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Antimicrobial Agents and Chemotherapy, April 2007, p. 1563-1565, Vol. 51, No. 4
0066-4804/07/$08.00+0     doi:10.1128/AAC.01326-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Drug Therapy of Experimental Tuberculosis (TB): Improved Outcome by Combining SQ109, a New Diamine Antibiotic, with Existing TB Drugs{triangledown}

Boris V. Nikonenko,1,2* Marina Protopopova,1 Rowena Samala,1 Leo Einck,1 and Carol A. Nacy1

Sequella, Inc., Rockville, Maryland 20850,1 Central Research Institute for Tuberculosis, Moscow 107564, Russia2

Received 24 October 2006/ Returned for modification 27 November 2006/ Accepted 13 January 2007


   ABSTRACT
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Substitution of the new diamine antibiotic SQ109 for ethambutol in a mouse model of chronic tuberculosis (TB) improved efficacy of combination drug therapy with first-line TB drugs rifampin and isoniazid, with or without pyrazinamide: at 8 weeks, lung bacteria were 1.5 log10 lower in SQ109-containing regimens.


   TEXT
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New antituberculosis drug regimens are clearly needed to reduce the therapy time interval required for a durable cure and to treat the expanding problem of drug- and multidrug-resistant (MDR) Mycobacterium tuberculosis strains. Several new drug candidates in development show comparable or better performance than currently available antitubercular agents both in vitro and in animal models of tuberculosis (TB) and are effective against MDR mycobacterial strains (1, 5, 11, 16, 17). For the past several years, we have been characterizing the properties of a new diamine drug candidate with antitubercular activity, SQ109 (4, 7, 8, 10, 14). This new drug with a cell wall mode of action has excellent in vitro activity against both drug-sensitive, single-drug-resistant, and MDR M. tuberculosis strains (more than 50, including laboratory strains and clinical isolates), with a MIC range of 0.16 to 0.64 µg/ml against all M. tuberculosis isolates tested. Moreover, SQ109 by itself at 10 mg/kg of body weight was able to reduce the number of lung CFU by over 1.5 to 2 log10 in a chronic mouse model of TB, activity that was similar to monotherapy with ethambutol (EMB) at 100 mg/kg (7, 14).

Recently we reported marked in vitro synergistic activity of SQ109 with isoniazid (INH) and rifampin (RIF) and additive activity with streptomycin (4). In preliminary studies to evaluate any synergistic activity with front-line TB drugs in vivo, we used a screening mouse model that followed weight loss as an indicator of TB severity (2, 12). Similar to in vitro observations of synergy, combination therapy with SQ109-containing regimens was also better at preventing TB-induced weight loss than the standard drug regimens (13).

In this report, we compared the efficacy of TB therapy with three- and four-drug combinations where SQ109 replaced EMB in the more standard chronic mouse model of TB.

Groups of C57BL/6 female mice were inoculated intravenously with M. tuberculosis H37Rv and treated each day for 5 days/week with saline (untreated controls; six mice/time point), individual drugs (six mice/time point), or drug combinations (six mice/time point) beginning 3 weeks after infection and continuing for 4 or 8 weeks. To assess efficacy of treatment, we evaluated the average number of M. tuberculosis CFU in lungs of six mice/treatment each week during the course of therapy.

In the first set of experiments, we evaluated CFU in lungs of mice infected with 5 x 104 CFU M. tuberculosis H37Rv and treated with INH alone or with a combination of INH plus RIF, INH plus RIF plus EMB, or INH plus RIF plus SQ109 over 4 weeks (Table 1). INH was used at 25 mg/kg; RIF was used at 20 mg/kg; EMB was used at its minimal effective dose in mice, 100 mg/kg; SQ109 was used at its minimum effective dose, 10 mg/kg. The combination of INH plus RIF plus SQ109 was better than and statistically different from INH plus RIF plus EMB at weeks 2, 3, and 4. At 4 weeks, the average number of viable M. tuberculosis in lungs of untreated mice was 6.42 log10 CFU; lungs of mice treated with INH contained an average 4.61 log10 CFU, the INH plus RIF group had an average 4.27 log10 CFU, the INH plus RIF plus EMB group had 3.86 log10 CFU, and the INH plus RIF plus SQ109 group had 3.26 log10 CFU, 0.6 log10 lower than the standard drug combination (Table 1). In terms of CFU counts, that half-log decrease in CFU translated into a fourfold decrease in the number of viable bacteria: 7,330 in mice treated with INH plus RIF plus EMB versus 1,830 in mice treated with INH plus RIF plus SQ109. Moreover, the combination of INH plus RIF plus SQ109 resulted in the same CFU level in lungs at week 3 as in the INH plus RIF plus EMB group at week 4, a full week earlier. Two other 4-week experiments with the identical three-drug regimen demonstrated similar results: differences between log10 CFU of mice treated with INH plus RIF plus EMB and INH plus RIF plus SQ109 were 0.44 and 0.50 (experiments 2 and 3, respectively), with the SQ109-containing regimen showing better elimination of bacteria from the lung.


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  		TABLE 1. CFU in lungs of mice treated for 4 weeks with a three-drug regimena

 
In the second series of experiments, pyrazinamide (PZA; 150 mg/kg) was added to INH, RIF, EMB, or SQ109 (concentrations described above) in order to study the complete standard four-drug regimen used in the intensive phase of TB therapy in humans. Efficacy of the EMB-containing combination was compared to efficacy of the SQ109-containing combination. Figure 1 shows the changes in CFU in lungs of control mice and mice over 8 weeks of treatment with INH plus RIF plus EMB plus PZA or INH plus RIF plus SQ109 plus PZA. As above, treatment was initiated 3 weeks following infection of mice with 105 CFU M. tuberculosis H37Rv, a time when the level of CFU in lungs reaches a plateau. Four-week therapy with INH plus RIF plus EMB plus PZA reduced log10 CFU in lungs to 4.11, while INH plus RIF plus SQ109 plus PZA reduced log10 CFU to 3.48, a nearly 0.6 log10 difference. In untreated mice at 4 weeks, the average log10 CFU was 7.00. After 6 weeks of therapy with INH plus RIF plus EMB plus PZA, the average log10 CFU in lungs was 3.42, and in mice treated with INH plus RIF plus SQ109 plus PZA, the average lung CFU count was 1.92 log10 (over a 1.5-log10 difference). Untreated mice at 6 weeks again had an average CFU count in lungs of 7.00 log10. After 8 weeks of therapy, INH plus RIF plus SQ109 plus PZA almost eradicated infection (an average of 18 CFU on plates of undiluted whole lung tissue; bacilli were not detected in undiluted lung homogenate samples of two of the six mice), whereas INH plus RIF plus EMB plus PZA had an average of 580 CFU, a 32-fold higher number of bacteria than the SQ109-containing regimen. The average log10 CFU of untreated mice was 7.08 at week 8 (Fig. 1). These results were reproduced in two other experiments with similar differences between SQ109- and EMB-containing regimens.


Figure 1
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  		FIG. 1. Efficacy of four-drug combination treatment of mice infected with M. tuberculosis H37Rv. Differences between CFU recovered from lungs of mice treated with the EMB-containing regimen and the SQ109-containing regimen were significant (4-week treatment, P = 0.001; 6-week treatment, 1.5 log10 CFU difference, P = 0.022; 8-week treatment, 1.5 log10 CFU difference, P < 0.001). Ó, INH (25 mg/kg) plus RIF (20 mg/kg).

 
SQ109 affects synthesis of M. tuberculosis cell walls (10, 14). Although the precise target(s) for SQ109 activity is not yet known, some of the genes switched on or off in M. tuberculosis by SQ109 are the same as EMB, but some are different (3). The importance of the differences between EMB-regulated and SQ109-regulated genes is underscored by the strong synergy between RIF/INH and SQ109 that is not present in the combination of RIF and EMB, either in vitro or in vivo. Moreover, the MIC range of SQ109 does not change for EMBr strains or MDR strains compared to drug-sensitive strains of M. tuberculosis (3, 14), signifying a second important difference between SQ109 and cell wall-active EMB.

The combination of SQ109 with INH, RIF, and PZA in this study provided a new and very effective anti-TB intensive phase treatment regimen that achieved a better and faster rate of mycobacterial kill than the therapeutic regimen of INH plus RIF plus EMB plus PZA. The long half-life of SQ109 in vivo (26 h in beagle dogs [6]) suggests that replacing RIF with longer-acting rifamycins that can be administered weekly in SQ109 combination therapies might also improve the timing of drug delivery for a TB therapy that includes SQ109 (9, 15). The half-life of SQ109 in humans is not yet known, but ongoing phase 1 safety studies of SQ109 in human volunteers will provide these data in the next several months.


   ACKNOWLEDGMENTS
 
This work was generously supported by the National Institutes of Health, NIAID challenge grant UC1 AI-049514-01 ("Second generation antibiotics from ethambutol"), SBIR phase I grant R43 AI060250-01 ("Development of dipiperidines as a new class of anti-TB drugs"), and corporate matching funds.


   FOOTNOTES
 
* Corresponding author. Mailing address: Sequella, Inc., 9610 Medical Center Drive, Suite 200, Rockville, MD 20850. Phone: (301) 762-7776. Fax: (301) 762-7778. E-mail: borisnikonenko{at}sequella.com Back

{triangledown} Published ahead of print on 22 January 2007. Back


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Antimicrobial Agents and Chemotherapy, April 2007, p. 1563-1565, Vol. 51, No. 4
0066-4804/07/$08.00+0     doi:10.1128/AAC.01326-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.




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