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Antimicrobial Agents and Chemotherapy, November 2006, p. 3920-3922, Vol. 50, No. 11
0066-4804/06/$08.00+0     doi:10.1128/AAC.00223-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Novel Streptomycin Resistance Gene from Mycobacterium fortuitum{triangledown}

Santiago Ramón-García, Isabel Otal, Carlos Martín, Rafael Gómez-Lus, and José A. Aínsa*

Departamento de Microbiología, Medicina Preventiva y Salud Pública, Facultad de Medicina, Universidad de Zaragoza, 50009 Zaragoza, Spain

Received 21 February 2006/ Returned for modification 2 June 2006/ Accepted 30 August 2006


   ABSTRACT
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We have isolated the aph(3")-Ic gene, encoding an aminoglycoside 3"-O-phosphotransferase [APH(3")-Ic], from a genomic library of an environmental Mycobacterium fortuitum strain, selecting for streptomycin resistance. APH(3")-Ic phosphorylates and inactivates streptomycin. Similar genes have been described in Streptomyces griseus and plasmid RSF1010. It is also present in some M. fortuitum clinical isolates.


   TEXT
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Streptomycin (STR) resistance in Mycobacterium, a genus that includes important human pathogens, such as Mycobacterium tuberculosis, along with fast-growing opportunistic species, such as Mycobacterium fortuitum, is due to several mechanisms. Mutations in either the S12 ribosomal protein or the 16S rRNA confer high and moderate levels of STR resistance (21). We have previously characterized an efflux pump from M. fortuitum and M. tuberculosis that transports and confers decreased susceptibility to STR (1). In the present work, we describe the isolation and characterization of a novel aminoglycoside phosphotransferase gene from M. fortuitum which can confer STR resistance in mycobacteria.

Isolation of the M. fortuitum aph(3")-Ic gene. We constructed a plasmid library of genomic DNA from M. fortuitum strain FC1, a natural isolate which shows resistance to 50 µg/ml of STR (2), and transformed the library into M. smegmatis mc2155 (16) (STR MIC, 0.5 µg/ml) by electroporation as described previously (13). Six colonies were selected that showed a 64-fold increase in the STR MIC, as determined by the microtiter assay using resazurin (12), whereas transformation of vector pSUM36 (2) did not produced such an increase. Among the recombinant plasmids from STR-resistant colonies, pAC5 and pAC6 were found, and they overlapped in a common 2.5-kb fragment (Fig. 1). This fragment was subcloned in pSUM36, resulting in pSAN19, which was also capable of increasing the STR MIC for M. smegmatis mc2155 (Table 1).


Figure 1
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  		FIG. 1. Open reading frames present in the cloned fragments conferring STR resistance from M. fortuitum FC1 and comparison with a similar locus in M. smegmatis mc2155. orfA, orfB, and orfE are found in the loci of both M. fortuitum and M. smegmatis; orfC and orfD are found only in the locus of M. fortuitum.

 

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  		TABLE 1. MICs of aminoglycosides for M. smegmatis and M. bovis BCG strains containing or lacking the aph(3")-Ic gene

 
Sequencing and FramePlot analysis (http://www.nih.go.jp/~jun/cgi-bin/frameplot.pl) (10) revealed three complete and two incomplete open reading frames (Fig. 1). orfA and orfB are similar to Rv0513 and Rv0514 from M. tuberculosis (possible transmembrane proteins; 65.4 and 46% amino acid identity, respectively). orfC is similar to aph(3")-Ia from Streptomyces griseus and aph(3")-Ib from plasmid RSF1010 (streptomycin phosphotransferases; 53.3 and 46.5% amino acid identity, respectively). orfD is similar to Rv0519c (possible conserved membrane protein; 56.5% amino acid identity) and Rv0774c (possible conserved exported protein; 53.4% amino acid identity) from M. tuberculosis; orfE is similar to Rv0826 and Rv1645c from M. tuberculosis (conserved hypothetical proteins; 37.8 and 44.7% amino acid identity, respectively).

Since the protein encoded by orfC shows similarity to APH(3") enzymes, it is putatively involved in the STR resistance phenotype. A 1-kb fragment containing only orfC (plasmid pSAN26; Fig. 1) also conferred STR resistance to M. smegmatis mc2155 (Table 1). Introduction of pSAN19 or pSAN26 by electroporation into slow-growing M. bovis BCG-Pasteur conferred a 16-fold increase in the STR MICs (Table 1). Plasmid pSAN26 did not confer any increase in the MICs of the aminoglycosides spectinomycin, gentamicin, amikacin, 2'-N-ethylnetilmicin, and 6'-N-ethylnetilmicin to M. smegmatis mc2155 and M. bovis BCG-Pasteur (Table 1); therefore, STR seems to be the only substrate of the APH(3")-like enzyme from M. fortuitum FC1.

As mentioned above, the closest relatives of the APH(3")-like enzyme from M. fortuitum FC1 are the APH(3")-Ia enzyme from STR-producing S. griseus strains (8, 15, 17) and APH(3")-Ib, encoded by the broad-host-range plasmid RSF1010 (19) (Fig. 2). According to Shaw et al. (14), the enzyme from M. fortuitum was named APH(3")-Ic.


Figure 2
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  		FIG. 2. Comparison of APH(3") enzymes from S. griseus, M. fortuitum, and plasmid RSF1010. The motif HGD(X)4N, characteristic of this family of proteins (20), has been located within the histidine domain II between residues 172 and 179 of the mycobacterial APH(3"). Other catalytic motifs described by Wright and Thompson (20) and Heinzel et al. (8) are boxed, and the significant residues are highlighted in black.

 
Enzymatic activity of the APH(3")-Ic enzyme. Previously, we reported STR phosphotransferase activity in some M. fortuitum isolates (R. Gómez-Lus, J. Timm, C. Martín, J. Davies, and B. Gicquel, Abstr. 29th Intersci. Conf. Antimicrob. Agents Chemother., abstr. 156, 1989). Since the antimicrobial activity of STR is lost when isolates are phosphorylated by APH(3") enzymes, we tested the ability of crude extracts of M. smegmatis strains [producing or not producing APH(3")-Ic] to inactivate STR. For this, we first obtained crude extracts from M. smegmatis strains by sonication (Bioruptor; Cosmo Bio Co., Ltd.). Next, 4 mg/ml of protein from the crude extracts was mixed with ATP and STR as described previously (7). After 1, 2, and 4 h of incubation at 37°C, 5 µl from each reaction was taken in triplicate, added to paper discs, and dried. Finally, discs were assayed against STR-susceptible Escherichia coli ATCC 25922. We estimated the load of STR on the discs by comparing the size of the inhibition zones with that of a series of discs containing known amounts of STR.

No loss of antimicrobial activity of STR was observed when crude extracts of either M. smegmatis mc2155 (wild type) or M. smegmatis strain SUM36 (containing only the cloning vector) was used, since there was no change in the size of inhibition zones in comparison with negative control reactions. Crude extracts from M. smegmatis SAN19 [which produces APH(3")-Ic] reduced the size of the inhibition zone, indicating that the antimicrobial activity of the STR solution had been reduced by approximately 50%. This confirms that APH(3")-Ic is capable of inactivating STR, providing these strains with an effective STR resistance mechanism. It should be noted that the possibility of acquiring STR resistance through 16S rRNA mutations in M. fortuitum (which has two rRNA operons) would be much less than that in M. tuberculosis (which has only one). Also, APH(3")-Ic could have an additional role in protein phosphorylation or other metabolic processes, as has been suggested for APH enzymes (4, 18).

Distribution of the aph(3")-Ic gene in M. fortuitum and other mycobacteria. The aminoglycoside-2'-N-acetyltransferase gene from M. fortuitum is universally present in this species (3), and most mycobacterial species have similar genes. Using Southern blot hybridization, we detected the aph(3")-Ic gene in 4 out of 22 M. fortuitum clinical isolates from our culture collection. Those isolates containing the aph(3")-Ic gene were resistant to STR, three of them showing STR MICs of 50 µg/ml, which is the highest level of STR resistance we have detected in our collection of M. fortuitum isolates. However, some M. fortuitum isolates lacking this gene were also resistant to STR. We can therefore conclude that APH(3")-Ic contributes to STR resistance, but most likely there could be many other yet unknown mechanisms that also contribute to STR resistance.

Homologues of the aph(3")-Ic gene could not be found in databases of mycobacterial genomes such as M. tuberculosis H37Rv and M. leprae (both at http://genolist.pasteur.fr/) or M. smegmatis (http://www.tigr.org/). This gene therefore seems to be exclusive to some M. fortuitum strains. We can speculate that these strains might have a common M. fortuitum ancestor that acquired the aph(3")-Ic gene from plasmid RSF1010, since under laboratory conditions this plasmid and its derivatives can replicate in mycobacteria (6, 9), perhaps facilitated by the high GC content of RSF1010 (60.9%), which is similar to that of mycobacterial genomes.

Physical location of the M. fortuitum aph(3")-Ic gene. Genes encoding aminoglycoside-modifying enzymes can be located either in plasmids or in the bacterial chromosome. Plasmids are frequently found in M. fortuitum isolates (11), but a chromosomally located aminoglycoside-N-acetyltransferase also has been characterized (3). We assume the aph(3")-Ic gene is located in the chromosome of M. fortuitum for two reasons. First, using PCR, the aph(3")-Ic gene could not be amplified from a 16-kb plasmid identified previously in the M. fortuitum FC1 strain (5). Second, genes flanking it are similar to chromosomal genes from M. tuberculosis, and this region is very similar to a locus in the chromosome of M. smegmatis (Fig. 1).

Nucleotide sequence accession number. The nucleotide sequence determined in this work has been deposited in the GenBank, EMBL, and DDBJ databases under the accession number DQ336355.


   ACKNOWLEDGMENTS
 
This work was supported by CIBER grant CB06/06/0020 from the Spanish Ministerio de Sanidad y Consumo, EU grant QLK2-CT-2000-01761, and Spanish Ministerio de Educación y Ciencia (MEC) grants BIO2002-01297 and BIO 2005-01801. J.A.A. is a recipient of a "Ramon y Cajal" fellowship. S.R.-G. holds a fellowship from MEC (AP2001-1114).

We acknowledge P. Goñi for critical reading of the manuscript.


   FOOTNOTES
 
* Corresponding author. Mailing address: Departamento de Microbiología, Medicina Preventiva y Salud Pública, Facultad de Medicina, Universidad de Zaragoza, C/ Domingo Miral s/n, 50009-Zaragoza, Spain. Phone: 34-976-762420. Fax: 34-976-762604. E-mail: ainsa{at}unizar.es. Back

{triangledown} Published ahead of print on 5 September 2006. Back


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Antimicrobial Agents and Chemotherapy, November 2006, p. 3920-3922, Vol. 50, No. 11
0066-4804/06/$08.00+0     doi:10.1128/AAC.00223-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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