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Antimicrobial Agents and Chemotherapy, April 2004, p. 1435-1436, Vol. 48, No. 4
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.4.1435-1436.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

LETTER TO THE EDITOR

Antimalarial and Antileishmanial Activities of Aroyl-Pyrrolyl-Hydroxyamides, a New Class of Histone Deacetylase Inhibitors


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LETTER
 
Members of the genus Leishmania are parasitic protozoans that infect about two million people per annum (5), and they are emerging as serious opportunistic infective agents in human immunodeficiency virus-infected patients (4). Malaria parasites are responsible for 1.5 to 2.7 million deaths annually, primarily in Africa (10). The effort to find new antimalarial agents is still a high priority given the increasing malaria emergency largely due to multidrug-resistant Plasmodium falciparum strains. The histones of P. falciparum have recently been proposed as targets for drug treatment of blood stage parasites (6). They also play an important role in chromatin remodeling in trypanosomatids, which include Leishmania species and trypanosomes (3).

Apicidin, a cyclic tetrapeptide isolated from Fusarium spp., was reported to block the in vitro development of apicomplexan parasites by inhibiting parasite (including Plasmodium species) histone deacetylase (HDAC) (6). Another HDAC inhibitor, suberoyl bishydroxamic acid, showed an in vivo cytostatic effect against the acute murine malaria Plasmodium berghei, and one round of treatment with the compound failed to select for resistant mutations (1).

Recently, Mai et al. reported a novel series of hydroxamate compounds, namely, 3-(4-aroyl-1H-pyrrol-2-yl)-N-hydroxy-2-propenamides, acting as HDAC inhibitors in the range of low micromolar-submicromolar concentrations (7, 8). The aim of the present study was to investigate the in vitro antimalarial and antileishmanial activities of lead compound 1 and some analogues (compounds 2 to 10) to identify potential chemical tools with selective toxicity for protozoa.

The antimalarial activity of compounds 1 to 10 (Table 1) was determined in vitro for chloroquine-sensitive (CQS) (D6, Sierra Leone) and chloroquine-resistant (CQR) (W2, Indochina) strains of P. falciparum. Growth of cultures of P. falciparum was determined by a parasite lactate dehydrogenase assay using Malstat reagent (9). Chloroquine was used as the positive control, while dimethyl sulfoxide was tested as the negative control. Suberoylanilide hydroxamic acid (SAHA) and trichostatin A (TSA), two well-known HDAC inhibitors, were also tested. Antileishmanial activity of compounds 1 to 10 (Table 1) was tested on a transgenic cell line of Leishmania donovani promastigotes expressing firefly luciferase (assay with Steady Glo reagent; Promega, Madison, Wis.) obtained from Dr. Rafael Balana-Fouce, University of Leon, Leon, Spain. Pentamidine was tested as a reference drug together with SAHA and TSA. All the compounds were simultaneously tested for cytotoxicity on Vero (monkey kidney fibroblast) cells by a Neutral Red assay (2).


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  		TABLE 1. Antimalarial and antileishmanial activities of compounds 1 to 10

Among compounds 1 to 10, only compound 7 showed antimalarial activity against P. falciparum strains; however, its 50% inhibitor concentration (IC50) values were 22- to 100-fold higher than those of chloroquine and 4.8- to 8.5-fold and 33- to 93-fold higher than those of SAHA and TSA, respectively. Compounds 1 to 4 showed little Plasmodium inhibition activity (Table 1). This biological behavior of compounds 1 to 10 resembles their corresponding anti-HDAC effect against maize HD2 (compound 7, IC50 = 0.1 µM; compounds 1 to 4, IC50 = 2 to 4 µM; compounds 5, 6, and 8 to 10, low-level activity or totally inactivity) (7, 8), thus confirming an inhibiting action of compound 7 and, to a lesser extent, of compounds 1 to 4 on parasite HDAC enzymes.

Surprisingly, the majority of compounds 1 to 10 were found endowed with interesting anti-Leishmania activity (in this case, activity not directly related to their anti-HD2 action) (Table 1). Compounds 2 and 3, the most potent of the series, were as active as pentamidine, slightly less potent than TSA, and >10-fold more potent than SAHA. Interestingly, compounds 2 and 3 were less cytotoxic than the reference drugs. Further studies to elucidate the mechanism of anti-Leishmania activity of such derivatives are in progress.


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ACKNOWLEDGMENTS
 
The work was supported by "Progetto Finalizzato Ministero della Salute 2002" and AIRC 2003 grants (A.M.), by Centers for Disease Control Cooperative Agreement grants U50-CCU418839 (EIDs) and UR3-CCU418652 (malaria), and by U.S. Department of Agriculture Cooperative Agreement grant 58-6408-2-0009 to the National Center for Natural Products Research (L.A.W. and B.L.T.).


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  7. Mai, A., S. Massa, R. Ragno, I. Cerbara, F. Jesacher, P. Loidl, and G. Brosch. 2003. 3-(4-Aroyl-1-methyl-1H-2-pyrrolyl)-N-hydroxy-2-alkylamides as a new class of synthetic histone deacetylase inhibitors. 1. Design, synthesis, biological evaluation, and binding mode studies performed through three different docking procedures. J. Med. Chem. 46:512-524.[CrossRef][Medline]
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  10. Wirth, D. F. 1999. Malaria: a third world disease in need of first world drug development. Annu. Rep. Med. Chem. 34:349-358.
Antonello Mai*
Ilaria Cerbara
Sergio Valente
Dipartimento Studi Farmaceutici
Università degli Studi di Roma "La Sapienza"
P.le Aldo Moro 5
00185 Rome, Italy

Silvio Massa
Dipartimento Farmaco Chimico Tecnologico
Università degli Studi di Siena
53100 Siena, Italy

Larry A. Walker
Babu L. Tekwani{dagger}
National Center for Natural Products Research   and Department of Pharmacology
School of Pharmacy
University of Mississippi
University, Mississippi 38677-1848

* Phone: 39-649913392, Fax: 39-396491491, E-mail: antonello.mai{at}uniroma1.it

{dagger} Phone: (662) 915-7882, Fax: (662) 915-7062, E-mail: btekwani{at}olemiss.edu.

Antimicrobial Agents and Chemotherapy, April 2004, p. 1435-1436, Vol. 48, No. 4
0066-4804/04/$08.00+0     DOI: 10.1128/AAC.48.4.1435-1436.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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  • Andrews, K. T., Tran, T. N., Lucke, A. J., Kahnberg, P., Le, G. T., Boyle, G. M., Gardiner, D. L., Skinner-Adams, T. S., Fairlie, D. P. (2008). Potent Antimalarial Activity of Histone Deacetylase Inhibitor Analogues. Antimicrob. Agents Chemother. 52: 1454-1461 [Abstract] [Full Text]  
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