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Antimicrobial Agents and Chemotherapy, February 2006, p. 748-751, Vol. 50, No. 2
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.2.748-751.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Efficacy of Quinolones against Secondary Pneumococcal Pneumonia after Influenza Virus Infection in Mice

Katsuhiko Hayashi, Shin-etsu Kadowaki,* Masaya Takei, and Hideyuki Fukuda

Discovery Research Laboratories, Kyorin Pharmaceutical Co., Ltd., Nogi, Shimotsuga, Tochigi 329-0114, Japan

Received 8 July 2005/ Returned for modification 18 July 2005/ Accepted 13 November 2005


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ABSTRACT
 
We established a mouse model of secondary pneumococcal pneumonia after influenza virus infection and investigated the efficacy of several quinolones against pneumonia in this model. Gatifloxacin exhibited the highest efficacy among the quinolones examined and is probably useful for the treatment of secondary bacterial pneumonia.


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TEXT
 
Influenza virus is a frequent cause of severe acute respiratory infection. According to studies conducted during influenza epidemics, influenza virus infection is one of the causes of excess mortality worldwide (1, 2, 4). The most severe condition associated with influenza virus infection is pneumonia, which is often life threatening (12, 13). Although influenza virus infection by itself can lead to pneumonia, secondary bacterial infection during or shortly after recovery from the influenza virus infection is a much more common cause of pneumonia (4, 7, 15). While anti-influenza virus drugs, such as amantadine, zanamivir, and oseltamivir, have been used for the treatment of influenza virus infection, these agents show no efficacy against secondary bacterial infections. Recently, the respiratory quinolones have been used successfully for the treatment of pneumonia caused by bacteria (3). Gatifloxacin is a respiratory quinolone with broad-spectrum antibacterial activity against pathogens implicated in respiratory infections and is recommended for use in the treatment of community-acquired pneumonia (5). On the other hand, the efficacy of gatifloxacin against secondary bacterial pneumonia has not yet been well studied. In this study, to evaluate the efficacies of quinolones, including gatifloxacin, we established and characterized a mouse model of secondary pneumococcal pneumonia after influenza virus infection.

The secondary pneumococcal pneumonia model was established using 6-week-old female BALB/c mice infected with influenza virus A/PR/8/34 (H1N1) and Streptococcus pneumoniae KY9, both of which exhibit high virulence in mice. The mice were lightly anesthetized by intraperitoneal injection of pentobarbital at 1.25 mg/mouse and inoculated intranasally with 20 PFU of influenza virus. Seven days after the influenza virus inoculation, the mice were anesthetized by intraperitoneal injection of pentobarbital at 0.94 mg/mouse and then intratracheally inoculated with S. pneumoniae (approximately 105 CFU/mouse). All of the mice infected with either influenza virus or S. pneumoniae alone survived for at least 10 days after inoculation. In contrast, all of the mice inoculated with S. pneumoniae 7 days after the influenza virus inoculation died within 4 days of the bacterial inoculation.

The bacterial titers in the lungs of mice infected with 8.2 x 104 CFU/lung of S. pneumoniae alone were reduced to below the limit of detection (<5.0 x 102 CFU/lung) 4 days after the bacterial inoculation. On the other hand, the bacterial titers of mice infected secondarily with S. pneumoniae after the influenza virus inoculation increased to 6.6 x 107 CFU/lung 1 day after the bacterial inoculation and remained at this level.

Figure 1 shows representative histological examples of lung tissue samples from infected mice. The lung injury caused by the secondary bacterial pneumonia (Fig. 1E and F) was significantly more extensive than that caused by influenza virus (Fig. 1A and B) or S. pneumoniae (Fig. 1C and D) infection alone. The secondary bacterial pneumonia was characterized by extensive inflammatory cell infiltration, focal necrosis, and hemorrhage.


Figure 1
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  		FIG. 1. Pathological examination of lung tissue specimens of mice infected with influenza virus alone (A, B), S. pneumoniae alone (C, D), and S. pneumoniae after influenza virus infection (E, F). The organs were stained with hematoxylin-eosin. Magnifications, x40 (A, C, E) and x100 (B, D, F).

These results indicate that the susceptibility of the mice to S. pneumoniae increased markedly by influenza virus infection. Recently, an animal model of secondary bacterial pneumonia after influenza virus infection was established (8, 14). It has been reported that bacterial adhesion to the epithelial cells of the bronchial mucosa is enhanced by influenza virus infection (6, 11) and that virus-mediated alterations in the receptor milieu can create circumstances favorable to pneumococcal infection; for example, viral neuraminidase helps to prime the lungs for pneumococcal infection (9). These findings lend support to our experimental results. In this model, pneumococcal pneumonia was induced in nonimmunocompromised mice, in contrast to the conventional model in which leukopenia is induced in the mice by pretreatment with chemical compounds, such as cyclophosphamide. Therefore, it is suggested that this secondary bacterial pneumonia model may represent human pneumococcal pneumonia better.

In the present work, we evaluated the therapeutic effects of three quinolones—gatifloxacin, levofloxacin, and ciprofloxacin using the secondary infection model of mice infected with the KY9 strain of S. pneumoniae (penicillin-susceptible S. pneumoniae; penicillin G MIC, 0.063 µg/ml). The MICs of gatifloxacin, levofloxacin, and ciprofloxacin against KY9 were 0.25, 1, and 1 µg/ml, respectively. The quinolones were administered orally at a dose of 25, 50, or 100 mg/kg body weight twice daily for 3 days, beginning 18 h after inoculation of S. pneumoniae. The mice were monitored daily for mortality until 10 days after the bacterial inoculation. Administration of gatifloxacin at doses of 25, 50, and 100 mg/kg induced a significant prolongation of survival in a dose-dependent manner (P < 0.01; Fig. 2, left). In addition, the bacterial titers in the lungs of the mice treated with 25, 50, and 100 mg/kg of gatifloxacin also declined to 7.8 x 106, 9.5 x 103, and 9.5 x 102 CFU/lung, respectively, 6 days after the bacterial inoculation. The mice treated with levofloxacin at 100 mg/kg also showed significant prolongation of survival (P < 0.01), although those treated with 25 and 50 mg/kg of levofloxacin died within 7 days of the bacterial inoculation. All of the ciprofloxacin-treated mice died within 4 days of the bacterial inoculation. Similar results were obtained following secondary infection using another strain of S. pneumoniae, MBC188, categorized into penicillin-intermediate S. pneumoniae (penicillin G MIC, 0.5 µg/ml; Fig. 2, right). The MICs of gatifloxacin, levofloxacin, and ciprofloxacin against MBC188 were 0.5, 2, and 2 µg/ml, respectively. Thus, our results revealed that gatifloxacin was more effective than either levofloxacin or ciprofloxacin against infection induced by either strain of S. pneumoniae in our secondary pneumococcal infection model.


Figure 2
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  		FIG. 2. Cumulative survival rates of the infected mice treated with gatifloxacin (A), levofloxacin (B), and ciprofloxacin (C). Mice were infected with influenza virus and then with the KY9 (left) or MBC188 (right) strain of S. pneumoniae. Arrows indicate the times of administration of quinolones. Symbols: •, control; {diamondsuit}, 25 mg/kg; {blacktriangleup}, 50 mg/kg; {blacksquare}, 100 mg/kg. The survival rates of each group of mice were compared with those of the control groups using the log rank test. Values that were significantly different from those of the control group are indicated by asterisks (**, P < 0.01; ***, P < 0.001).

There have been few reports on the efficacies of antibacterial agents in the secondary bacterial pneumonia model (10). In the present work, several of the quinolones examined showed efficacy in vivo in our secondary infection model. Therefore, it appears that this model may be useful for evaluation of the efficacy of antibacterial agents against secondary bacterial pneumonia. Gatifloxacin exhibited the greatest efficacy among the quinolones examined in this model and is, therefore, probably useful for the treatment of pneumonia, including secondary bacterial infection. Treatment with appropriate antibacterial agents is necessary for cure in cases of secondary bacterial pneumonia.


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FOOTNOTES
 
* Corresponding author. Mailing address: Discovery Research Laboratories, Kyorin Pharmaceutical Co., Ltd., 2399-1, Nogi, Shimotsuga, Tochigi 329-0114, Japan. Phone: 81-280-56-2201. Fax: 81-280-57-1293. E-mail: shinetsu.kadowaki{at}mb.kyorin-pharm.co.jp. Back


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Antimicrobial Agents and Chemotherapy, February 2006, p. 748-751, Vol. 50, No. 2
0066-4804/06/$08.00+0     doi:10.1128/AAC.50.2.748-751.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Choi, Y. S., Baumgarth, N. (2008). Dual role for B-1a cells in immunity to influenza virus infection. JEM 205: 3053-3064 [Abstract] [Full Text]  

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