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FIG. 1. Detection of E. canis in canine blood after doxycycline treatment. An E. canis-specific PCR assay was used to test buffy coat samples collected from subclinical experimentally infected dogs through the course of doxycycline therapy as described in the text. For each row, no-template reactions (N) served as contamination controls, template DNA (100 pg) collected from E. canis-infected DH82 cells served as a positive control (+), and the numbers 0 to 28 indicate days after initiation of doxycycline therapy. The molecular size standard (M) is a 100-bp ladder (Invitrogen). Doxycycline treatment was initiated after blood collection on day 0 and continued through day 14, which is indicated by the solid bars. Lanes containing the 200-bp amplicon (arrowhead) were considered PCR positive. Dog AXM served as the specific-pathogen-free control, with the numbers 0 to 42 representing days after attachment of uninfected nymphs that were cohorts of those allowed to acquisition feed on the dogs infected with E. canis.

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