Skip to main page content

• HOME
• Current Issue
• Archive
• Alerts
• About ASM
• Contact us
• Tech Support
• Journals.ASM.org

This Article Abstract Full Text (PDF) Supplemental material Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liu, T. T. Articles by Rogers, P. D. Search for Related Content PubMed PubMed Citation Articles by Liu, T. T. Articles by Rogers, P. D.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, June 2005, p. 2226-2236, Vol. 49, No. 6
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.6.2226-2236.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Genome-Wide Expression Profiling of the Response to Azole, Polyene, Echinocandin, and Pyrimidine Antifungal Agents in Candida albicans

Teresa T. Liu,^1,2 Robin E. B. Lee,³ Katherine S. Barker,^1,2 Richard E. Lee,³ Lai Wei,⁴ Ramin Homayouni,⁴ and P. David Rogers^1,2,3,5*

Departments of Pharmacy,¹ Pharmaceutical Sciences,³ College of Pharmacy, and Departments of Pediatrics,⁵ Neurology, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163,⁴ Children's Foundation Research Center at Le Bonheur Children's Medical Center, Memphis, Tennessee 38103²

Received 18 November 2004/ Returned for modification 22 December 2004/ Accepted 14 February 2005

Top Abstract Introduction Materials and Methods Results Discussion References

 
Antifungal agents exert their activity through a variety of mechanisms, some of which are poorly understood. We examined changes in the gene expression profile of Candida albicans following exposure to representatives of the four currently available classes of antifungal agents used in the treatment of systemic fungal infections. Ketoconazole exposure increased expression of genes involved in lipid, fatty acid, and sterol metabolism, including NCP1, MCR1, CYB5, ERG2, ERG3, ERG10, ERG25, ERG251, and that encoding the azole target, ERG11. Ketoconazole also increased expression of several genes associated with azole resistance, including CDR1, CDR2, IFD4, DDR48, and RTA3. Amphotericin B produced changes in the expression of genes involved in small-molecule transport (ENA21), and in cell stress (YHB1, CTA1, AOX1, and SOD2). Also observed was decreased expression of genes involved in ergosterol biosynthesis, including ERG3 and ERG11. Caspofungin produced changes in expression of genes encoding cell wall maintenance proteins, including the ß-1,3-glucan synthase subunit GSL22, as well as PHR1, ECM21, ECM33, and FEN12. Flucytosine increased the expression of proteins involved in purine and pyrimidine biosynthesis, including YNK1, FUR1, and that encoding its target, CDC21. Real-time reverse transcription-PCR was used to confirm microarray results. Genes responding similarly to two or more drugs were also identified. These data shed new light on the effects of these classes of antifungal agents on C. albicans.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Candida albicans is the most common human fungal pathogen and is the fourth leading cause of bloodstream infections in the United States (6, 14, 24). Currently only four antifungal drug classes are available for the management of systemic infections due to Candida species. Recently we examined changes in the genome-wide expression profile of Saccharomyces cerevisiae in response to representatives of the polyene, pyrimidine, azole, and echinocandin antifungal agents in an effort to identify class-specific and mechanism-independent changes in gene expression (1). In the present study, we extend this analysis to the pathogenic fungus C. albicans. By using the same representative drugs and similar growth conditions as in our previous study, we are able to show similarities and differences in the responses to these antifungal agents between S. cerevisiae and C. albicans. Gene expression profiling experiments revealed drug-specific responses consistent with their mechanisms of action, responses indicative of other pathways that may be affected by these agents, and responses that reflect known and potential mechanisms of resistance to these antifungal drugs.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Antifungal agents. Ketoconazole (KTZ) and flucytosine (5-FC) were obtained from Sigma (St. Louis, MO). Amphotericin B (AMB) was obtained from ICN Biomedicals (Aurora, OH). The commercially available preparation of caspofungin (CPF) acetate for injection (Cancidas) was used. Stock solutions of various concentrations were made in dimethyl sulfoxide (DMSO; Sigma).

C. albicans strains and media. C. albicans strain SC5314, obtained from the American Type Culture Collection (Manassas, VA), was used for microarray experiments. Synthetic dextrose medium, containing 0.67% (wt/vol) yeast nitrogen base without amino acids and 2% (wt/vol) dextrose, was used to grow strain SC5314. The medium was buffered with 0.165 M morpholinepropanesulfonic acid (MOPS; Sigma), and the pH was adjusted to 7.0 with NaOH.

IC₅₀ determinations. To ensure consistent results, 50% inhibitory concentrations (IC₅₀s) were determined under the same environmental conditions used in subsequent microarray analysis, including incubation times, culture volume (100 ml), flask size (250-ml Corning 430281 roller bottle), temperature (30°C), and shaking speed (225 rpm). All measurements of turbidity were read at 600 nm on a Beckman DU530 spectrophotometer. Cultures with an A₆₀₀ exceeding 1.0 were diluted 1:10 in water for measurement. One hundred milliliters of Synthetic dextrose medium was inoculated from a fresh, saturated culture of C. albicans to an initial A₆₀₀ of 0.005 and grown at 30°C until the A₆₀₀ reached 1.0 (approximately 12 h). The culture was then diluted to an A₆₀₀ of 0.1 and allowed to recover from stationary phase at 30°C/225 rpm until an A₆₀₀ of 0.2 was reached (approximately 2 h). Antifungal drug was then added according to the desired concentration from a 10-mg/ml drug stock in DMSO, and an equal amount of DMSO was added to the control culture. DMSO concentrations were below 0.05% and did not affect the growth curves of control cultures. The cultures were grown for 6 h, and the A₆₀₀ was measured at both 3 and 6 h. IC₅₀s were calculated at 6 h, in reference to the control culture. Three rounds of experiments were conducted from which the average IC₅₀ was calculated. In the first experiment, a broad range of six twofold serial dilutions were tested based on the MIC. A narrow range of three drug concentrations was then tested in the second and third rounds (KTZ, 6.25 to 25 µg/ml; AMB, 0.025 to 0.1 µg/ml; CPF, 0.005 to 0.02 µg/ml; 5-FC, 0.05 to 0.2 µg/ml). Cells used for subsequent microarray analysis were grown at the average IC₅₀ to ensure 50% growth inhibition.

Drug exposure for microarray experiments. C. albicans was grown as described in the IC₅₀ section above. For a single microarray experiment, a total of six 100-ml cultures were prepared, three with an antifungal drug added at a concentration equivalent to the IC₅₀ (KTZ, 19.13 µg/ml; AMB, 0.029 µg/ml; CPF, 0.0075 µg/ml; 5-FC, 0.098 µg/ml) and three control cultures treated with an appropriate amount of DMSO. Three hours after drug addition, the A₆₀₀ was measured and two control cultures and two drug-treated cultures were harvested by centrifugation at 3,600 x g at 4°C for 5 min. The medium was completely removed by aspiration, and the cell pellets were flash frozen on dry ice and stored at –80°C until RNA preparation. The remaining control and drug-treated cultures were allowed to grow for an additional 3 h, at which point the A₆₀₀ was measured and the percent growth calculated.

RNA preparation. RNA was isolated using the hot phenol method (30). Frozen cells were resuspended in 12 ml of AE buffer (50 mM sodium acetate [pH 5.2], 10 mM EDTA) at room temperature, after which 800 µl of 25% sodium dodecyl sulfate and 12 ml of acid phenol (Fisher Scientific, Houston, TX) were added. The cell lysate was then incubated for 10 min at 65°C with vortexing each minute, cooled on ice for 5 min, and subjected to centrifugation for 15 min at 11,952 x g. Supernatants were transferred to new tubes containing 15 ml of chloroform, mixed, and subjected to centrifugation at 200 x g for 10 min. RNA was precipitated from the resulting aqueous layer by transferring that portion to new tubes containing 1 volume of isopropanol and 0.1 volume of 2 M sodium acetate (pH 5.0), mixing well, and subjecting the mixture to centrifugation at 17,211 x g for 35 min at 4°C. The supernatants were removed, the pellet was resuspended in 10 ml of 70% ethanol, and the RNA was collected by centrifugation at 17,211 x g for 20 min at 4°C. Supernatants were again removed, and the RNA pellet was resuspended in diethyl pyrocarbonate-treated water. Optical densities were measured at 260 and 280 nm, and integrity of RNA was visualized by subjecting 2 to 5 µl of the sample to electrophoresis through a 1% agarose-MOPS gel.

Microarray hybridization. The C. albicans microarrays used in this study were manufactured by Eurogentec SA (Ivoz-Ramet, Belgium) in collaboration with the European Galar Fungail Consortium (www.pasteur.fr/recherche/unites/Galar_Fungail/). Two independent sets of RNA from control and drug-treated cells (biological replicates) were used in these studies to prepare two independent cDNA probe sets. Ten micrograms of total RNA sample was added to a mixture of 1 pmol of T20VN and oligo(dT) (18- to 21-mer) primer mix (C. albicans-specific primer mix plus) (Eurogentec, Ivoz-Ramet, Belgium); 0.5 mM each dATP, dGTP, and TTP; 20.5 µM dCTP; 37.5 µM Cy3- or Cy5-dCTP (NEN Life Sciences; Boston, MA); and 10 mM dithiothreitol in a buffer containing 50 mM Tris-HCl (pH 8.3), 75 mM KCl, and 3 mM MgCl₂. The reaction mixture was denatured at 65°C for 5 min and incubated at 42°C for 5 min, after which 1 µl of RNasin (Promega; Madison, WI) and 200 U of Superscript II reverse transcriptase (Life Technologies/Invitrogen; Carlsbad, CA) were added to the mixture. The reaction proceeded at 42°C for 1 h, after which an additional 200 U of Superscript II reverse transcriptase was added and the reaction mixture was incubated at 42°C for an additional hour. To stop the reaction, EDTA (pH 8.0) and sodium hydroxide were added to final concentrations of 5 mM and 0.4 N, respectively, and the mixture was incubated at 65°C for 20 min. Finally, acetic acid was added to achieve a final concentration of 0.37 M.

The labeled cDNA probes were purified using QIAquick columns (QIAGEN, Valencia, CA) and following the manufacturer's instructions. The cDNA probes were then fluorescently labeled. One set of cDNA probes was labeled using Cy5 for those representing RNA from drug-treated cells and Cy3 for those representing RNA from control cells. The second set was labeled using Cy3 for those representing RNA from drug-treated cells and Cy5 for those representing RNA from control cells. Five microliters each of the Cy3- and Cy5-labeled probes was mixed with 50 µg of heat-denatured salmon sperm DNA, incubated at 95°C for 2 min, and snap-cooled on ice. The mixture was added to 40 µl of hybridization buffer (DIG easy hyb; Roche, Basel, Switzerland) and applied to the array slides under glass coverslips. Hybridization was performed at 37°C overnight in a humidified chamber (Corning Life Sciences; Acton, MA). To wash the slides, the coverslip was removed and the slide was incubated at room temperature in 0.2x SSC (1x SSC is 0.15 M NaCl plus 0.015 M sodium citrate)-0.1% sodium dodecyl sulfate for 5 min with agitation, rinsed at room temperature with 0.2x SSC for 5 min with agitation, and spin dried at 500 rpm for 5 min. Slides were scanned using a ChipReader microarray scanner (Virtek Vision Intl.).

Data analysis. GenePix 1.0 software (Axon Instruments, Inc.) was used for image analysis and data visualization. The local background values were calculated from the area surrounding each spot and subtracted from the total spot signal values. These adjusted values were used to determine differential gene expression (Cy3/Cy5 ratio) for each spot. A normalization factor was applied to account for systematic differences in the probe labels by using the differential gene expression ratio to balance the Cy5 signals. In the present study, only spots with a mean balanced differential expression ratio greater than or equal to 1.5 or less than or equal to 0.67 (–1.5) for both spots representing a given cDNA on the array in two independent experiments were considered to be differentially expressed.

DNA sequences were annotated on the basis of results of BLASTn searches using GenBank (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi), the Stanford sequencing database (Stanford University, Palo Alto, CA; http://www-sequence.stanford.edu/group/candida), and the CandidaDB database (http://www.pasteur.fr/Galar_Fungail/CandidaDB/).

Quantitative real-time RT-PCR. An aliquot of the RNA preparations from untreated and treated samples, used in the microarray experiments, was saved for quantitative real-time reverse transcription (RT)-PCR follow-up studies. First-strand cDNAs were synthesized from 2 µg of total RNA in a 21-µl reaction volume using the SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA) in accordance with the manufacturer's instructions. Quantitative real-time PCRs were performed in triplicate using the 7000 Sequence Detection System (Applied Biosystems, Foster City, CA). Independent PCRs were performed using the same cDNA for both the gene of interest and the 18S rRNA, using the SYBR Green PCR Master Mix (Applied Biosystems). Gene-specific primers were designed for the gene of interest and the 18S rRNA using Primer Express software (Applied Biosystems) and the Oligo Analysis & Plotting Tool (QIAGEN, Valencia, CA) and are shown in Table 1. The PCR conditions consisted of AmpliTaq Gold activation at 95°C for 10 min, followed by 40 cycles of denaturation at 95°C for 15 s and annealing/extension at 60°C for 1 min. A dissociation curve was generated at the end of each PCR cycle to verify that a single product was amplified using software provided with the 7000 Sequence Detection System. The change in fluorescence of SYBR Green I dye in every cycle was monitored by the system software, and the threshold cycle (C_T) above the background for each reaction was calculated. The C_T value of 18S rRNA was subtracted from that of the gene of interest to obtain a C_T value. The C_T value of an arbitrary calibrator (e.g., untreated sample) was subtracted from the C_T value of each sample to obtain a C_T value. The gene expression level relative to the calibrator was expressed as 2^–CT.

View this table: [in this window] [in a new window]

TABLE 1. Gene-specific primers used for real-time RT-PCR assays

Top Abstract Introduction Materials and Methods Results Discussion References

 
Gene expression responses to antifungal drug exposure. A total of 82 genes were found to be differentially expressed upon exposure to KTZ. Of these, 60 showed an increase in expression and 22 showed a decrease in expression. The distribution of KTZ-responsive genes and their biological roles are shown in Fig. 1. The category of genes with the largest number of responses was that of unknown function (33%), followed by carbohydrate metabolism (16%); small-molecule transport (16%); lipid, fatty acid, and sterol metabolism (15%); and cell stress (10%).

View larger version (37K): [in this window] [in a new window]

FIG. 1. Distribution of KTZ-responsive genes. Genes were annotated and assigned to functional categories. The average expression ratio from two independent experiments is shown. Genes shaded in gray are indicative of major responses associated with the drug's mechanism of action. Genes in boldface have been previously associated with azole resistance.

A total of 256 genes were found to be differentially expressed upon exposure to AMB. Of these, 87 showed an increase in expression and 169 showed a decrease in expression. The distribution of AMB-responsive genes and their biological roles are shown in Fig. 2. The category of genes with the largest number of responses was that of unknown function (20%), followed by protein synthesis (14%), small-molecule transport (8%), cell stress (6%), and lipid, fatty acid, and sterol metabolism (6%).

View larger version (44K): [in this window] [in a new window]

FIG. 2. Distribution of AMB-responsive genes. Gene annotations and expression values are as in Fig. 1. Genes shaded in gray are indicative of major responses associated with the drug's mechanism of action.

A total of 480 genes were found to be differentially expressed upon exposure to CPF. Of these, 81 showed an increase in expression and 399 showed a decrease in expression. The distribution of CPF-responsive genes and their biological roles are shown in Fig. 3. The category of genes with the largest number of responses was that of unknown function (26%). Of particular interest, however, were the responses by genes in the categories of small-molecule transport (8%); cell stress (6%); lipid, fatty acid, and sterol metabolism (3%); and cell wall maintenance (3%).

View larger version (49K): [in this window] [in a new window]

FIG. 3. Distribution of CPF-responsive genes. Gene annotations and expression values are as in Fig. 1. Genes shaded in gray are indicative of major responses associated with the drug's mechanism of action.

A total of 439 genes were found to be differentially expressed upon exposure to 5-FC. Of these, 383 showed an increase in expression and 56 showed a decrease in expression. The distribution of 5-FC-responsive genes and their biological roles are shown in Fig. 4. The category of genes with the largest number of responses was that of unknown function (26%), followed by protein synthesis (18%). Of particular interest were the responses by genes in the categories of RNA processing/modification/splicing (3%), DNA repair/synthesis (2%), and nucleotide metabolism (2%).

View larger version (46K): [in this window] [in a new window]

FIG. 4. Distribution of 5-FC-responsive genes. Gene annotations and expression values are as in Fig. 1. Genes shaded in gray are indicative of major responses associated with the drug's mechanism of action.

A complete list of all genes found to be differentially expressed in response to at least one drug can be found in the supplemental material (Table S2). There were no genes that exhibited similar changes in expression for all four drugs. Two genes responded similarly to AMB, KTZ, and CPF. These were DDR48 (up-regulated) and FET33 (down-regulated). A complete list of genes similarly differentially expressed in response to at least two of the four drugs can be viewed in the supplemental material (Table S3).

Validation of microarray data by real-time RT-PCR. To validate the differential expression of genes identified by microarray analysis, we performed real-time RT-PCR for nine genes of interest. These were selected based on their role in the specific mechanism of action of these drugs (e.g., ERG11 for KTZ, CDC21 for 5-FC, and GSL22 for CPF) but also to verify responses independent of mechanism of action (e.g., CDR1 for KTZ, SKN1.3 for CPF, YNK1 for 5-FC, and CTA1 and YHB1 for AMB). In general, there was a good correlation between real-time RT-PCR and microarray data (Fig. 5A). However, for some genes (SKN1.3, YNK1, and DDR48), we observed greater n-fold changes in expression by real-time RT-PCR than with microarray analysis. This may be reflective of the greater dynamic range of real-time RT-PCR analysis. Furthermore, in situations where poor or no hybridization signals are generated for one of the samples tested, n-fold induction values can be under- or overestimated.

View larger version (11K): [in this window] [in a new window]

FIG. 5. Quantitative real-time RT-PCR analysis of genes identified as differentially expressed by microarray experiments. Eight genes identified as differentially expressed by microarray analysis were examined by quantitative real-time RT-PCR with gene-specific primers. Data are shown as mean ± S.D. (A) Gene expression changes in 8 genes (2 per drug) detected by real-time RT-PCR. (B) Validation of drug-specific gene expression responses.

In order to confirm the drug specificity of some of these responses, we examined changes in expression of CDR1 in response to 5-FC, CPF, and AMB; SKN1.3 in response to KTZ, 5-FC, and AMB; and YNK1 in response to KTZ, CPF, and AMB. We also chose to examine expression of DDR48 as it responded similarly to three of the four antifungal agents used in this study. Results of these assays indicate that the changes in expression of these genes are drug specific (Fig. 5B).

Top Abstract Introduction Materials and Methods Results Discussion References

 
Gene expression responses to KTZ. Genes responsive to KTZ included the ergosterol biosynthesis genes NCP1, MCR1, CYB5, ERG2, ERG3, POT14 (ERG10), ERG25, and ERG251 and the gene encoding the target of the azoles, ERG11. This is somewhat similar to that previously observed in S. cerevisiae. With the exception of POT14 (ERG10), most of the products of these genes function downstream of lanosterol demethylase, suggesting that their induction is in response to ergosterol depletion. Alternatively, up-regulation of ERG25 may serve to facilitate the conversion of lanosterol to alternative membrane sterols such as obtusifoliol and 14-methyl fecosterol.

In addition to the ergosterol biosynthesis genes ERG2, ERG3, POT14 (ERG10), ERG11, and ERG25, KTZ induced the expression of the multidrug resistance genes CDR1 and CDR2, as well as other genes found to be differentially expressed in azole-resistant isolates such as RTA3, IFD4, and DDR48 (15, 27, 28). RTA3 is a gene of unknown function that is coregulated with CDR1, CDR2, and ERG2 in azole-resistant isolates (28). It is homologous to RTA1 in S. cerevisiae, which has been implicated in resistance to the ergosterol biosynthesis inhibitor 7-aminocholoesterol (34). IFD4 (also known as CSH1) is a member of a family of homologs of YPL088w in S. cerevisiae (31). While its function is unknown, it has been shown to play a key role in cell surface hydrophobicity in C. albicans. It should be noted that YPL088w was among the KTZ-responsive genes previously identified in S. cerevisiae (1). DDR48 is homologous to a gene in S. cerevisiae that encodes a DNA damage-inducible protein whose expression has been shown to be responsive to cellular stresses (22, 23).

The observation that KTZ induced expression of UPC2 and associated genes in S. cerevisiae was a key finding of our previous study (1). In S. cerevisiae, UPC2 is a transcriptional regulator of sterol uptake under anaerobic conditions (38). Absence of the response of a similar pathway in C. albicans is not surprising as C. albicans does not appear to take up exogenous sterols (36). Given the fact that C. albicans resides primarily in the gastrointestinal tracts of humans, it is possible that this organism has developed alternative strategies for dealing with anaerobic environments, as has recently been suggested by others (13).

Only one other study has examined the effects of azole treatment on the gene expression profile of C. albicans (9). Several genes were similarly differentially expressed between that study and the present study, including ERG2, ERG3, POT14 (ERG10), ERG11, ERG25, NCP1, CYB5, SAH1, DDR48, CWH8, and CTR1. Differences in the results of these studies can be explained by the differences between the drugs used and the experimental designs employed. In the previous study, C. albicans was exposed to itraconazole for 24 h (9). In the present study, we exposed C. albicans to a different azole (KTZ) for a shorter time and at a lower effective dose. We would expect changes in gene expression observed in our study to specifically reflect the acute response to azole-induced cell stress.

Gene expression responses to AMB. Responsive genes in the small-molecule category included up-regulation of those involved in calcium (IPF11550, IPF11560), potassium (IPF9136), sodium (ENA21), zinc (ZRT2), and sulfate (SUL1) transport and down-regulation of those involved in phosphate (PHO88), hydrogen (VMA2), citrate (CTP1), copper (CTR1), and iron (SIT1, FRE30, FRE31, FTR1, FTR2, FTH1, CFL1, CFL2, FET33) transport. IPF11550 and IPF11560 are homologs of the S. cerevisiae gene PMR1, which encodes a P-type calcium ATPase in the Golgi apparatus involved in regulating cytosolic calcium levels (2, 29). ENA21 is homologous to the S. cerevisiae gene ENA5, which appears to encode a sodium transporter (39), whereas IPF9136 appears to encode a potassium transporter homologous to Hak1p in the yeast Schwanniomyces occidentalis (4). Up-regulation of these genes suggests active removal of calcium and sodium from the cytoplasm and is consistent with disruption of the plasma membrane by AMB. Up-regulation of IPF9136, as well as the S. cerevisiae SAT4/HAL4 homolog IPF11548, is suggestive of the active uptake of extracellular potassium. This is consistent with the ability of AMB to induce the release of potassium in C. albicans, which is thought to be the primary mechanism of action of this drug (7). Of interest was the down-regulation of genes involved in iron uptake, suggesting that AMB exposure produces a state of iron overload.

In addition to its deleterious effects on the plasma membrane of C. albicans, AMB is also believed to cause oxidative damage to fungal cells (32, 33). It is not surprising that a number of oxidative stress response genes were up-regulated in response to this agent. These included YHB1, AOX1, AOX2, CTA1, SOD2, and GSH1. YHB1 encodes flavohemoglobin, which in S. cerevisiae appears to protect cells against the damage caused by Cu²⁺ and dithiothreitol while sensitizing them to H₂O₂ (8). Recently, YHB1 in C. albicans was shown to be inducible by nitric oxide and to be required for protection against nitric oxide toxicity (37). AOX1 and presumably AOX2 encode alternative oxidases which have been associated with the oxidative stress response in some species (16). SOD2 encodes a mitochondrial manganese superoxide dismutase. Disruption of SOD2 has been shown to confer hypersensitivity to various stresses, such as redox-cycling agents, heating, ethanol, high concentrations of sodium or potassium, and 99.9% O₂ (17). In S. cerevisiae, GSH1 encodes -glutamylcysteine synthetase, which catalyzes a key step in glutathione biosynthesis (12). CTA1 encodes peroxisomal catalase A, an antioxidant enzyme (40). Interestingly, clinical isolates lacking membrane ergosterol and exhibiting reduced susceptibility to AMB have been shown to have greater levels of catalase activity (32). Collectively, the up-regulation of oxidative stress response genes upon exposure to AMB is consistent with the proposed oxidative damage caused by this antifungal agent.

Since AMB targets the membrane sterol ergosterol, it was significant to observe changes in the expression of genes involved in lipid, fatty acid, and sterol metabolism. These included the up-regulation of MVD1 (ERG19) and the down-regulation of the ergosterol biosynthesis genes ERG26, ERG16 (ERG11), ERG9, ERG25, ERG13, ERG251, and ERG3, the fatty acid biosynthesis genes FEN12, FAS1, FAS2, and ACB1, and the phospholipid biosynthesis genes CHO2 and OPI3. Down-regulation of certain ergosterol biosynthesis genes in response to AMB may be reflective of an attempt by the organism to use alternate sterols or sterol intermediates in the cell membrane. We recently examined the gene expression profile of an AMB- and fluconazole-resistant C. albicans laboratory-derived isolate (5). This isolate exhibited changes in sterol composition consistent with a defective lanosterol demethylase. Several genes are similarly differentially expressed between AMB exposure in the present study and AMB resistance in the previous study. These include up-regulation of DDR48, CTA1, IPF19801, and IPF3964 and down-regulation of TYE7, NHP6A, UBI4, HTB1, HFF22, HHT21, HHF21, GPX2, IPF11785, and IPF11625. It is tempting to speculate that these changes in gene expression are coordinately regulated in response to reduced ergosterol biosynthesis.

Gene expression responses to CPF. The cell wall, which is primarily responsible for maintaining cell shape and osmotic stability, is composed of mannoproteins and chitin, as well as ß-1,3-glucan and ß-1,6-glucan. ß-1,3-Glucan is the central component to which all other components are cross-linked (19, 20). CPF is thought to inhibit the ß-1,3-glucan synthase activity of the FKS1 and FKS2 gene products in S. cerevisiae (10). The C. albicans homologs of these genes are GSL21 and GSL23, respectively. In the present study, CPF induced the expression of several genes involved in cell wall maintenance, including the gene encoding a third ß-1,3-glucan synthase subunit, GSL22, which is homologous to S. cerevisiae FKS3.

Other cell wall maintenance genes that were up-regulated included CRH11, PHR1, IPF8537, ECM331, SKN1.3, and KRE1, and ECM15, FEN12, SMI1, PPH21, SCW1, SUN41, IPF19968, SCW11, ECM21, ASC4, and ECM33 were down-regulated. Several homologs of these genes have recently been implicated in ß-1,3-glucan assembly in S. cerevisiae, including the PHR1 homolog GAS1, KRE1, SWI1, the FEN12 homolog FEN1, and ECM21 (21). Disruption of FKS1 in S. cerevisiae resulted in up-regulation of the CRH11 homolog CRH1 and the ECM331 homolog PST1 (35). Furthermore, these genes, as well as GSC2 (FKS2), were up-regulated in response to CPF in S. cerevisiae (1). Indeed, changes in the expression of these genes are suggestive of alterations in the cell wall that are consistent with the mechanism of action of this antifungal agent.

Gene expression responses to 5-FC. 5-FC enters the fungal cell via cytosine permease (25), where it is converted to 5-fluoroucil (5FU) by cytosine deaminase. 5FU is then converted to 5-fluorouridine monophosphate by the FUR1 gene product uracil phosphoribosyltransferase. 5-Fluorouridine monophosphate is subsequently converted to 5-fluorouridine triphosphate, which ultimately interferes with protein synthesis through incorporation into RNA in lieu of UTP (18). 5FU also undergoes conversion to 5-fluorodeoxyuridine monophosphate, which inhibits thymidylate synthetase, encoded by CDC21, by disrupting DNA synthesis (11, 18, 25, 26). It is therefore not surprising that we observed increased expression of a large number of genes involved in protein synthesis, as well as the purine and pyrimidine biosynthesis genes IMH3, YNK1, IPF6105, URA2, URA5, URA7, ADE4, ADE5, ADE7, PRS3, FUR1 and CDC21. The changes in gene expression we observed in response to 5-FC exposure are consistent with a need for the cell to increase RNA, DNA, and protein production, as well as uracil phosphoribosyltransferase and thymidylate synthetase production.

Responses common to multiple drugs. As noted above, DDR48 is homologous to a gene in S. cerevisiae that encodes a DNA damage-inducible protein whose expression has been shown to be responsive to cellular stresses (22, 23). FET33 is homologous to FET3 in S. cerevisiae, which encodes a ferroxidase required for high-affinity iron uptake (3). There were many genes that responded similarly to at least two of the four drugs (Table S3 in the supplemental material). Most striking was the set of genes that were similarly regulated by both AMB and CPF. This included the down-regulation of genes involved in iron regulation (CFL1, CFL2, FTR1, FTR2, CTR1, and FTH1), chromatin/chromosome structure (HHF21, HHT21, HHF22, HTA1, HHT3, HTB1, and NHP6A), and lipid, fatty acid, and sterol metabolism (ERG25, ERG251, SAH1, ERG3, OPI3, ERG13, FAS1, and ERG9). As both AMB and CPF are fungicidal agents, it is tempting to speculate that these commonly differentially expressed genes reflect a response to compromised cellular integrity.

In conclusion, the studies reported here have revealed antifungal drug-specific changes in gene expression consistent with known mechanisms of action and in some cases suggest novel effects of these drugs. We also identify responses shared across multiple classes of antifungal agent. C. albicans gene expression profiling is a useful tool for understanding mechanisms of action and potentially mechanisms of resistance for antifungal agents with activity against this pathogenic fungus.

 
This work was supported in part by a grant from the United States Public Health Service, NIAID R01 AI49770 from the National Institutes of Health through the University of Mississippi.

The contents of this report are solely the responsibility of the authors and do not necessarily represent the official view of the University of Mississippi.

 
* Corresponding author. Mailing address: Le Bonheur Children's Medical Center, Room 304 West Patient Tower, Children's Foundation Research Center, 50 North Dunlap Street, Memphis, TN 38163. Phone: (901) 448-3719. Fax: (901) 448-6064. E-mail: drogers{at}utmem.edu.

Supplemental material for this article may be found at http://aac.asm.org/.

Top Abstract Introduction Materials and Methods Results Discussion References

 

1
1. Agarwal, A. K., P. D. Rogers, S. R. Baerson, M. R. Jacob, K. S. Barker, J. D. Cleary, L. A. Walker, D. G. Nagle, and A. M. Clark. 2003. Genome-wide expression profiling of the response to polyene, pyrimidine, azole, and echinocandin antifungal agents in Saccharomyces cerevisiae. J. Biol. Chem. 278:34998-35015.[Abstract/Free Full Text]
2
2. Antebi, A., and G. R. Fink. 1992. The yeast Ca²⁺-ATPase homologue, PMR1, is required for normal Golgi function and localizes in a novel Golgi-like distribution. Mol. Biol. Cell 3:633-654.[Abstract]
3
3. Askwith, C., D. Eide, A. Van Ho, P. S. Bernard, L. Li, S. Davis-Kaplan, D. M. Sipe, and J. Kaplan. 1994. The FET3 gene of S. cerevisiae encodes a multicopper oxidase required for ferrous iron uptake. Cell 76:403-410.[CrossRef][Medline]
4
4. Banuelos, M. A., R. Madrid, and A. Rodriguez-Navarro. 2000. Individual functions of the HAK and TRK potassium transporters of Schwanniomyces occidentalis. Mol. Microbiol. 37:671-679.[CrossRef][Medline]
5
5. Barker, K. S., S. Crisp, N. Wiederhold, R. E. Lewis, B. Bareither, J. Eckstein, R. Barbuch, M. Bard, and P. D. Rogers. 2004. Genome-wide expression profiling reveals genes associated with amphotericin B and fluconazole resistance in experimentally induced antifungal resistant isolates of Candida albicans. J. Antimicrob. Chemother. 54(2):376-385.[Abstract/Free Full Text]
6
6. Beck-Sague, C. M., and W. R. Jarvis. 1993. Secular trends in the epidemiology of nosocomial fungal infections in the United States, 1980-1990. National Nosocomial Infections Surveillance. Syst. J. Infect. Dis. 167:1247-1251.
7
7. Brajtburg, J., W. G. Powderly, G. S. Kobayashi, and G. Medoff. 1990. Amphotericin B: current understanding of mechanisms of action. Antimicrob. Agents Chemother. 34:183-188.[Free Full Text]
8
8. Buisson, N., and R. Labbe-Bois. 1998. Flavohemoglobin expression and function in Saccharomyces cerevisiae. No relationship with respiration and complex response to oxidative stress. J. Biol. Chem. 273:9527-9533.[Abstract/Free Full Text]
9
9. De Backer, M. D., T. Ilyina, X. J. Ma, S. Vandoninck, W. H. Luyten, and H. Vanden Bossche. 2001. Genomic profiling of the response of Candida albicans to itraconazole treatment using a DNA microarray. Antimicrob. Agents Chemother. 45:1660-1670.[Abstract/Free Full Text]
10
10. Deresinski, S. C., and D. A. Stevens. 2003. Caspofungin. Clin. Infect. Dis. 36:1445-1457.[CrossRef][Medline]
11
11. Diasio, R. B., J. E. Bennett, and C. E. Myers. 1978. Mode of action of 5-fluorocytosine. Biochem. Pharmacol. 27:703-707.[CrossRef][Medline]
12
12. Dormer, U. H., J. Westwater, D. W. Stephen, and D. J. Jamieson. 2002. Oxidant regulation of the Saccharomyces cerevisiae GSH1 gene. Biochim. Biophys Acta 1576:23-29.[Medline]
13
13. Dumitru, R., J. M. Hornby, and K. W. Nickerson. 2004. Defined anaerobic growth medium for studying Candida albicans basic biology and resistance to eight antifungal drugs. Antimicrob. Agents Chemother. 48:2350-2354.[Abstract/Free Full Text]
14
14. Edmond, M. B., S. E. Wallace, D. K. McClish, M. A. Pfaller, R. N. Jones, and R. P. Wenzel. 1999. Nosocomial bloodstream infections in United States hospitals: a three-year analysis. Clin. Infect. Dis. 29:239-244.[Medline]
15
15. Hooshdaran, M. Z., K. S. Barker, G. M. Hilliard, H. Kusch, J. Morschhauser, and P. D. Rogers. 2004. Proteomic analysis of azole resistance in Candida albicans clinical isolates. Antimicrob. Agents Chemother. 48:2733-2735.[Abstract/Free Full Text]
16
16. Huh, W. K., and S. O. Kang. 1999. Molecular cloning and functional expression of alternative oxidase from Candida albicans. J. Bacteriol. 181:4098-4102.[Abstract/Free Full Text]
17
17. Hwang, C. S., Y. U. Baek, H. S. Yim, and S. O. Kang. 2003. Protective roles of mitochondrial manganese-containing superoxide dismutase against various stresses in Candida albicans. Yeast 20:929-941.[CrossRef][Medline]
18
18. Jund, R., and F. Lacroute. 1970. Genetic and physiological aspects of resistance to 5-fluoropyrimidines in Saccharomyces cerevisiae. J. Bacteriol. 102:607-615.[Abstract/Free Full Text]
19
19. Klis, F. M., P. de Groot, and K. Hellingwerf. 2001. Molecular organization of the cell wall of Candida albicans. Med. Mycol. 39(Suppl. 1):1-8.
20
20. Klis, F. M., P. Mol, K. Hellingwerf, and S. Brul. 2002. Dynamics of cell wall structure in Saccharomyces cerevisiae. FEMS Microbiol. Rev. 26:239-256.[CrossRef][Medline]
21
21. Lesage, G., A. M. Sdicu, P. Menard, J. Shapiro, S. Hussein, and H. Bussey. 2004. Analysis of beta-1,3-glucan assembly in Saccharomyces cerevisiae using a synthetic interaction network and altered sensitivity to caspofungin. Genetics 167:35-49.[Abstract/Free Full Text]
22
22. Miralles, V. J., and R. Serrano. 1995. A genomic locus in Saccharomyces cerevisiae with four genes up-regulated by osmotic stress. Mol. Microbiol. 17:653-662.[CrossRef][Medline]
23
23. Momose, Y., and H. Iwahashi. 2001. Bioassay of cadmium using a DNA microarray: genome-wide expression patterns of Saccharomyces cerevisiae response to cadmium. Environ. Toxicol. Chem. 20:2353-2360.[CrossRef][Medline]
24
24. Pfaller, M. A., R. N. Jones, S. A. Messer, M. B. Edmond, and R. P. Wenzel. 1998. National surveillance of nosocomial blood stream infection due to Candida albicans: frequency of occurrence and antifungal susceptibility in the SCOPE Program. Diagn. Microbiol. Infect. Dis. 31:327-332.[CrossRef][Medline]
25
25. Polak, A., and M. Grenson. 1973. Evidence for a common transport system for cytosine, adenine and hypoxanthine in Saccharomyces cerevisiae and Candida albicans. Eur. J. Biochem. 32:276-282.[Medline]
26
26. Polak, A., and H. J. Scholer. 1975. Mode of action of 5-fluorocytosine and mechanisms of resistance. Chemotherapy 21:113-130.[Medline]
27
27. Rogers, P. D., and K. S. Barker. 2002. Evaluation of differential gene expression in fluconazole-susceptible and -resistant isolates of Candida albicans by cDNA microarray analysis. Antimicrob. Agents Chemother. 46:3412-3417.[Abstract/Free Full Text]
28
28. Rogers, P. D., and K. S. Barker. 2003. Genome-wide expression profile analysis reveals coordinately regulated genes associated with stepwise acquisition of azole resistance in Candida albicans clinical isolates. Antimicrob. Agents Chemother. 47:1220-1227.[Abstract/Free Full Text]
29
29. Rudolph, H. K., A. Antebi, G. R. Fink, C. M. Buckley, T. E. Dorman, J. LeVitre, L. S. Davidow, J. I. Mao, and D. T. Moir. 1989. The yeast secretory pathway is perturbed by mutations in PMR1, a member of a Ca²⁺ ATPase family. Cell 58:133-145.[CrossRef][Medline]
30
30. Schmitt, M. E., T. A. Brown, and B. L. Trumpower. 1990. A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae. Nucleic Acids Res. 18:3091-3092.[Free Full Text]
31
31. Singleton, D. R., and K. C. Hazen. 2004. Differential surface localization and temperature-dependent expression of the Candida albicans CSH1 protein. Microbiology 150:285-292.[Abstract/Free Full Text]
32
32. Sokol-Anderson, M., J. E. Sligh, Jr., S. Elberg, J. Brajtburg, G. S. Kobayashi, and G. Medoff. 1988. Role of cell defense against oxidative damage in the resistance of Candida albicans to the killing effect of amphotericin B. Antimicrob. Agents Chemother. 32:702-705.[Abstract/Free Full Text]
33
33. Sokol-Anderson, M. L., J. Brajtburg, and G. Medoff. 1986. Amphotericin B-induced oxidative damage and killing of C. albicans. J. Infect. Dis. 154:76-83.[Medline]
34
34. Soustre, I., Y. Letourneux, and F. Karst. 1996. Characterization of the Saccharomyces cerevisiae RTA1 gene involved in 7-aminocholesterol resistance. Curr. Genet. 30:121-125.[CrossRef][Medline]
35
35. Terashima, H., N. Yabuki, M. Arisawa, K. Hamada, and K. Kitada. 2000. Up-regulation of genes encoding glycosylphosphatidylinositol (GPI)-attached proteins in response to cell wall damage caused by disruption of FKS1 in Saccharomyces cerevisiae. Mol. Gen. Genet. 264:64-74.[CrossRef][Medline]
36
36. Tsai, H. F., M. Bard, K. Izumikawa, A. A. Krol, A. M. Sturm, N. T. Culbertson, C. A. Pierson, and J. E. Bennett. 2004. Candida glabrata erg1 mutant with increased sensitivity to azoles and to low oxygen tension. Antimicrob. Agents Chemother. 48:2483-2489.[Abstract/Free Full Text]
37
37. Ullmann, B. D., H. Myers, W. Chiranand, A. L. Lazzell, Q. Zhao, L. A. Vega, J. L. Lopez-Ribot, P. R. Gardner, and M. C. Gustin. 2004. Inducible defense mechanism against nitric oxide in Candida albicans. Eukaryot. Cell 3:715-723.[Abstract/Free Full Text]
38
38. Vik, A., and J. Rine. 2001. Upc2p and Ecm22p, dual regulators of sterol biosynthesis in Saccharomyces cerevisiae. Mol. Cell. Biol. 21:6395-6405.[Abstract/Free Full Text]
39
39. Wieland, J., A. M. Nitsche, J. Strayle, H. Steiner, and H. K. Rudolph. 1995. The PMR2 gene cluster encodes functionally distinct isoforms of a putative Na⁺ pump in the yeast plasma membrane. EMBO J. 14:3870-3882.[Medline]
40
40. Wysong, D. R., L. Christin, A. M. Sugar, P. W. Robbins, and R. D. Diamond. 1998. Cloning and sequencing of a Candida albicans catalase gene and effects of disruption of this gene. Infect. Immun. 66:1953-1961.[Abstract/Free Full Text]

---

Antimicrobial Agents and Chemotherapy, June 2005, p. 2226-2236, Vol. 49, No. 6
0066-4804/05/$08.00+0     doi:10.1128/AAC.49.6.2226-2236.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Miller, M. B., Tang, Y.-W. (2009). Basic Concepts of Microarrays and Potential Applications in Clinical Microbiology. Clin. Microbiol. Rev. 22: 611-633 [Abstract] [Full Text]  
• Sellam, A., Tebbji, F., Nantel, A. (2009). Role of Ndt80p in Sterol Metabolism Regulation and Azole Resistance in Candida albicans. Eukaryot Cell 8: 1174-1183 [Abstract] [Full Text]  
• Gautam, P., Shankar, J., Madan, T., Sirdeshmukh, R., Sundaram, C. S., Gade, W. N., Basir, S. F., Sarma, P. U. (2008). Proteomic and Transcriptomic Analysis of Aspergillus fumigatus on Exposure to Amphotericin B. Antimicrob. Agents Chemother. 52: 4220-4227 [Abstract] [Full Text]  
• Song, Y., Cheon, S. A., Lee, K. E., Lee, S.-Y., Lee, B.-K., Oh, D.-B., Kang, H. A., Kim, J.-Y. (2008). Role of the RAM Network in Cell Polarity and Hyphal Morphogenesis in Candida albicans. Mol. Biol. Cell 19: 5456-5477 [Abstract] [Full Text]  
• Chaffin, W. L. (2008). Candida albicans Cell Wall Proteins. Microbiol. Mol. Biol. Rev. 72: 495-544 [Abstract] [Full Text]  
• Cui, L., Miao, J., Furuya, T., Fan, Q., Li, X., Rathod, P. K., Su, X.-z., Cui, L. (2008). Histone Acetyltransferase Inhibitor Anacardic Acid Causes Changes in Global Gene Expression during In Vitro Plasmodium falciparum Development. Eukaryot Cell 7: 1200-1210 [Abstract] [Full Text]  
• Dunkel, N., Liu, T. T., Barker, K. S., Homayouni, R., Morschhauser, J., Rogers, P. D. (2008). A Gain-of-Function Mutation in the Transcription Factor Upc2p Causes Upregulation of Ergosterol Biosynthesis Genes and Increased Fluconazole Resistance in a Clinical Candida albicans Isolate. Eukaryot Cell 7: 1180-1190 [Abstract] [Full Text]  
• Rauceo, J. M., Blankenship, J. R., Fanning, S., Hamaker, J. J., Deneault, J.-S., Smith, F. J., Nantel, A., Mitchell, A. P. (2008). Regulation of the Candida albicans Cell Wall Damage Response by Transcription Factor Sko1 and PAS Kinase Psk1. Mol. Biol. Cell 19: 2741-2751 [Abstract] [Full Text]  
• Hodgetts, S., Nooney, L., Al-Akeel, R., Curry, A., Awad, S., Matthews, R., Burnie, J. (2008). Efungumab and caspofungin: pre-clinical data supporting synergy. J Antimicrob Chemother 61: 1132-1139 [Abstract] [Full Text]  
• Cowen, L. E., Steinbach, W. J. (2008). Stress, Drugs, and Evolution: the Role of Cellular Signaling in Fungal Drug Resistance. Eukaryot Cell 7: 747-764 [Full Text]  
• Znaidi, S., Weber, S., Zin Al-Abdin, O., Bomme, P., Saidane, S., Drouin, S., Lemieux, S., De Deken, X., Robert, F., Raymond, M. (2008). Genomewide Location Analysis of Candida albicans Upc2p, a Regulator of Sterol Metabolism and Azole Drug Resistance. Eukaryot Cell 7: 836-847 [Abstract] [Full Text]  
• Sosinska, G. J., de Groot, P. W. J., Teixeira de Mattos, M. J., Dekker, H. L., de Koster, C. G., Hellingwerf, K. J., Klis, F. M. (2008). Hypoxic conditions and iron restriction affect the cell-wall proteome of Candida albicans grown under vagina-simulative conditions. Microbiology 154: 510-520 [Abstract] [Full Text]  
• Barelle, C. J., Duncan, V. M. S., Brown, A. J. P., Gow, N. A. R., Odds, F. C. (2008). Azole antifungals induce up-regulation of SAP4, SAP5 and SAP6 secreted proteinase genes in filamentous Candida albicans cells in vitro and in vivo. J Antimicrob Chemother 61: 315-322 [Abstract] [Full Text]  
• Banerjee, D., Lelandais, G., Shukla, S., Mukhopadhyay, G., Jacq, C., Devaux, F., Prasad, R. (2008). Responses of Pathogenic and Nonpathogenic Yeast Species to Steroids Reveal the Functioning and Evolution of Multidrug Resistance Transcriptional Networks. Eukaryot Cell 7: 68-77 [Abstract] [Full Text]  
• Meyer, V., Damveld, R. A., Arentshorst, M., Stahl, U., van den Hondel, C. A. M. J. J., Ram, A. F. J. (2007). Survival in the Presence of Antifungals: GENOME-WIDE EXPRESSION PROFILING OF ASPERGILLUS NIGER IN RESPONSE TO SUBLETHAL CONCENTRATIONS OF CASPOFUNGIN AND FENPROPIMORPH. J. Biol. Chem. 282: 32935-32948 [Abstract] [Full Text]  
• Morton, C. O., Hayes, A., Wilson, M., Rash, B. M., Oliver, S. G., Coote, P. (2007). Global Phenotype Screening and Transcript Analysis Outlines the Inhibitory Mode(s) of Action of Two Amphibian-Derived, {alpha}-Helical, Cationic Peptides on Saccharomyces cerevisiae. Antimicrob. Agents Chemother. 51: 3948-3959 [Abstract] [Full Text]  
• Norice, C. T., Smith, F. J. Jr., Solis, N., Filler, S. G., Mitchell, A. P. (2007). Requirement for Candida albicans Sun41 in Biofilm Formation and Virulence. Eukaryot Cell 6: 2046-2055 [Abstract] [Full Text]  
• Cannon, R. D., Lamping, E., Holmes, A. R., Niimi, K., Tanabe, K., Niimi, M., Monk, B. C. (2007). Candida albicans drug resistance another way to cope with stress. Microbiology 153: 3211-3217 [Abstract] [Full Text]  
• Vylkova, S., Jang, W. S., Li, W., Nayyar, N., Edgerton, M. (2007). Histatin 5 Initiates Osmotic Stress Response in Candida albicans via Activation of the Hog1 Mitogen-Activated Protein Kinase Pathway. Eukaryot Cell 6: 1876-1888 [Abstract] [Full Text]  
• Jezewski, S., von der Heide, M., Poltermann, S., Hartl, A., Kunkel, W., Zipfel, P. F., Eck, R. (2007). Role of the Vps34p-interacting protein Ade5,7p in hyphal growth and virulence of Candida albicans. Microbiology 153: 2351-2362 [Abstract] [Full Text]  
• Yu, L., Zhang, W., Wang, L., Yang, J., Liu, T., Peng, J., Leng, W., Chen, L., Li, R., Jin, Q. (2007). Transcriptional Profiles of the Response to Ketoconazole and Amphotericin B in Trichophyton rubrum. Antimicrob. Agents Chemother. 51: 144-153 [Abstract] [Full Text]  
• Brown, V., Sexton, J. A., Johnston, M. (2006). A Glucose Sensor in Candida albicans.. Eukaryot Cell 5: 1726-1737 [Abstract] [Full Text]  
• Lepak, A., Nett, J., Lincoln, L., Marchillo, K., Andes, D. (2006). Time Course of Microbiologic Outcome and Gene Expression in Candida albicans during and following In Vitro and In Vivo Exposure to Fluconazole.. Antimicrob. Agents Chemother. 50: 1311-1319 [Abstract] [Full Text]  
• Uko, S., Soghier, L. M., Vega, M., Marsh, J., Reinersman, G. T., Herring, L., Dave, V. A., Nafday, S., Brion, L. P. (2006). Targeted Short-Term Fluconazole Prophylaxis Among Very Low Birth Weight and Extremely Low Birth Weight Infants. Pediatrics 117: 1243-1252 [Abstract] [Full Text]  
• Wiederhold, N. P., Kontoyiannis, D. P., Prince, R. A., Lewis, R. E. (2005). Attenuation of the Activity of Caspofungin at High Concentrations against Candida albicans: Possible Role of Cell Wall Integrity and Calcineurin Pathways. Antimicrob. Agents Chemother. 49: 5146-5148 [Abstract] [Full Text]  

This Article Abstract Full Text (PDF) Supplemental material Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liu, T. T. Articles by Rogers, P. D. Search for Related Content PubMed PubMed Citation Articles by Liu, T. T. Articles by Rogers, P. D.