the dose was diluted in 50 ml of 5% (wt/vol) dextrose and was given as an i.v. infusion over 4 h with a motor-driven infusion pump. For these patients, venous blood was drawn at 0, 5, 7, 9, 12, 15, 20, 30, 45, 60, 90, 120, 180, and 240 min during the infusion and then at 5, 7, 9, 12, 15, 20, 30, 45, 60, 90, and 120 min after the infusion had been stopped. Patients in phase 2 were scheduled to receive further doses of ARTS at 60 mg i.v. at 24 h and a single dose of mefloquine on the second or third day of hospitalization. A complete physical examination, including full neurological assessment and determination of GCS, was performed at least daily for each patient, and the results were recorded on standard forms. All blood samples were collected in fluoride-oxalate tubes and were chilled immediately to prevent ARTS degradation by plasma esterases. Samples were centrifuged within 30 min to minimize hemolysis, and the separated plasma was stored below
20°C until analyzed. Thick and thin blood
films were prepared at 0, 0.5, 1, 2, 3, 4, 6, 8, 12, 18, and 24 h
and every 6 h thereafter until parasite clearance. Oral
temperature was measured every 4 h. Patients were discharged when
they were afebrile and aparasitemic.
Pharmacokinetic and pharmacodynamic analyses.
Plasma samples
were assayed by a validated high-performance liquid chromatography
(HPLC) method (2, 6). ARTS has previously been shown to be
stable in 5% (wt/vol) dextrose at 30°C, with <10% being hydrolyzed
in 7 h, while the stabilities of ARTS (780 and 4,560 nmol/liter)
and DHA (1,060 and 6,160 nmol/liter) in plasma have been assessed for
up to 12 months at 25°C and found to be within ±7.6% of those for
replicate samples stored for equivalent lengths of time at 80°C
(2, 4). As reported previously (3, 4, 6, 7),
the batch purity of the i.v. ARTS used was checked by HPLC and was
found to be equivalent to that stated by the manufacturer. Conventional
pharmacokinetic parameters (area under the plasma concentration-time
curve [AUC] from time zero to infinity [AUC0-
i; with
extrapolation to 
as
Clast/
z, where
Clast is the final measured concentration and
z is the terminal elimination rate
constant], t1/2, clearance [CL], volume of
distribution at pseudo-distribution equilibrium
[Vz], maximum concentration in plasma
[Cmax], and the time to
Cmax [Tmax]) were
determined from the plasma concentration-time data by noncompartmental analysis (36) and by assuming complete bioconversion of
ARTS to DHA.
Statistical analysis. Differences between means were analyzed by Student's t test or one-way analysis of variance (ANOVA), as appropriate, with the least-significant-difference post hoc test (SPSS for Windows; SPSS Inc., Chicago, Ill.). For variables that were not normally distributed by the Kolmogorov-Smirnov one-sample test, Wilcoxon-Mann-Whitney and Kruskall-Wallis tests were used. The Spearman rank correlation was used to evaluate relationships between pharmacokinetic and pharmacodynamic parameters. Data are reported as means ± standard deviations (SDs). Two-tailed significance levels were used.
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RESULTS |
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Abstract
Introduction
Materials and Methods
Results
Discussion
References
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Clinical course.
Details for the patients in the three groups
at presentation are given in Table 1.
Consistent with the criteria used for classification, the patients with
severe malaria (group 1) had significantly greater serum creatinine,
bilirubin, and AST concentrations than those in the other two groups
(P < 0.01 by ANOVA in each case). Five group 1 patients had a GCS <11. All other demographic, clinical, and
laboratory measurements for the three groups were similar (P > 0.1).
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Pharmacokinetic analysis. (i) ARTS.
Pharmacokinetic data for
ARTS are summarized in Fig. 1 and Table
2. ARTS had a short mean
t1/2 in each of the three groups, the shortest
being in the severely ill patients (group 1). These patients also had a
mean value of Vz that was significantly lower than those for the other two groups (Table 2). Mean CL values also
tended to be lowest for patients in group 1 and highest for the
patients with moderately severe malaria who received ARTS by infusion
(group 3).
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(ii) DHA. Apart from AUC and Cmax, the pharmacokinetics of DHA after i.v. ARTS administration were similar in the three groups (Table 2). The mean AUC for group 3 was consistent with the dose of ARTS given, approximately double those for the other two groups, while the mean Cmax for group 3 (at the end of the infusion period) was significantly lower than those for the other two groups. Consistent with the t1/2 for each compound, steady-state concentrations of ARTS were achieved early on during the infusion in group 3 patients, whereas for DHA, steady state was reached late in the 240-min infusion period (Fig. 1).
Pharmacodynamic analysis.
Four group 1 patients and two group
2 patients received additional unscheduled doses of i.v. ARTS within
the first 24 h. Parasite and fever clearance data for these
patients were therefore excluded from the pharmacodynamic analysis
(Table 2). There were no between-group differences in FCT (P = 0.7). However, the six evaluable group 1 patients had the
greatest PCT50s (P < 0.05 versus the
values for groups 2 and 3). This was not a function of the initial
parasitemia because there was no significant correlation with
PCT50 (rs = 0.26; n = 24;
P = 0.22). To determine whether the between-group differences
in PCT50s resulted from the relatively greater severity of
illness in group 1 patients or from the higher doses of i.v. ARTS given
to patients in groups 2 and 3 during the first 24 h of treatment,
we investigated the pharmacodynamics of ARTS and DHA by assessing
correlations between drug AUC values and PCT50s. Because
patients in group 2 had received two doses of 120 mg of ARTS 4 h
apart, we doubled the AUC values for both DHA and DHA plus ARTS after
the first injection for these subjects. There was a nonsignificant
but borderline inverse correlation between PCT50 and AUC
for DHA in the 24 patients (rs = 0.40; P = 0.06) (Fig. 2). Because ARTS and
DHA both possess the endoperoxide moiety thought to be responsible for
the antimalarial activities of the artemisinin drugs (22),
ARTS may contribute to parasite clearance, despite its short
t1/2. The correlation between PCT50
and total AUC for ARTS and DHA combined was similar to that between
PCT50 and the AUC for DHA alone (rs = 0.40; P = 0.07) (Fig. 2). In both cases (AUC for DHA and
AUC for DHA and ARTS combined), an AUC value of <10 µmol · h/liter was especially likely to be associated with a
PCT50 >10 h.
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DISCUSSION |
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Introduction
Materials and Methods
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Discussion
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The present studies were generated primarily by the concern that, analogous to quinine (15, 39), the complications of falciparum malaria could be associated with clinically significant changes in the pharmacokinetics of ARTS and DHA. Rational parenteral quinine regimens have been developed specifically for severely ill patients (37). By contrast, the relative paucity of pharmacokinetic data for ARTS, even in patients without complications, means that dosing regimens for severe malaria remain empirical. As well as providing novel pharmacokinetic data for ARTS and DHA in this situation, we wished to assess whether i.v. infusion of ARTS both achieved the predicted plasma DHA concentrations and cleared the parasitemia at least as effectively as ARTS given in equivalent doses by i.v. injection. Although the risk of toxicity associated with the artemisinin derivatives appears to be very low, the present study provided further opportunities to monitor the side effects of ARTS in a controlled situation.
Apart from a case study of a Caucasian patient who received i.v. ARTS by injection (13), our data are the first relating to the disposition and effects of an artemisinin derivative in severe falciparum malaria. The t1/2 of ARTS has been estimated to be between 2 and 5 min in a variety of previous studies in both healthy volunteers (40, 41) and patients with uncomplicated malaria (3, 6, 9). Mean values for our three groups of patients and that for our Caucasian patient (13) lie within this range. Although there was a significantly shorter t1/2 in our 12 patients with severe malaria compared with those in the 18 patients who had moderately severe malaria in the presence of a lower CL and Vz, these differences are unlikely to be of clinical significance since ARTS is rapidly metabolized and can thus be considered a prodrug. The pharmacokinetics of its longer-acting metabolite, DHA, were comparable across the three groups, apart from parameters relating to the double-dose and 4-h administration time for group 3 patients. Mean values for DHA elimination t1/2, CL, and Vz in the three groups were comparable to those after i.v. ARTS administration for healthy controls, patients with uncomplicated malaria, and the one patient with severe malaria reported previously (3, 6, 7, 8, 9, 13, 40, 41). We conclude, therefore, that the pharmacokinetics of ARTS and DHA are not influenced by the severity of the malaria.
A further indication that ARTS and DHA pharmacokinetics are independent
of infection severity is provided by Fig.
3. Mean concentration profiles for both
ARTS and DHA in plasma for group 3 patients during and after ARTS
infusion are shown in Fig. 3, together with theoretical plots derived
from pharmacokinetic data for patients with uncomplicated falciparum
and vivax malaria given ARTS by i.v. injection (4). The
latter plots have been generated by using an open one-compartment model
under the assumption that there is complete metabolism of ARTS to DHA.
The theoretical and actual curves are similar, apart from some
divergence at low plasma ARTS concentrations postinfusion. This
divergence reflects the fact that the ARTS concentrations in some group
3 patients were below the assay detection limit (250 nmol/liter) at
these times and were not used in the calculation of mean values.
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In a previous study with an isolated perfused rat liver model (5), we found that DHA had a high, concentration-dependent hepatic extraction ratio that was reduced by 20 to 30% in rodents with Plasmodium berghei malaria. Elimination of DHA-glucuronide, the principal biliary metabolite, was reduced by 40 to 50% in rats with malaria, probably as a result of decreased intrinsic metabolic clearance. By contrast, the present data suggest indirectly that hepatic dysfunction complicating falciparum malaria does not alter DHA clearance significantly. This may reflect the fact that glucuronidation is a high-capacity reaction occurring in many tissues (32) and may not be the only metabolic pathway for DHA in humans (31).
Parasite clearance was prompt in all three groups, with PCT50s that did not exceed 20 h. However, the PCT50 for the severely ill patients who received a lower dose of ARTS in the first 24 h was significantly longer than those for patients in the other two groups. When the data for all three groups were considered together, there was a trend to a shorter PCT50 for patients with greater AUC values for DHA both alone and in combination with ARTS. Although the PCT50s for the six group 1 and 2 patients given additional unscheduled doses of i.v. ARTS were close to the group medians (data not shown), our preliminary pharmacodynamic data suggest that it may be appropriate to give more than 120 mg of ARTS in the first 24 h of treatment to patients with at least moderately severe falciparum malaria. This might take the form of a second i.v. injection of 60 to 120 mg of ARTS at 4 to 8 h or the use of a high-dose ARTS infusion, as was done for group 3 patients. The precise mechanism of action of the artemisinin derivatives is not yet fully understood. Nevertheless, our data for the group 3 patients suggest that their rapidity of action does not depend on peak concentrations in plasma.
Other than those that are attributable to falciparum malaria per se (37) or that occur after parasite clearance, especially in the context of mefloquine administration (33), neurological symptoms and signs were not observed in any of our patients. Although there is evidence from in vitro and animal studies that artemisinin derivatives are neurotoxic (10, 12, 23, 35), this has not been documented in controlled, prospective studies with humans (1, 16, 22, 38). In recent single case reports of an association between treatment with an artemisinin derivative and cerebellar dysfunction (30) and tremor (18), it is difficult to know whether the neurological features were due to artemisinin, malaria itself, or other contemporaneous medications or disease processes (14). There have been no reports of similar features in severely ill patients treated with an artemisinin drug.
Severe malaria remains a condition still associated with unacceptably high rates of morbidity and mortality (37). Our results extend current knowledge of the disposition and effects of artemisinin derivatives to patients with complications of falciparum malaria and confirm that ARTS given at doses of 2 to 4 mg/kg/day is both safe and effective when given as an i.v. bolus or as an infusion, including to those presenting in a coma. Consistent with the results of previous in vitro and in vivo studies (1, 16, 22, 38), our results imply that rapid initial parasite clearance after ARTS administration can be achieved after a relatively brief exposure of parasitized erythrocytes to drug and/or active metabolite.
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ACKNOWLEDGMENTS |
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We thank Trinh Kim Anh, Nguyen Phuc Tien, Truong Xuan Mai, and Vuong Van Chon, Cho Ray Hospital, and Vo Thanh Chien, Vu Nam Bien, Dang Thi Vinh Thuan, and the staff of the Malaria and Biochemistry & Hematology Departments, Bao Loc Hospital, for facilitating the conduct of this study.
This study was supported by a project grant from the National Health and Medical Research Council (NHMRC) of Australia (to T.M.E.D. and K.F.I.). K.T.B. was a recipient of an NHMRC Dora Lush (Biomedical) Scholarship.
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FOOTNOTES |
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* Corresponding author. Mailing address: Department of Medicine, University of Western Australia, Fremantle Hospital, P.O. Box 480, Fremantle 6959, Australia. Phone: (618) 9431 3229. Fax: (618) 9431 2977. E-mail: tdavis{at}cyllene.uwa.edu.au.
Present address: School of Pharmacy, Curtin University of
Technology, Bentley, Western Australia, Australia.
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REFERENCES |
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Abstract
Introduction
Materials and Methods
Results
Discussion
References
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Antimicrobial Agents and Chemotherapy, January 2001, p. 181-186, Vol. 45, No. 1
0066-4804/01/$04.00+0 DOI: 10.1128/AAC.45.1.181-186.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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