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Previous Article | Next Article 
Antimicrobial Agents and Chemotherapy, December 2007, p. 4518-4520, Vol. 51, No. 12
0066-4804/07/$08.00+0 doi:10.1128/AAC.00808-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Institute of Infectious Diseases and Public Health, Polytechnic University of the Marche Region, A.O. Ospedali Riuniti, Ancona, Italy,1 Department of General Surgery, I.N.R.C.A.-I.R.R.C.S., Polytechnic University of the Marche Region, Ancona, Italy,2 Institute of Maternal and Children's Sciences—Urology, Polytechnic University of the Marche Region, A.O. Ospedali Riuniti, Ancona, Italy,3 Experimental Animal Models for Aging Units, Research Department, I.N.R.C.A.-I.R.R.C.S., Ancona, Italy,4 Tufts University, Cummings School of Veterinary Medicine, Department of Biomedical Sciences, North Grafton, Massachusetts 015365
Received 22 June 2007/ Returned for modification 17 August 2007/ Accepted 6 September 2007
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RNAIII inhibiting peptide (RIP; YSPWTNF-NH2) inhibits staphylococcal biofilm formation and toxin production (5, 13). In the rat model, RIP has been shown to prevent biofilm formation and staphylococcal infections, whether it was applied to antibiotic-sensitive or antibiotic-resistant strains (6, 8). Its mechanism of action is different from that of common antibiotics, since instead of killing the bacteria, it inhibits cell-to-cell communication, leading to the prevention of cell adhesion and virulence in vivo.
Staphylococcus aureus is one of the common colonizers of urinary stents (14, 15), especially in patients with an indwelling urinary catheter or those who are immunocompromised. The aim of the present study was to assess the efficacy of RIP in the prevention of staphylococcal ureteral stent infections.
The S. aureus strain Smith diffuse (SD), a slime-producing strain with exopolysaccharides which are antigenically identical to many clinical S. aureus strains, was tested.
The MIC was determined according to the procedures outlined by the CLSI (9).
Adult female Wistar rats (weight range, 180 to 250 g) (n = 5) were used. The study included a control group (C0) without a bacterial challenge to evaluate the sterility of the surgical procedure; a challenged control group (C1) without antibiotic prophylaxis; and three challenged groups: (i) a group that received 10 mg/kg of body weight teicoplanin intraperitoneally immediately after stent implantation; (ii) a group that received RIP-coated stents, in which 0.2-cm2 sterile stents (BiosoftDuo, Porges-Mentor, France) were incubated in 1 µg/ml RIP (Neosystem) solution for 30 min immediately before implantation; and (iii) a group that received stents coated with intraperitoneal teicoplanin (Aventis) that were incubated with RIP at the above-cited concentration. Experiments were performed in duplicate. The dose of teicoplanin was equivalent to that usually used in the human clinical setting, while the RIP dose was chosen on the basis of previous reports (1, 2). For statistical analysis, the data were pooled and refer to all 10 animals from each pair of groups.
The rats were anesthetized by an intramuscular injection of ketamine and xylazine (30 mg/kg and 8 mg/kg, respectively), the hair was shaved, and the skin was cleansed with a 10% povidone-iodine solution. The bladder was exposed through a suprapubic incision and opened at the dome (7). After cystotomy, stents were inserted into the bladder. Before implantation was performed, some of the ureteral stent segments were impregnated with RIP as described above. The bladder was sutured with 000 surgical silk. After the surgical intervention, a saline solution (1 ml) containing 2 x 107 CFU/ml S. aureus (SD) (4, 17) was inoculated into the bladder, using a tuberculin syringe. Some of the animals received teicoplanin intraperitoneally, immediately after stent implantation. The animals were returned to individual cages and were thoroughly examined daily. Twenty-four hours after stent placement, urine cultures were performed with a transvesicular sample taken by an insulin syringe to verify sterility or infection. Stents were explanted at day 5 following implantation. Enumeration of viable bacteria was performed by culturing serial 10-fold dilutions (0.1 ml) of the bacterial suspension on blood agar plates. All plates were incubated at 37°C for 48 h. The organisms were quantitated by counting the number of CFU per plate (1). The limit of detection was approximately 10 CFU/ml. Culture results were presented as means ± standard deviations, and statistical comparisons between groups were made using analysis of variance with the log-transformed data with the Tukey-Kramer honestly significant difference test. Significance was accepted when the P value was 
0.05.
Toxicity was evaluated on the basis of the presence of any drug-related adverse effects, i.e., signs of local inflammation, weight loss, diarrhea, fever, and behavioral alterations.
In vitro teicoplanin exhibited a MIC of 1.00 µg/ml, while as expected, RIP did not inhibit growth of the staphylococcal strain.
None of the animals included in the uncontaminated control group had microbiological evidence of stent infection. By contrast, all rats included in the challenged but untreated control group demonstrated evidence of infection, with quantitative culture results showing 6.6 x 106 ± 1.9 x 106 CFU/ml. Rats that received intraperitoneal teicoplanin showed bacterial counts of 3.8 x 103 ± 0.8 x 103 CFU/ml. Animals that had RIP-coated stents showed bacterial counts of 6.7 x 104 ± 1.4 x 103 (P < 0.05). Animals that had RIP-coated stents and were treated with teicoplanin had no bacterial counts (P < 0.001), indicating that RIP combined with teicoplanin showed efficacies that were higher than that of each single compound alone. Urine cultures confirmed these microbiological data and were negative both for the uncontaminated group and the combined treatment group. The single-agent-treated groups were positive, with a bacterial count of 104 CFU/ml both for the RIP- and for the teicoplanin-treated group (Table 1). None of the animals included in any group died or had any clinical evidence of drug-related adverse effects.
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RIP has been shown to be effective against every staphylococcal strain tested so far, including methicillin- and glycopeptide-intermediate-resistant S. aureus and Staphylococcus epidermidis (2, 6), suggesting that RIP-impregnated ureteral stents may benefit from lower rates of infections.
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ACKNOWLEDGMENTS |
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This work was supported by grants from the Italian Ministry of Education, from University and Research (PRIN 2005), and from the NIH (grant AI54858).
The study was approved by the animal research ethics committee of the I.N.R.C.A.-I.R.R.C.S.
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FOOTNOTES |
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Published ahead of print on 17 September 2007.
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