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Antimicrobial Agents and Chemotherapy, February 2008, p. 810, Vol. 52, No. 2
0066-4804/08/$08.00+0     doi:10.1128/AAC.01501-07

AUTHOR'S CORRECTION

First Report of the Emergence of CTX-M-Type Extended-Spectrum β-Lactamases (ESBLs) as the Predominant ESBL Isolated in a U.S. Health Care System

Department of Pharmacy, University Health System, San Antonio, Texas, and Departments of Microbiology, Medicine, and Pathology, University of Texas Health Science Center, San Antonio, Texas 78229

Vol. 51, no. 11, p. 4015-4021, 2007. Subsequent to the publication of our article, it was recognized that some details of our PCR and sequencing procedures were not thoroughly described.

Page 4016, column 1: Lines 17-30 should read as follows. "Amplicons to be sequenced (high-fidelity PCRs) were prepared using Triple Master Taq polymerase (Eppendorf, Westbury, NY) according to the manufacturer's instructions. The high-fidelity PCRs consisted of a final concentration of 2.5 units of Taq polymerase, 5 µl of the template DNA, 200 nM of each primer, 200 µM dNTP, 1x buffer with magnesium, and distilled water to achieve a final volume of 50 µl. Screening PCRs (nonsequenced) were prepared with Taq polymerase from Invitrogen (Carlsbad, CA) using component concentrations recommended by the manufacturer for the basic PCR protocol. Reactions were run in an MJ mini thermocycler (Bio-Rad Laboratories, Hercules, CA), using a basic cycling program of 94°C for 2 min (first cycle only), 94°C for 15 s, the primer-specific annealing temperature (see references from Table 1) for 30 s, 72°C for 30 s (30 cycles), and 72°C for 3 min. The primers are shown in Table 1 and were synthesized at the Advanced Nucleic Acids Core Facility at the University of Texas Health Science Center at San Antonio. The primers for CTX-M group 3 were redesigned (CTX-M8.WSAgroupIII.F and CTX-M8.WSAgroupIII.R; annealing temperature, 65°C) using sequences from GenBank (accession numbers X92506, AF189721, X92507, and AF252622)."

Page 4016, column 2: Lines 4-13 should read as follows. "The predicted PCR product was a 540-bp intragenic fragment of bla_OXA-1. PCRs were performed in a DNA thermal cycler (Eppendorf) and prepared as described above using Triple Master Taq polymerase (Eppendorf). Thirty-five cycles were performed for each reaction using the following temperature profile: 94°C for 1 min, 57°C for 1 min, and 72°C for 1 min. All PCR products from the isolates were sequenced. The sequence of the bla_OXA-30 gene was deposited under Genbank accession number AF255921."

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lewis, J. S. Articles by Jorgensen, J. H. Search for Related Content PubMed Articles by Lewis, J. S., II Articles by Jorgensen, J. H.