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Antimicrobial Agents and Chemotherapy, October 2006, p. 3418-3423, Vol. 50, No. 10
0066-4804/06/$08.00+0     doi:10.1128/AAC.00241-06

Compartmentalized Intrapulmonary Pharmacokinetics of Amphotericin B and Its Lipid Formulations

Andreas H. Groll,^1,4 Caron A. Lyman,¹ Vidmantas Petraitis,¹ Ruta Petraitiene,¹ Derek Armstrong,¹ Diana Mickiene,¹ Raul M. Alfaro,² Robert L. Schaufele,¹ Tin Sein,¹ John Bacher,³ and Thomas J. Walsh^1*

Immunocompromised Host Section, Pediatric Oncology Branch, National Cancer Institute, Bethesda, Maryland,¹ Pharmacokinetics Research Laboratory, Pharmacy Department, Warren Grant Magnuson Clinical Center, Bethesda, Maryland,² Division of Veterinary Resources, National Institutes of Health, Bethesda, Maryland,³ Infectious Disease Research Program, Center for Bone Marrow Transplantation and Department of Pediatric Hematology/Oncology, University Children's Hospital, Muenster, Germany⁴

Received 24 February 2006/ Returned for modification 2 May 2006/ Accepted 18 June 2006

Top Abstract Introduction Materials and Methods Results Discussion References

 
We investigated the compartmentalized intrapulmonary pharmacokinetics of amphotericin B and its lipid formulations in healthy rabbits. Cohorts of three to seven noninfected, catheterized rabbits received 1 mg of amphotericin B deoxycholate (DAMB) per kg of body weight or 5 mg of either amphotericin B colloidal dispersion (ABCD), amphotericin B lipid complex (ABLC), or liposomal amphotericin B (LAMB) per kg once daily for a total of 8 days. Following sparse serial plasma sampling, rabbits were sacrificed 24 h after the last dose, and epithelial lining fluid (ELF), pulmonary alveolar macrophages (PAM), and lung tissue were obtained. Pharmacokinetic parameters in plasma were derived by model-independent techniques, and concentrations in ELF and PAM were calculated based on the urea dilution method and macrophage cell volume, respectively. Mean amphotericin B concentrations ± standard deviations (SD) in lung tissue and PAM were highest in ABLC-treated animals, exceeding concurrent plasma levels by 70- and 375-fold, respectively (in lung tissue, 16.24 ± 1.62 versus 2.71 ± 1.22, 6.29 ± 1.17, and 6.32 ± 0.57 µg/g for DAMB-, ABCD-, and LAMB-treated animals, respectively [P = 0.0029]; in PAM, 89.1 ± 37.0 versus 8.92 ± 2.89, 5.43 ± 1.75, and 7.52 ± 2.50 µg/ml for DAMB-, ABCD-, and LAMB-treated animals, respectively [P = 0.0246]). By comparison, drug concentrations in ELF were much lower than those achieved in lung tissue and PAM. Among the different cohorts, the highest ELF concentrations were found in LAMB-treated animals (2.28 ± 1.43 versus 0.44 ± 0.13, 0.68 ± 0.27, and 0.90 ± 0.28 µg/ml in DAMB-, ABCD-, and ABLC-treated animals, respectively [P = 0.0070]). In conclusion, amphotericin B and its lipid formulations displayed strikingly different patterns of disposition in lungs 24 h after dosing. Whereas the disposition of ABCD was overall not fundamentally different from that of DAMB, ABLC showed prominent accumulation in lung tissue and PAM, while LAMB achieved the highest concentrations in ELF.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Invasive mycoses of the lung caused by opportunistic filamentous fungi, such as Aspergillus spp., Zygomycetes, Fusarium spp., and Scedosporium spp., are frequent and often cause fatal infections in immunocompromised patients, particularly those with cancer and those who have undergone hematopoietic stem cell transplantation. Despite the emergence of novel antifungal triazoles and echinocandin lipopeptides, amphotericin B and its lipid formulations remain important options for empirical antifungal therapy for patients at the highest risk and for the treatment of probable and proven infections (13).

The four licensed formulations of amphotericin B have different physicochemical characteristics that result in distinct plasma pharmacokinetics (14, 27, 30). However, whether and to what extent these different dispositions of amphotericin B in plasma lead to different disposition patterns in distinct compartments of the lung are largely unknown.

The purposes of the present experimental studies, therefore, were to investigate the comparative intrapulmonary dispositions of the four licensed amphotericin B formulations in epithelial lining fluid (ELF), pulmonary alveolar macrophages (PAM), and total lung tissue and to relate these concentration data to their disposition in peripheral blood monocytes (PBM) and plasma.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Study drug. Deoxycholate amphotericin B (DAMB) was prepared from commercially available amphotericin B (Fungizone; Bristol-Myers Squibb, Princeton, NJ); the initial powder was dissolved with sterile water and then further diluted with 5% dextrose in water, as recommended, to a final concentration of 1 mg/ml. Amphotericin B colloidal dispersion (ABCD) (Amphotec; Intermune, Brisbane, CA) was provided as lyophilized sterile powder (100 mg/vial). Prior to its use, the powder was dissolved in 20 ml of sterile water and then further diluted with 5% dextrose in water to a final concentration of 1 mg/ml. Amphotericin B lipid complex (ABLC) (Abelcet; Enzon Pharmaceuticals, Bridgewater, NJ) was provided as a 5-mg/ml solution in 20-ml vials and further diluted to a 1-mg/ml solution with 5% dextrose in water prior to use. Liposomal amphotericin B (LAMB) (AmBisome; Fujisawa USA, Deerfield, IL) was prepared from lyophilized powder. The powder was initially reconstituted with 12 ml of sterile water, shaken vigorously for 30 seconds, filtered through a 5-µm filter, and further diluted with 5% dextrose in water to a final concentration of 1 mg/ml. All drugs were freshly prepared prior to use.

Animals. Healthy female New Zealand White rabbits (Oryctolagus cuniculus; Hazleton, Denver, PA) weighing 2.5 to 3.5 kg were used in all experiments. They were individually housed and maintained with water and standard autoclaved rabbit feed ad libitum according to National Institutes of Health (NIH) Guidelines for Laboratory Animal Care (18). Vascular access was established in each rabbit prior to experimentation by the surgical placement of a subcutaneous silastic central venous catheter under general anesthesia, as previously described (29).

Experimental design and dosage selection. Five groups of three to seven rabbits each were studied. Animals received either 1 mg/kg of body weight/day DAMB, 5 mg/kg/day ABCD, 5 mg/kg/day ABLC, 5 mg/kg/day LAMB, or 5% glucose in water over 5 min by a steady intravenous bolus once daily for a total of 8 days. On day 9 of the experiment, 24 h after the last dosage, rabbits were sacrificed by intravenously administered pentobarbital, following a blood drawing to harvest peripheral blood monocytes and to determine the urea nitrogen level in plasma. Immediately after the rabbits were sacrificed, postmortem examinations were performed, and lungs and intact tracheobronchial trees were aseptically removed.

Dosage selections for ABCD, ABLC, and LAMB were based on the results of previous infection models in rabbits, which have demonstrated equivalency to standard dosages of DAMB at 5 but not at 1 mg/kg/day (1, 10). These dosages also represent the standard dosages approved by the U.S. Food and Drug Administration (FDA) for the treatment of invasive fungal infections in humans (2).

Plasma pharmacokinetics. Following the last dose, a minimal sampling strategy was used to obtain both the peak concentration (C_max) and the trough concentration (C_min) in plasma, the area under the concentration-time curve from 0 to 24 h (AUC_0-24), the apparent volume of distribution at steady state (V_ss), and the total plasma clearance (CL_t) rate. This approach allowed for the direct determination of pharmacokinetic parameters in each animal while minimizing perturbation of the experiment caused by excessive blood loss. Time points for minimal plasma sampling were determined from full plasma concentration-time profiles of healthy rabbits. Plasma sampled immediately before and after administration of the drug and then at 1, 6, 12, and 24 h postdosing was found to reflect full plasma concentration-time profiles of all four formulations (12).

BAL. Lungs and intact bronchoalveolar trees were carefully dissected and aseptically removed in toto for bronchoalveolar lavage (BAL) and harvest of PAM and lung tissue. BAL was performed on each lung preparation by the instillation and subsequent aspiration of 10 ml of sterile normal saline two times into the clamped trachea with a sterile 12-ml syringe. The aspirated volume was recorded, and specimens were centrifuged immediately for 10 min at 2,000 x g. The supernatant was separated from the cell pellet and stored at –80°C until assayed.

Harvest of pulmonary alveolar macrophages and lung tissue. Following bronchoalveolar lavage, the edges of the pulmonary lobes were cut and 20 ml of phosphate-buffered saline was forcefully injected into each lobe three to four times per lobe (24). The lavage washings, rich in pulmonary alveolar cells, were collected, filtered through sterile gauze pads, and pelleted at 400 x g at 4°C for 10 min. The supernatant was removed, and the pellets were resuspended in Hanks ' balanced salt solution free of Ca²⁺ and Mg²⁺. Contaminating erythrocytes were lysed in distilled water. More than 95% of the purified cells were macrophages by morphology and nonspecific esterase staining. The yield of PAM from each rabbit ranged from 30 x 10⁶ to 90 x 10⁶ cells. Pulmonary alveolar macrophages were sonicated three times for 20 s each with a sonic Dismembranator (Artec Systems Corp., Farmingdale, NY) set at the 35% cycle; the microtip sonic transducer was used, and samples were placed on ice and then stored at –80°C until assayed. After the completion of the pulmonary alveolar tissue harvest, the remaining buffer solution was blotted from lung tissue with Micro Wipes (Scott Paper Company, Philadelphia, PA), and lung tissue was stored at –80°C until assayed.

Harvest of peripheral blood monocytes. Thirty milliliters of blood was obtained from each animal immediately prior to sacrifice. PBM were separated from blood buffy coats by centrifugation over Ficoll as previously described (23). Cells were resuspended in Hanks' balanced salt solution free of Ca²⁺ and Mg²⁺and counted. The yield of PBM from each rabbit ranged from 35 x 10⁶ to 166 x 10⁶ cells. PBM were sonicated three times for 20 s each with a sonic Dismembranator (Artec Systems Corp., Farmingdale, NY) set at the 35% cycle; the microtip sonic transducer was used, and samples were placed on ice and then stored at –80°C until assayed.

Determination of ELF volumes and concentrations of amphotericin B in ELF. As urea diffuses freely throughout body fluids, the volume of ELF can be deduced from the dilution of urea in BAL fluid (22). The concentrations of urea in sera were analyzed by the Clinical Chemistry Laboratory of the Clinical Center of the NIH by a coupled urease-glutamate dehydrogenase method modified by Roche Diagnostics (Indianapolis, IN). Urea in the BAL fluid supernatant was measured by a modified enzymatic assay (blood urea nitrogen kit 640A; Sigma, St. Louis, MO) read with an Ultraspec 2000 spectrophotometer (Pharmacia Biotech, Cambridge, United Kingdom). The proportions of reagent to specimen were changed from 3.0 ml/0.005 ml to 2.5 ml/0.5 ml, as recommended by the manufacturer, as reported by Rennard et al. (22). A standard curve for normal saline ranging from 0.046 to 1.5 µl was used and was linear (r², 1.000). Accuracy and precision were within 10%, as assessed by separately prepared quality control samples (0.093 and 0.750 µg/ml, respectively), run in triplicate with standard curve and supernatants.

The volume of ELF in BAL fluid was derived from the following relationship: V_ELF = V_BAL x (urea_BAL/urea_ser), where V_ELF is the volume of ELF sampled by BAL, V_BAL is the volume of aspirated BAL fluid, and urea_ser is the concentra- tion of urea in serum.

The concentration of amphotericin B in epithelial lining fluid (C_ELF) was derived by the following equation: C_ELF = C_BAL x (V_BAL/V_ELF), where C_BAL is the measured amphotericin B concentration in the BAL fluid supernatant (9).

Determination of concentrations of amphotericin B in PAM and PBM. The total volumes of PAM and PBM were determined from the absolute cell counts by assuming a mean monocyte/macrophage cell volume of 2.42 µl/10⁶ cells (5). The concentrations of amphotericin B in PAM and PBM (C_PAM and C_PBM, respectively) was derived by the following equation: C_PAM or C_PBM = (AMB_cells/V_cells), where AMB_cells is the amphotericin B concentration in 1 ml of suspension, and V_cells is the volume of pulmonary alveolar macrophages in the 1-ml suspension (9).

Processing and preparation of analytical samples. Blood samples for analytical purposes were collected in heparinized syringes, and plasma was separated by centrifugation. Like the BAL fluid, pulmonary alveolar macrophage, lung tissue, and plasma samples were stored at –80°C until assayed.

Extraction of amphotericin B was achieved by the precipitation of proteins using a 1:2 (vol/vol) ratio of methanol to plasma and a 1:1 (vol/vol) ratio of methanol to BAL fluid or pulmonary alveolar macrophages. This was followed by incubation for 30 min at 4°C, centrifugation at 2,000 x g for 10 min, transfer of the methanolic supernatant into a 0.22-µm Durapore filter tube, and centrifugation at 4,000 x g for 4 min for injection. Standards and quality controls were prepared similarly from pooled normal rabbit serum (Gibco Laboratories, Grand Islands, NY) or Hanks' balanced solution without Ca²⁺, Mg²⁺, and phenol red (NIH Media Unit, Bethesda, MD). For the extraction of amphotericin B from lung tissue, an aliquot of approximately 1 g of tissue was homogenized directly with ice-cold methanol (1:2 [wt/wt]) in a high-speed tissue homogenizer (Tizzumizer; Tekmar, Cincinnati, OH) two times for 20 s each. Homogenized lung tissue samples were incubated for 30 min at 4°C and centrifuged at 2,000 x g for 10 min. The methanolic supernatant was transferred into a 0.22-µm Durapore filter tube and centrifuged at 4,000 x g for 4 min for injection. Standards and quality control samples were prepared similarly by adding known amounts of amphotericin B to drug-free lung homogenates. Amphotericin B concentrations in lung tissue were calculated to 1 g of tissue. For all matrices, blank samples also were assayed to ensure the absence of interfering peaks.

Analytical method. Concentrations of all four investigated formulations were determined as concentrations of amphotericin B by an internally validated, modified reversed-phase high-performance liquid chromatographic method (12). Commercially available amphotericin B powder (Sigma, St. Louis, MO) was used as a reference standard.

The mobile phase was methanol-acetonitrile-0.0025 M Na-EDTA (500:350:200), delivered at 1.6 ml/min. The injection volume ranged from 100 µl (plasma and lung homogenates) to 200 µl (BAL fluid and pulmonary alveolar macrophages). Amphotericin B was eluted at a rate of 3.5 to 4.5 min using a C₁₈ analytical column (Waters, Milford, MA) preceded by a NewGuard C₁₈ precolumn (Perkin Elmer, Norwalk, CT) and was detected at 382 nm by UV absorbance.

Quantitation was based on the peak-height concentration response of the external calibration standard. Eight- to 12-point standard curves (0 to 5.0 µg/ml and 0 to 50 µg/ml for plasma and 0 to 10 µg/ml for lung homogenate and Hanks' balanced salt solution) were linear, with R² values greater than 0.990. The lower limits of quantitation were 0.040 µg/ml for plasma, 0.12 µg/g for lung tissue, and 0.010 µg/ml for Hanks' balanced salt solution. Accuracies were within ±10%, and intra- and interday variability values (precision) were <7.0% for all matrices.

Pharmacokinetic parameters. Peak plasma concentrations (C_max) and concentrations 24 h after dosing (C_min) were obtained directly from plasma concentration-time profiles. The plasma AUC_0-24, the apparent V_ss, and the plasma CL_t were derived using standard noncompartmental techniques (11). Pharmacokinetic parameters included plasma C_max, C_min, AUC_0-24, V_ss, CL_t, C_ELF, C_PAM, and concentration in lung tissue (C_tissue) 24 h after dosing.

Statistical considerations. All values are presented as the means ± standard deviations (SD) from three to seven animals. Differences between the means of continuous data were evaluated by Kruskal-Wallis nonparametric analysis of variance (ANOVA) with Dunn's correction for multiple comparisons, as appropriate. A two-tailed P value of 0.05 was considered statistically significant.

Top Abstract Introduction Materials and Methods Results Discussion References

 
Plasma pharmacokinetics. The observed plasma concentration-time profiles of amphotericin B following once daily administration of either DAMB, ABCD, ABLC, or LAMB over 8 days are shown in Fig. 1, and the corresponding mean noncompartmental pharmacokinetic parameters are listed in Table 1. A comparison of the dispositions of amphotericin B in plasma revealed marked differences among the four compounds. Repeat dosing with 5 mg/kg of ABCD achieved peak and trough concentrations and AUC_0-24 values similar to those of DAMB administered at the standard dosage of 1 mg/kg; the mean clearance rate of ABCD was faster and the mean V_ss was larger than those of DAMB. ABLC administered at 5 mg/kg was more rapidly eliminated from plasma and was associated with a larger V_ss and lower peak and trough concentrations as well as lower AUC_0-24 values than those of both DAMB and ABCD. In contrast to dosing with DAMB, ABCD, and ABLC, dosing with LAMB at 5 mg/kg resulted in a prolonged circulation of amphotericin B in plasma, with slow elimination and escalated peak and trough concentrations and AUC_0-24 values.

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FIG. 1. Concentration-time profiles of total amphotericin B in plasma following once-daily dosing with either DAMB, ABCD, ABLC, or LAMB for 8 days. Each point plots the mean concentration ± SD from three to seven rabbits at the indicated time.

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TABLE 1. Pharmacokinetic parameters of DAMB, ABCD, ABLC, and LAMB in plasma following once-daily dosing for 8 daysa

Intrapulmonary disposition. Mean concentrations of amphotericin B at 24 h following the last of eight daily dosages of either DAMB, ABCD, ABLC, or LAMB in lung tissue, ELF, PAM, and, for comparison, PBMs and concurrent plasma samples are listed in Table 2. Concentrations of amphotericin B in lung tissue and PAM were highest in the ABLC-treated animals. Drug concentrations in ELF were generally much lower than those achieved in lung tissue and PAM. Among the different cohorts, the highest absolute ELF concentrations were found in LAMB-treated animals (Table 2).

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TABLE 2. Concentrations of amphotericin B in lung tissue, ELF, PAMs, and PBMs after once-daily dosing for 8 daysa

The mean site-to-plasma concentration ratios of amphotericin B at 24 h following the last of eight daily dosages are depicted in Fig. 2. Amphotericin B concentrations in lung tissue and PAM exceeded concurrent plasma levels severalfold in DAMB-, ABCD-, and ABLC-treated animals; the largest accumulation in these sites was found in the cohort of ABLC-treated animals, with mean (±SD) site-to-plasma ratios of 70 ± 20 and 375 ± 124, respectively. In contrast, the mean site-to-plasma ratios of amphotericin B in lung tissue and PAM in LAMB-treated animals were well below 1. Mean concentrations of amphotericin in ELF were similar or slightly higher than concurrent plasma levels in DAMB-, ABCD-, and ABLC-treated animals but approximately 10-fold lower in LAMB-treated animals.

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FIG. 2. Intrapulmonary site-to-plasma concentration ratios of amphotericin B in lung tissue, ELF, and PAM at 24 h after the last of eight daily dosages of either DAMB, ABCD, ABLC, or LAMB. Each bar represents the mean value from three to seven rabbits ± the SD. Tissue, lung tissue. *, P = 0.0019 by ANOVA (<0.05 for ABCD and ABLC, respectively, versus LAMB); **, P = 0.0076 by ANOVA (<0.05 for ABCD and ABLC, respectively, versus LAMB); ***, P = 0.0026 (<0.05 for DAMB and ABLC, respectively, versus LAMB).

Disposition in peripheral blood monocytes. Mean concentrations of amphotericin B associated with PBM appeared to be highest in the ABCD-treated animals and lowest in the ABLC-treated animals (Table 2). Concentrations of amphotericin B associated with PBMs were generally severalfold lower than those associated with PAM, most notably in ABLC-treated animals. However, with the exception of LAMB-treated animals, comparisons with concurrent plasma concentration values were indicative of accumulation in or with PBM (mean site-to-plasma ratios ± standard errors of the means, 3.21 ± 1.70, 9.21 ± 9.25, 4.19 ± 3.80, and 0.06 ± 0.03 for DAMB-, ABCD-, ABLC-, and LAMB-treated animals, respectively [P = 0.0387 by ANOVA]).

Top Abstract Introduction Materials and Methods Results Discussion References

 
The results of these experimental pharmacokinetic studies demonstrate that therapeutic dosages of the four licensed amphotericin B formulations display markedly different plasma pharmacokinetics that, in turn, are associated with distinct disposition patterns in the lungs after multiple dosing. While the disposition of ABCD in plasma was similar to that of DAMB, ABLC was rapidly eliminated from the bloodstream and achieved comparatively lower plasma C_max and AUC_0-24 values. In contrast, LAMB showed a prolonged circulation in blood, with slow elimination and escalating C_max and AUC_0-24 values. Twenty-four hours after the last of eight daily dosages, mean amphotericin B concentrations in lung tissue and PAM were highest in ABLC-treated animals, exceeding concurrent plasma levels 70- and 375-fold, respectively. Mean drug concentrations in ELF were generally much lower than those achieved in lung tissue and PAM; among the different cohorts, the highest ELF concentrations were found in LAMB-treated animals. There was no apparent direct correlation between plasma and intrapulmonary drug concentrations, which is indicative of the pivotal importance of the carrier for the intrapulmonary disposition of amphotericin B.

As plasma concentrations may not necessarily predict effective concentrations at the target site, tissue penetration studies have become an integral part of the pharmacokinetic assessment of antimicrobial agents. For example, in the setting of community-acquired respiratory infections, compartmental intrapulmonary distributions and their relationship to MICs provide the scientific rationale for the use and dosing regimens of macrolides, azalides, and quinolones (3, 19). We therefore sought to further understand the distribution of amphotericin B in the lung by analyzing its distribution into PAM, ELF, and total lung tissue.

The four licensed amphotericin B formulations possess distinct physicochemical and pharmacokinetic properties. As an amphiphilic molecule that lacks significant metabolism and rapid elimination from the body, amphotericin B is preferentially taken up by cells of the mononuclear phagocytic system (7). Amphotericin B administered as ABCD and ABLC is rapidly taken up into the mononuclear phagocytic system, which is reflected by lower peak plasma concentrations and AUC values and a larger volume of distribution than those of DAMB. In contrast, amphotericin B administered as LAMB has a prolonged circulation in the bloodstream, with strikingly high peak plasma concentrations and AUC values (6, 14, 28).

Whether and how these distinct physicochemical and pharmacokinetic features translate into different pharmacodynamic properties in the lungs are largely unknown. In single- and multidose distribution studies of rodents that used equimolar dosages of 1 mg/kg of the parent drug, concentrations in whole-lung homogenates achieved with ABCD and LAMB were lower and those achieved with ABLC were similar or slightly higher than those achieved with DAMB as the reference agent. After multiple dosing of 5-fold (ABCD and LAMB)- to 10-fold (ABLC)-higher dosages, drug accumulation in the lungs clearly exceeded that achieved by 1 mg/kg of DAMB (20, 21, 28). These differences in lung distribution appear to correspond to the results of comparative pulmonary infection models, where the lipid formulations required at least fivefold-higher dosages to produce a reduction of the residual fungal burden in lung tissue equivalent or superior to that produced by standard dosages of DAMB (1, 8, 10).

The carrier effects of the different amphotericin B formulations suggest that compartmental lung tissue concentrations may indeed be the more relevant pharmacokinetic parameters to be correlated with antifungal efficacy. This question, however, has been addressed only in very select aspects. Koizumi and coworkers investigated the distribution of amphotericin B in lung lymph following intravenous administration of 1 mg/kg of DAMB to adult sheep with lung lymph fistulas. While the lung lymph-to-plasma ratio of amphotericin B increased gradually during infusion and was more than 1 after the end of the infusion, the AUC_0-24s in plasma and lung lymph were virtually identical, suggesting that amphotericin B could easily move from plasma to the pulmonary interstitium and/or the lung lymph circulation (16). In spontaneously breathing, anesthetized cats, Matot and Pizov studied the single-dose uptake of 1 mg/kg DAMB, ABLC, and LAMB by intact lungs using double-indicator outflow curves. While the mean rates of first-pass uptake of all three formulations were similar, at 69 to 82%, ABLC exhibited prolonged retention in the lungs: 23 and 15% of the injected dose remained in the lungs for 1 and 6 h after administration, respectively, compared to 2% after 1 h in DAMB- and LAMB-treated animals (17). This prolonged retention of ABLC in lung tissue is consistent with our findings of highly elevated concentrations predominantly in PAM.

The data reported herein provide the first evidence for a distinct intrapulmonary disposition pattern of currently licensed amphotericin B formulations. As the plasma pharmacokinetics of all four formulations in the rabbit are similar to those in humans (12) and since fundamental structural or functional differences of the anatomical barriers between blood and airspace do not exist between the two species, the results are principally suitable for inference to patients.

The anatomic sites of airborne pulmonary mold infections are primarily extravascular and include the distal airways and the alveoli. PAM constitute an important additional compartment, as they are abundant and constitute the first line of phagocytic host defense (25). Conceptually, pulmonary mold infections would thus require the delivery of a bioactive drug in therapeutic concentrations to these extravascular sites of infection and intracellular compartments of pulmonary macrophages.

While the exact mechanisms of drug penetration of amphotericin into the lungs remain unclear, because of their size, the carriers themselves are unlikely to pass through the nonfenestrated capillary endothelium, the capillary basement membrane, the alveolar basement membrane, and alveolar pneumocytes into the alveolar space. Instead, amphotericin B may leave the pulmonary microcirculation and enter the alveolar space by several different mechanisms: diffusion of free drug along a concentration gradient (permeation), uptake of bound and unbound drug from blood into PAM with subsequent migration into the alveolar compartment or infected tissue, uptake of lipoprotein-bound amphotericin B via endothelial lipoprotein receptors, and pinocytosis of amphotericin B by endothelial cells (15, 19, 26, 31). Whereas additional drug disposition may result from the leakage of bound and free drug into tissue when anatomic barriers are damaged by infection, inflammation, or necrosis (15, 19, 26), these proposed mechanisms do not fully account for the capacity of these large molecules to traverse the complex cellular and connective tissue barriers that separate pulmonary capillaries and alveolar spaces, particularly in noninfected lung tissue.

The differential intrapulmonary dispositions and the lack of a direct correlation between plasma- and intrapulmonary drug concentrations in this study are indicative of the pivotal importance of the carrier for the transfer of amphotericin B into different compartments of healthy lungs. At their respective therapeutic dosages, the two small, variably stable micellar formulations, DAMB and ABCD, displayed similar plasma pharmacokinetics and similar intrapulmonary disposition patterns, with considerable drug disposition into PAM and lung tissue, but with comparatively low drug concentrations in ELF. The small, stable unilamellar liposomal formulation, despite producing a severalfold increase in drug exposure in the bloodstream during the dosing interval, achieved drug concentrations in PAM and lung tissue similar to those of DAMB and ABCD but the highest ELF concentrations among all compounds, which may be explained by the relatively higher drug concentration gradient across the barrier between blood and alveolar space. ABLC, in contrast, preferentially distributed to PAM; it is conceivable that the comparatively high lung tissue concentrations are caused by the fraction of PAM that remained tissue bound after the lavage procedure. Consistent with the observations of Matot and Pizov (17), the large ribbon-like phospholipids may be trapped in the capillary bed of the lung and amphotericin B may be phagocytosed either with its carrier or following its degradation by phospholipids. The prolonged retention of ABLC in the pulmonary capillaries may thus permit a greater duration of time for uptake by PAM.

It is unknown, nevertheless, whether achieving a concentration equilibrium among PAM, lung tissue, and ELF or preferential dispositions in PAM and lung tissue is of therapeutic advantage. In a recent comparative pharmacokinetic/pharmacodynamic study of neutropenic mice intravenously infected with Candida albicans (4), amphotericin B concentrations in the lungs were highest following ABLC administration, and these enhanced concentrations correlated with enhanced potency at this site, as expressed by the 75% effective dose. However, findings from a disseminated blood-borne yeast infection may not be translated directly to the setting of airborne pulmonary mold infections that follow different patterns of infection and lung tissue damage.

In conclusion, therapeutic dosages of the four currently approved amphotericin B formulations displayed markedly different plasma pharmacokinetics that were associated with distinct disposition patterns in the lung after multiple doses. Whereas the intrapulmonary disposition of ABCD was overall not fundamentally different from that of DAMB, ABLC showed prominent accumulation in lung tissue, and PAM and LAMB achieved the highest concentrations in ELF.

 
This research was supported in part by the Intramural Research Program of the National Institutes of Health and the National Cancer Institute.

 
* Corresponding author. Mailing address: CRC-1-5750, 10 Center Drive, Bethesda, MD 20892. Phone: (301) 402-0023. Fax: (301) 480-2308. E-mail: walsht{at}mail.nih.gov.

Top Abstract Introduction Materials and Methods Results Discussion References

 

1
1. Allende, M. C., J. W. Lee, P. Francis, K. Garrett, H. Dollenberg, J. Berenguer, C. A. Lyman, P. A. Pizzo, and T. J. Walsh. 1994. Dose-dependent antifungal activity and nephrotoxicity of amphotericin B colloidal dispersion in experimental pulmonary aspergillosis. Antimicrob. Agents Chemother. 38:518-522.[Abstract/Free Full Text]
2
2. American Hospital Formulary Service Drug Information. 1999. Antifungal antibiotics, p. 74-85. In AHFS drug information. American Society of Health System Pharmacists, Bethesda, Md.
3
3. Andes, D., J. Anon, M. R. Jacobs, and W. A. Craig. 2004. Application of pharmacokinetics and pharmacodynamics to antimicrobial therapy of respiratory tract infections. Clin. Lab. Med. 24:477-502.[CrossRef][Medline]
4
4. Andes, D., N. Safdar, K. Marchillo, and R. Conklin. 2006. Pharmacokinetic-pharmacodynamic comparison of amphotericin B (AMB) and two lipid-associated AMB preparations, liposomal AMB and AMB lipid complex, in murine candidiasis models. Antimicrob. Agents Chemother. 50:674-684.[Abstract/Free Full Text]
5
5. Baldwin, D. R., R. Wise, J. M. Andrews, J. P. Ashby, and D. Honeybourne. 1990. Azithromycin concentrations at the site of pulmonary infection. Eur. Respir. J. 3:886-890.[Abstract]
6
6. Bekersky, I., R. M. Fielding, D. E. Dressler, J. W. Lee, D. N. Buell, and T. J. Walsh. 2002. Pharmacokinetics, excretion, and mass balance of liposomal amphotericin B (AmBisome) and amphotericin B deoxycholate in humans. Antimicrob. Agents Chemother. 46:828-833.[Abstract/Free Full Text]
7
7. Brajtburg, J., and J. Bolard. 1996. Carrier effects on biological activity of amphotericin B. Clin. Microbiol. Rev. 9:512-531.[Abstract]
8
8. Clemons, K. V., and D. A. Stevens. 1993. Therapeutic efficacy of a liposomal formulation of amphotericin B (AmBisome) against murine blastomycosis. J. Antimicrob. Chemother. 32:465-472.[Abstract/Free Full Text]
9
9. Conte, J. E., Jr., J. A. Golden, S. Duncan, E. McKenna, and E. Zurlinden. 1995. Intrapulmonary pharmacokinetics of clarithromycin and of erythromycin. Antimicrob. Agents Chemother. 39:334-338.[Abstract/Free Full Text]
10
10. Francis, P., J. W. Lee, A. Hoffman, J. Peter, A. Francesconi, J. S. Bacher, J. Shelhamer, P. A. Pizzo, and T. J. Walsh. 1994. Efficacy of unilamellar liposomal amphotericin B in treatment of pulmonary aspergillosis in persistently granulocytopenic rabbits: the potential role of bronchoalveolar D-mannitol and serum galactomannan as markers of infection. J. Infect. Dis. 169:356-368.[Medline]
11
11. Gibaldi, M., and D. Perrier. 1982. Pharmacokinetics, 2nd ed., p. 455-459. Marcel Dekker, New York, N.Y.
12
12. Groll, A. H., N. Giri, V. Petraitis, R. Petraitiene, M. Candelario, J. S. Bacher, S. C. Piscitelli, and T. J. Walsh. 2000. Comparative efficacy and distribution of lipid formulations of amphotericin B in experimental Candida albicans infection of the central nervous system. J. Infect. Dis. 182:274-282.[CrossRef][Medline]
13
13. Groll, A. H., and T. J. Walsh. 2002. Antifungal chemotherapy: advances and perspectives. Swiss Med. Wkly. 132:303-311.[Medline]
14
14. Groll, A. H., J. C. Gea-Banacloche, A. Glasmacher, G. Just-Nuebling, G. Maschmeyer, and T. J. Walsh. 2003. Clinical pharmacology of antifungal compounds. Infect. Dis. Clin. N. Am. 17:159-191.[CrossRef][Medline]
15
15. Honeybourne, D., and D. R. Baldwin. 1992. The site concentrations of antimicrobial agents in the lung. J. Antimicrob. Chemother. 30:249-260.[Free Full Text]
16
16. Koizumi, T., K. Kubo, T. Kaneki, M. Hanaoka, T. Hayano, T. Miyahara, K. Okada, K. Fujimoto, H. Yamamoto, T. Kobayashi, and M. Sekiguchi. 1998. Pharmacokinetic evaluation of amphotericin B in lung tissue: lung lymph distribution after intravenous injection and airspace distribution after aerosolization and inhalation of amphotericin B. Antimicrob. Agents Chemother. 42:1597-1600.[Abstract/Free Full Text]
17
17. Matot, I., and R. Pizov. 2000. Pulmonary extraction and accumulation of lipid formulations of amphotericin B. Crit. Care Med. 28:2528-2532.[CrossRef][Medline]
18
18. National Research Council Committee on the Care and Use of Laboratory Animals, Institute of Laboratory Animal Resources, Commission on Life Sciences. 1996. Guide for the care and use of laboratory animals. National Academy Press, Washington, D.C.
19
19. Nix, D. E. 1998. Intrapulmonary concentrations of antimicrobial agents. Infect. Dis. Clin. N. Am. 12:631-646.[Medline]
20
20. Olsen, S. J., M. R. Swerdel, B. Blue, J. M. Clark, and D. P. Bonner. 1991. Tissue distribution of amphotericin B lipid complex in laboratory animals. J. Pharm. Pharmacol. 43:831-835.[Medline]
21
21. Proffitt, R. T., A. Sartorius, S. M. Chiang, L. Sullivan, and J. P. Adler-Moore. 1991. Pharmacology and toxicology of a liposomal formulation of amphotericin B (AmBisome) in rodents. J. Antimicrob. Chemother. 28(Suppl. B):49-61.
22
22. Rennard, S. I., G. Basset, D. Lecossier, K. M. O'Donnell, P. Pinkston, P. G. Martin, and R. G. Crystal. 1986. Estimation of volume of epithelial lining fluid recovered by lavage using urea as marker of dilution. J. Appl. Physiol. 60:532-538.[Abstract/Free Full Text]
23
23. Roilides, E., A. Dimitriadou, I. Kadiltsoglou, T. Sein, J. Karpouzas, P. A. Pizzo, and T. J. Walsh. 1997. IL-10 exerts suppressive and enhancing effects on antifungal activity of mononuclear phagocytes against Aspergillus fumigatus. J. Immunol. 158:322-329.[Abstract]
24
24. Roilides, E., C. A. Lyman, T. Sein, C. Gonzalez, and T. J. Walsh. 2000. Antifungal activity of splenic, liver and pulmonary macrophages against Candida albicans and effects of macrophage colony-stimulating factor. Med. Mycol. 38:161-168.[Medline]
25
25. Schaffner, A., H. Douglas, and A. Braude. 1982. Selective protection against conidia by mononuclear and against mycelia by polymorphonuclear phagocytes in resistance to Aspergillus: observations on these two lines of defense in vivo and in vitro with human and mouse phagocytes. J. Clin. Investig. 69:617-631.[Medline]
26
26. Schentag, J. J., and C. H. Ballow. 1991. Tissue-directed pharmacokinetics. Am. J. Med. 91(Suppl. 3A):5S-11S.[Medline]
27
27. Storm, G., and E. van Etten. 1997. Biopharmaceutical aspects of lipid formulations of amphotericin B. Eur. J. Clin. Microbiol. Infect. Dis. 10:64-73.
28
28. Walsh, T. J., A. J. Jackson, J. W. Lee, M. Amantea, T. Sein, J. Bacher, and L. Zech. 2000. Dose-dependent pharmacokinetics of amphotericin B lipid complex in rabbits. Antimicrob. Agents Chemother. 44:2068-2076.[Abstract/Free Full Text]
29
29. Walsh, T. J., P. Bacher, and P. A. Pizzo. 1988. Chronic silastic central venous catheterization for induction, maintenance, and support of persistent granulocytopenia in rabbits. Lab. Anim. Sci. 38:467-471.[Medline]
30
30. Wang, L. H., R. M. Fielding, P. C. Smith, and L. S. S. Guo. 1995. Comparative tissue distribution and elimination of amphotericin B colloidal dispersion (Amphocil) and Fungizone after repeated dosing in rats. Pharm. Res. 12:275-283.[CrossRef][Medline]
31
31. Wasan, K. M., A. L. Kennedy, S. M. Cassidy, M. Ramaswamy, L. Holtorf, J. W.-L. Chou, and P. H. Pritchard. 1998. Pharmacokinetics, distribution in serum lipoproteins and tissues, and renal toxicities of amphotericin B and amphotericin B lipid complex in a hypercholesterolemic rabbit model: single-dose studies. Antimicrob. Agents Chemother. 42:3146-3152.[Abstract/Free Full Text]

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Antimicrobial Agents and Chemotherapy, October 2006, p. 3418-3423, Vol. 50, No. 10
0066-4804/06/$08.00+0     doi:10.1128/AAC.00241-06

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